{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T03:56:03Z","timestamp":1790913363975,"version":"4.1.0"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2008,4,1]],"date-time":"2008-04-01T00:00:00Z","timestamp":1207008000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2008,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Nocardia<\/jats:italic>\n            isolates that share the property of in vitro amikacin resistance are grouped together by some authors in the\n            <jats:italic>Nocardia transvalensis<\/jats:italic>\n            complex. Our examination of 13 isolates that are amikacin resistant has revealed the existence of three distinct species. Sequence analysis of the 16S rRNA, 65-kDa heat shock protein, and\n            <jats:italic>secA1<\/jats:italic>\n            genes, coupled with DNA-DNA hybridization, indicated that \u201c\n            <jats:italic>N. asteroides<\/jats:italic>\n            drug pattern IV,\u201d \u201c\n            <jats:italic>N. transvalensis<\/jats:italic>\n            new taxon 1,\u201d and\n            <jats:italic>N. transvalensis<\/jats:italic>\n            sensu stricto should each be considered a distinct species. The phenotypic and molecular characteristics of the proposed new species\n            <jats:italic>Nocardia wallacei<\/jats:italic>\n            (\n            <jats:italic>N. asteroides<\/jats:italic>\n            drug pattern IV) and\n            <jats:italic>N. blacklockiae<\/jats:italic>\n            (\n            <jats:italic>N. transvalensis<\/jats:italic>\n            new taxon 1) are presented and compared with those of\n            <jats:italic>N. transvalensis<\/jats:italic>\n            sensu stricto. The relative genetic diversity of isolates best placed with the species\n            <jats:italic>N. blacklockiae<\/jats:italic>\n            is also discussed. Case studies demonstrating the pathogenicity of\n            <jats:italic>N. wallacei<\/jats:italic>\n            and\n            <jats:italic>N. blacklockiae<\/jats:italic>\n            are presented. The type strain of\n            <jats:italic>N. wallacei<\/jats:italic>\n            is ATCC 49873 (DSM 45136), and that of\n            <jats:italic>N. blacklockiae<\/jats:italic>\n            is ATCC 700035 (DSM 45135).\n          <\/jats:p>","DOI":"10.1128\/jcm.02011-07","type":"journal-article","created":{"date-parts":[[2008,2,7]],"date-time":"2008-02-07T02:10:06Z","timestamp":1202350206000},"page":"1178-1184","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":64,"title":["<i>Nocardia wallacei<\/i>\n            sp. nov. and\n            <i>Nocardia blacklockiae<\/i>\n            sp. nov., Human Pathogens and Members of the \u201c\n            <i>Nocardia transvalensis<\/i>\n            Complex\u201d"],"prefix":"10.1128","volume":"46","author":[{"given":"Patricia S.","family":"Conville","sequence":"first","affiliation":[{"name":"Microbiology Service, Department of Laboratory Medicine, Warren G. Magnuson Clinical Center, National Institutes of Health, U.S. Department of Health and Human Services, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"June M.","family":"Brown","sequence":"additional","affiliation":[{"name":"Bacterial Zoonoses Branch, Division of Foodborne, Bacterial and Mycotic Diseases, National Center for Zoonotic, Vector-Borne, and Enteric Diseases, Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arnold G.","family":"Steigerwalt","sequence":"additional","affiliation":[{"name":"Bacterial Zoonoses Branch, Division of Foodborne, Bacterial and Mycotic Diseases, National Center for Zoonotic, Vector-Borne, and Enteric Diseases, Centers for Disease Control and Prevention, U.S. Department of Health and Human Services, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara A.","family":"Brown-Elliott","sequence":"additional","affiliation":[{"name":"University of Texas Health Center, Department of Microbiology, Tyler, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frank G.","family":"Witebsky","sequence":"additional","affiliation":[{"name":"Microbiology Service, Department of Laboratory Medicine, Warren G. Magnuson Clinical Center, National Institutes of Health, U.S. Department of Health and Human Services, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/am.25.4.665-681.1973"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.18.4.816-824.1983"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.15.6.1133-1140.1982"},{"key":"e_1_3_2_5_2","unstructured":"Susceptibility testing of mycobacteria nocardiae and other aerobic actinomycetes; approved standard M24-A. 2003"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.11.5139-5145.2004"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.6.2560-2568.2003"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.1.158-164.2000"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02482-06"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00155-06"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.5694\/j.1326-5377.1992.tb121509.x"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1099\/00221287-109-1-69"},{"key":"e_1_3_2_13_2","unstructured":"Public health mycobacteriology: a guide for the level III laboratory. 1985"},{"key":"e_1_3_2_14_2","first-page":"1458","volume":"2","year":"1986","unstructured":"Lechevalier, H. 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