{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T08:53:04Z","timestamp":1790671984558,"version":"4.1.0"},"reference-count":23,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2010,3,1]],"date-time":"2010-03-01T00:00:00Z","timestamp":1267401600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Definitive identification of\n            <jats:italic>Brucella<\/jats:italic>\n            species remains a challenge due to the high degree of genetic homology shared within the genus. We report the development of a molecular technique which utilizes real-time PCR followed by high-resolution melt (HRM) curve analysis to reliably type members of this genus. Using a panel of seven primer sets, we tested 153\n            <jats:italic>Brucella<\/jats:italic>\n            spp. isolates with &gt;99% accuracy compared to traditional techniques. This assay provides a useful diagnostic tool that can rapidly type\n            <jats:italic>Brucella<\/jats:italic>\n            isolates and has the potential to detect novel species. This approach may also prove helpful for clinical, epidemiological and veterinary investigations.\n          <\/jats:p>","DOI":"10.1128\/jcm.02021-09","type":"journal-article","created":{"date-parts":[[2010,1,7]],"date-time":"2010-01-07T03:04:38Z","timestamp":1262833478000},"page":"697-702","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":51,"title":["Rapid Identification and Discrimination of\n            <i>Brucella<\/i>\n            Isolates by Use of Real-Time PCR and High-Resolution Melt Analysis"],"prefix":"10.1128","volume":"48","author":[{"given":"Jonas M.","family":"Winchell","sequence":"first","affiliation":[{"name":"National Center for Immunization and Respiratory Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard J.","family":"Wolff","sequence":"additional","affiliation":[{"name":"National Center for Immunization and Respiratory Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rebekah","family":"Tiller","sequence":"additional","affiliation":[{"name":"National Center for Zoonotic, Vector-Borne, and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael D.","family":"Bowen","sequence":"additional","affiliation":[{"name":"National Center for Immunization and Respiratory Diseases"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alex R.","family":"Hoffmaster","sequence":"additional","affiliation":[{"name":"National Center for Zoonotic, Vector-Borne, and Enteric Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-3-15"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.32.11.2660-2666.1994"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.12.8353-8361.2005"},{"key":"e_1_3_1_5_2","unstructured":"Code of Federal Regulation: 7 CFR Part 331 9 CFR Part 121 and 42 CFR Part 73. 2005"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2672.2005.02622.x"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1099\/ijs.0.65269-0"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01496-07"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.8.3649-3654.2004"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-8-86"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2008.07.002"},{"key":"e_1_3_1_12_2","first-page":"47","year":"2008","unstructured":"Huynh, L. 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