{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T16:44:10Z","timestamp":1781282650917,"version":"3.54.1"},"reference-count":21,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2011,2,1]],"date-time":"2011-02-01T00:00:00Z","timestamp":1296518400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            With increasing concerns regarding diminishing treatment options for gonorrhea, maintaining the efficacy of currently used treatments and ensuring optimal\n            <jats:named-content content-type=\"genus-species\">Neisseria gonorrhoeae<\/jats:named-content>\n            antimicrobial resistance surveillance are of the utmost importance. Penicillin is still used to treat gonorrhea in some parts of the world. In this study, we developed and validated a real-time PCR assay for the detection of penicillinase-producing\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            (PPNG) in noncultured clinical samples with the aim of enhancing penicillin resistance surveillance. The assay (PPNG-PCR2) was designed to be an indirect marker of penicillinase activity, by targeting a region of sequence predicted to be conserved across all\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            plasmid types harboring the beta-lactamase gene while not specifically targeting the actual beta-lactamase-encoding sequence. The assay was evaluated by using a total of 118\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            clinical isolates and 1,194 clinical specimens, including 239\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            -positive clinical samples from which\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            cells were isolated and for which phenotypic penicillinase results are available. Overall, the PPNG-PCR2 assay provided 100% sensitivity and 98.7% specificity compared to bacterial culture results for the detection of PPNG in clinical specimens. PPNG-PCR2 false-positive results, presumably due to cross-reactions with unrelated bacterial species, were observed for up to 1.3% of clinical samples but could be distinguished on the basis of high cycle threshold values. In tandem with phenotypic surveillance, the PPNG-PCR2 assay has the potential to provide enhanced epidemiological surveillance of\n            <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n            penicillin resistance and is of particular relevance to regions where penicillin is still used to treat gonorrhea.\n          <\/jats:p>","DOI":"10.1128\/jcm.02024-10","type":"journal-article","created":{"date-parts":[[2010,12,16]],"date-time":"2010-12-16T03:51:36Z","timestamp":1292471496000},"page":"513-518","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":34,"title":["Enhancing Gonococcal Antimicrobial Resistance Surveillance: a Real-Time PCR Assay for Detection of Penicillinase-Producing Neisseria gonorrhoeae by Use of Noncultured Clinical Samples"],"prefix":"10.1128","volume":"49","author":[{"given":"Namraj","family":"Goire","sequence":"first","affiliation":[{"name":"Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Children's Health Service District, Queensland, Australia"},{"name":"Clinical Medical Virology Centre, the University of Queensland, Queensland, Australia"},{"name":"Queensland Children's Medical Research Institute, the University of Queensland, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Freeman","sequence":"additional","affiliation":[{"name":"Microbiology Laboratory, Pathology Department, Royal Darwin Hospital, Darwin, Northern Territory, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John W.","family":"Tapsall","sequence":"additional","affiliation":[{"name":"WHO Collaborating Centre for STD and HIV, Microbiology Department, South Eastern Area Laboratory Services, Prince of Wales Hospital, Sydney, New South Wales, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen B.","family":"Lambert","sequence":"additional","affiliation":[{"name":"Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Children's Health Service District, Queensland, Australia"},{"name":"Clinical Medical Virology Centre, the University of Queensland, Queensland, Australia"},{"name":"Queensland Children's Medical Research Institute, the University of Queensland, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael D.","family":"Nissen","sequence":"additional","affiliation":[{"name":"Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Children's Health Service District, Queensland, Australia"},{"name":"Clinical Medical Virology Centre, the University of Queensland, Queensland, Australia"},{"name":"Queensland Children's Medical Research Institute, the University of Queensland, Queensland, Australia"},{"name":"Microbiology Division, Pathology Queensland Central, Royal Brisbane and Women's Hospital Campus, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theo P.","family":"Sloots","sequence":"additional","affiliation":[{"name":"Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Children's Health Service District, Queensland, Australia"},{"name":"Clinical Medical Virology Centre, the University of Queensland, Queensland, Australia"},{"name":"Queensland Children's Medical Research Institute, the University of Queensland, Queensland, Australia"},{"name":"Microbiology Division, Pathology Queensland Central, Royal Brisbane and Women's Hospital Campus, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Whiley","sequence":"additional","affiliation":[{"name":"Queensland Paediatric Infectious Diseases Laboratory, Sir Albert Sakzewski Virus Research Centre, Children's Health Service District, Queensland, Australia"},{"name":"Clinical Medical Virology Centre, the University of Queensland, Queensland, Australia"},{"name":"Queensland Children's Medical Research Institute, the University of Queensland, Queensland, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.44.1.210-212.2000"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1517\/14656560902731993"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1006\/mcpr.1998.0216"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2007.12.007"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00385-09"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/383047"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.183.19.5472-5481.2001"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/45.6.777"},{"key":"e_1_3_2_10_2","first-page":"268","article-title":"Annual report of Australian Gonococcal Surveillance Program. 2008","volume":"33","author":"Tapsall J.","year":"2009","unstructured":"TapsallJ. 2009. Annual report of Australian Gonococcal Surveillance Program. 2008. Commun. Dis. Intell. 33:268\u2013274.","journal-title":"Commun. Dis. Intell"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2008.035337"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1586\/14787210.4.4.619"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1086\/430787"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2005.00053.x"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00020-08"},{"key":"e_1_3_2_16_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-004-1170-0"},{"key":"e_1_3_2_18_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2009.037689"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00306-07"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.2353\/jmoldx.2006.050045"},{"key":"e_1_3_2_22_2","volume-title":"Technical document D007-0408-11","author":"World Health Organization Gonococcal Antimicrobial Surveillance Programme","year":"2008","unstructured":"World Health Organization Gonococcal Antimicrobial Surveillance Programme. 2008. Rationale and applications for the current (2008) WHO panel of Neisseria gonorrhoeae for antimicrobial resistance surveillance for public health purposes, and instructions for their use. Technical document D007-0408-11. WHO Collaborating Centre for STD, Sydney, Australia."}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02024-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02024-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:38:34Z","timestamp":1646483914000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02024-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,2]]},"references-count":21,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2011,2]]}},"alternative-id":["10.1128\/JCM.02024-10"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02024-10","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,2]]},"assertion":[{"value":"2010-09-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-12-08","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-02-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}