{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T22:24:23Z","timestamp":1744064663136},"reference-count":54,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2009,5,1]],"date-time":"2009-05-01T00:00:00Z","timestamp":1241136000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The diversity of spirochetes in primary endodontic infections of teeth with chronic apical periodontitis or acute apical abscesses was investigated using 16S rRNA gene clone library analysis. The prevalences of three common cultivable oral\n            <jats:italic>Treponema<\/jats:italic>\n            species were also determined using species-specific nested PCR. All detected spirochetes belonged to the genus\n            <jats:italic>Treponema<\/jats:italic>\n            . Overall, 28 different taxa were identified from the 431 clones sequenced: 9 cultivable and validly named species, 1 cultivable as-yet-uncharacterized strain, and 18 as-yet-uncultivated phylotypes, 17 of which were novel. The large majority of clones (94%) were from cultivable named species. The numbers of\n            <jats:italic>Treponema<\/jats:italic>\n            species\/phylotypes per selected positive sample ranged from 2 to 12. Species-specific nested PCR detected\n            <jats:italic>T. denticola<\/jats:italic>\n            ,\n            <jats:italic>T. socranskii<\/jats:italic>\n            , and\n            <jats:italic>T. maltophilum<\/jats:italic>\n            in 59 (66%), 33 (37%), and 26 (29%) of the 90 cases of primary endodontic infections, respectively. Clone library analysis revealed diverse\n            <jats:italic>Treponema<\/jats:italic>\n            species\/phylotypes as part of the microbiota associated with asymptomatic and symptomatic (abscess) endodontic infections. Although several as-yet-uncultivated\n            <jats:italic>Treponema<\/jats:italic>\n            phylotypes were disclosed, including novel taxa, cultivable named species were more abundant and frequently detected.\n          <\/jats:p>","DOI":"10.1128\/jcm.02016-08","type":"journal-article","created":{"date-parts":[[2009,3,5]],"date-time":"2009-03-05T02:37:02Z","timestamp":1236220622000},"page":"1352-1357","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":31,"title":["Diversity of Spirochetes in Endodontic Infections"],"prefix":"10.1128","volume":"47","author":[{"given":"Mitsuo","family":"Sakamoto","sequence":"first","affiliation":[{"name":"Microbe Division\/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"Jr.","given":"Jose\u0301 F.","family":"Siqueira","sequence":"additional","affiliation":[{"name":"Department of Endodontics and Molecular Microbiology Laboratory, Esta\u0301cio de Sa\u0301 University, Rio de Janeiro, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Isabela N.","family":"Ro\u0302c\u0327as","sequence":"additional","affiliation":[{"name":"Department of Endodontics and Molecular Microbiology Laboratory, Esta\u0301cio de Sa\u0301 University, Rio de Janeiro, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshimi","family":"Benno","sequence":"additional","affiliation":[{"name":"Microbe Division\/Japan Collection of Microorganisms, RIKEN BioResource Center, Wako, Saitama, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/25.17.3389"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.9.3334-3340.2002"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1996.tb00180.x"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/00004770-200312000-00002"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.5.1889-1895.1994"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkg039"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-302x.2000.150308.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2672.2003.01901.x"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/17.19.7843"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0757.2005.00108.x"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.2005.00227.x"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2006.05.003"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.joen.2007.05.017"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.2007.00378.x"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1067\/moe.2001.117263"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.25.14547"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.8.3944-3955.2005"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/17.12.1244"},{"key":"e_1_3_2_20_2","first-page":"379","year":"2004","unstructured":"Maiwald, M. 2004. 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