{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T03:07:50Z","timestamp":1778036870490,"version":"3.51.4"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2012,12,1]],"date-time":"2012-12-01T00:00:00Z","timestamp":1354320000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2012,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Leishmania<\/jats:named-content>\n            (\n            <jats:named-content content-type=\"genus-species\">Viannia<\/jats:named-content>\n            )\n            <jats:named-content content-type=\"genus-species\">braziliensis<\/jats:named-content>\n            causes three main types of American tegumentary leishmaniasis (ATL), localized cutaneous leishmaniasis (CL), mucosal leishmaniasis (ML), and disseminated leishmaniasis (DL). All forms are observed among individuals of Corte de Pedra, Brazil. We previously used random amplified markers to identify a multiclonal population among\n            <jats:italic>L<\/jats:italic>\n            . (\n            <jats:italic>V<\/jats:italic>\n            .)\n            <jats:named-content content-type=\"genus-species\">braziliensis<\/jats:named-content>\n            isolates from ATL patients, defining parasite clades associated with different clinical syndromes. Herein we compared sequences of random amplified markers to identify genotypes of\n            <jats:italic>L<\/jats:italic>\n            . (\n            <jats:italic>V<\/jats:italic>\n            .)\n            <jats:named-content content-type=\"genus-species\">braziliensis<\/jats:named-content>\n            recovered from lesions of CL, ML, and DL patients. Six polymorphic genomic loci were sequenced from 35 parasite isolates. Single-nucleotide polymorphisms (SNPs) and insertions-deletions (indels) at each locus allowed us to segregate the\n            <jats:italic>L<\/jats:italic>\n            . (\n            <jats:italic>V<\/jats:italic>\n            .)\n            <jats:named-content content-type=\"genus-species\">braziliensis<\/jats:named-content>\n            population according to haplotypes. Several SNPs, indels, and haplotypes were significantly associated with an increased risk of DL. Molecular genotyping may provide markers to identify\n            <jats:italic>L<\/jats:italic>\n            . (\n            <jats:italic>V<\/jats:italic>\n            .)\n            <jats:named-content content-type=\"genus-species\">braziliensis<\/jats:named-content>\n            strains likely to cause this emerging, hard-to-treat form of ATL.\n          <\/jats:p>","DOI":"10.1128\/jcm.02064-12","type":"journal-article","created":{"date-parts":[[2012,10,4]],"date-time":"2012-10-04T05:36:11Z","timestamp":1349328971000},"page":"4028-4034","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":72,"title":["Association between an Emerging Disseminated form of Leishmaniasis and Leishmania (Viannia) braziliensis Strain Polymorphisms"],"prefix":"10.1128","volume":"50","author":[{"given":"Adriano","family":"Queiroz","sequence":"first","affiliation":[{"name":"Servi\u00e7o de Imunologia, Hospital Universit\u00e1rio Professor Edgard Santos, Universidade Federal da Bahia, Salvador, Bahia, 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de Imunologia, Hospital Universit\u00e1rio Professor Edgard Santos, Universidade Federal da Bahia, Salvador, Bahia, Brazil"},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia\u2014Doen\u00e7as Tropicais (INCT-DT), Salvador, Bahia, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paulo Roberto Lima","family":"Machado","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Imunologia, Hospital Universit\u00e1rio Professor Edgard Santos, Universidade Federal da Bahia, Salvador, Bahia, Brazil"},{"name":"Instituto Nacional de Ci\u00eancia e Tecnologia\u2014Doen\u00e7as Tropicais (INCT-DT), Salvador, Bahia, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edgar M.","family":"Carvalho","sequence":"additional","affiliation":[{"name":"Servi\u00e7o de Imunologia, Hospital Universit\u00e1rio Professor Edgard Santos, Universidade Federal da Bahia, Salvador, Bahia, Brazil"},{"name":"Instituto Nacional de Ci\u00eancia e 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