{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T01:17:44Z","timestamp":1790903864332,"version":"4.1.0"},"reference-count":30,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2007,4,1]],"date-time":"2007-04-01T00:00:00Z","timestamp":1175385600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    A rapid two-step DNA extraction method and a multiplex PCR for the detection of dermatophytes in general and\n                    <jats:italic>Trichophyton rubrum<\/jats:italic>\n                    specifically were developed and evaluated with DNA extracted from pure cultures and from clinically diseased nails. DNA from the following dermatophytes was used:\n                    <jats:italic>Epidermophyton floccosum<\/jats:italic>\n                    ,\n                    <jats:italic>Microsporum audouinii<\/jats:italic>\n                    ,\n                    <jats:italic>Microsporum canis<\/jats:italic>\n                    ,\n                    <jats:italic>Microsporum gypseum<\/jats:italic>\n                    ,\n                    <jats:italic>Microsporum nanum<\/jats:italic>\n                    ,\n                    <jats:italic>Trichophyton mentagrophytes<\/jats:italic>\n                    ,\n                    <jats:italic>Trichophyton rubrum<\/jats:italic>\n                    ,\n                    <jats:italic>Trichophyton schoenleinii<\/jats:italic>\n                    ,\n                    <jats:italic>Trichophyton soudanense<\/jats:italic>\n                    ,\n                    <jats:italic>Trichophyton terrestre<\/jats:italic>\n                    ,\n                    <jats:italic>Trichophyton tonsurans<\/jats:italic>\n                    ,\n                    <jats:italic>Trichophyton verrucosum<\/jats:italic>\n                    , and\n                    <jats:italic>Trichophyton violaceum<\/jats:italic>\n                    . Human DNA and DNA from the following nondermatophyte fungi were included as controls:\n                    <jats:italic>Alternaria<\/jats:italic>\n                    ,\n                    <jats:italic>Aspergillus niger<\/jats:italic>\n                    ,\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    ,\n                    <jats:italic>Candida glabrata<\/jats:italic>\n                    ,\n                    <jats:italic>Candida krusei<\/jats:italic>\n                    ,\n                    <jats:italic>Malassezia furfur<\/jats:italic>\n                    ,\n                    <jats:italic>Saccharomyces cerevisiae<\/jats:italic>\n                    , and\n                    <jats:italic>Scopulariopsis brevicaulis.<\/jats:italic>\n                    A total of 118 nail samples received for routine microscopy and culture for dermatophytes were subsequently tested by the two PCRs separately and in a multiplex format. Using DNA extracted from pure cultures and the pan-dermatophyte PCR, the\n                    <jats:italic>T. rubrum<\/jats:italic>\n                    -specific PCR sequentially and in a multiplex format correctly detected all dermatophytes and additionally correctly identified\n                    <jats:italic>T. rubrum.<\/jats:italic>\n                    Comparison of the traditional diagnostic evaluation (microscopy and culture) of nail samples with PCR on DNA directly extracted from the nails showed excellent agreement between PCR and microscopy, but the number of samples with dermatophyte species identification was increased considerably from 22.9% to 41.5%, mainly due to the identification of\n                    <jats:italic>T. rubrum<\/jats:italic>\n                    by PCR in microscopy-positive but culture-negative samples. In conclusion, this 5-hour diagnostic test was shown to increase not only the speed but also the sensitivity of investigation for nail dermatophytosis.\n                  <\/jats:p>","DOI":"10.1128\/jcm.02072-06","type":"journal-article","created":{"date-parts":[[2007,2,2]],"date-time":"2007-02-02T21:42:31Z","timestamp":1170452551000},"page":"1200-1204","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":127,"title":["Five-Hour Diagnosis of Dermatophyte Nail Infections with Specific Detection of\n                    <i>Trichophyton rubrum<\/i>"],"prefix":"10.1128","volume":"45","author":[{"given":"Anna","family":"Brillowska-Da\u0328browska","sequence":"first","affiliation":[{"name":"Unit of Mycology and Parasitology, Statens Serum Institute, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ditte Marie","family":"Saunte","sequence":"additional","affiliation":[{"name":"Unit of Mycology and Parasitology, Statens Serum Institute, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maiken Cavling","family":"Arendrup","sequence":"additional","affiliation":[{"name":"Unit of Mycology and Parasitology, Statens Serum Institute, Copenhagen, Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","unstructured":"Brillowska-D\u0105browska A. December 2006. DNA preparation from nail samples. Denmark patent WO2006133701."},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2005.01122.x"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.11.3.415"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.1997.tb02128.x"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.12.4804-4805.2002"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1080\/00362177185190331"},{"key":"e_1_3_1_8_2","first-page":"101","volume":"67","year":"2003","unstructured":"Hainer, B. L. 2003. Dermatophyte infections. Am. Fam. Physician67:101-108.","journal-title":"Am. Fam. Physician"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1439-0507.1999.00261.x"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2133.2001.00043.x"},{"key":"e_1_3_1_11_2","first-page":"699","volume":"133","year":"1995","unstructured":"Heikkila, H., and S. Stubb. 1995. The prevalence of onychomycosis in Finland. Br. J. Dermatol.133:699-703.","journal-title":"Br. J. Dermatol."},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-4632.2005.02265.x"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-1811(03)00048-3"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-1811(03)00150-6"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1292\/jvms.65.267"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.1998.tb00329.x"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.4.1419-1427.2006"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-49-6-493"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-51-2-117"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0733-8635(03)00035-4"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1159\/000063913"},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1610-0387.2006.05877.x"},{"key":"e_1_3_1_23_2","first-page":"4084","volume":"99","year":"2002","unstructured":"Petrini, B., and M. L. von Rosen. 2002. Optimal dermatophyte diagnosis requires both microscopy and culture. Lakartidningen99:4084.","journal-title":"Lakartidningen"},{"key":"e_1_3_1_24_2","unstructured":"Rippon J. W. 1988. Medical mycology: the pathogenic fungi and the pathogenic actinomycetes. W. B. Saunders Co. Philadelphia PA."},{"key":"e_1_3_1_25_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2004.01066.x"},{"key":"e_1_3_1_26_2","doi-asserted-by":"publisher","DOI":"10.1067\/mjd.2003.491"},{"key":"e_1_3_1_27_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1440-0960.2003.00005.x"},{"key":"e_1_3_1_28_2","doi-asserted-by":"crossref","first-page":"609","DOI":"10.1111\/j.1439-0507.1989.tb02192.x","volume":"32","year":"1989","unstructured":"Summerbell, R. C., J. Kane, and S. Krajden. 1989. Onychomycosis, tinea pedis and tinea manuum caused by non-dermatophytic filamentous fungi. Mycoses32:609-619.","journal-title":"Mycoses"},{"key":"e_1_3_1_29_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1439-0507.2004.00968.x"},{"key":"e_1_3_1_30_2","first-page":"193","volume":"49","year":"2003","unstructured":"Weinberg, J. M., E. K. Koestenblatt, W. D. Tutrone, H. R. Tishler, and L. Najarian. 2003. Comparison of diagnostic methods in the evaluation of onychomycosis. J. Am. Acad. Dermatol.49:193-197.","journal-title":"J. Am. Acad. Dermatol."},{"key":"e_1_3_1_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.8.2.240"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02072-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02072-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,2,10]],"date-time":"2024-02-10T10:45:45Z","timestamp":1707561945000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02072-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,4]]},"references-count":30,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2007,4]]}},"alternative-id":["10.1128\/JCM.02072-06"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02072-06","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,4]]},"assertion":[{"value":"2006-10-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-01-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}