{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T06:04:46Z","timestamp":1791180286138,"version":"4.1.0"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2007,4,1]],"date-time":"2007-04-01T00:00:00Z","timestamp":1175385600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The utility of a genotypic typing assay for\n                    <jats:italic>Legionella pneumophila<\/jats:italic>\n                    was investigated. A multiple-locus variable number of tandem repeats (VNTR) analysis (MLVA) scheme using PCR and agarose gel electrophoresis is proposed based on eight minisatellite markers. Panels of well-characterized strains were examined in a multicenter analysis to validate the assay and to compare its performance to that of other genotyping assays. Excellent typeability, reproducibility, stability, and epidemiological concordance were observed. The MLVA type or profile is composed of a string of allele numbers, corresponding to the number of repeats at each VNTR locus, separated by commas, in a predetermined order. A database containing information from 99\n                    <jats:italic>L. pneumophila<\/jats:italic>\n                    serogroup 1 strains and four strains of other serogroups and their MLVA profiles, which can be queried online, is available from\n                    <jats:ext-link xmlns:xlink=\"http:\/\/www.w3.org\/1999\/xlink\" ext-link-type=\"url\" xlink:href=\"http:\/\/bacterial-genotyping.igmors.u-psud.fr\/\">http:\/\/bacterial-genotyping.igmors.u-psud.fr\/<\/jats:ext-link>\n                    .\n                  <\/jats:p>","DOI":"10.1128\/jcm.02078-06","type":"journal-article","created":{"date-parts":[[2007,1,25]],"date-time":"2007-01-25T00:51:58Z","timestamp":1169686318000},"page":"1190-1199","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":47,"title":["Identification of Variable-Number Tandem-Repeat (VNTR) Sequences in\n                    <i>Legionella pneumophila<\/i>\n                    and Development of an Optimized Multiple-Locus VNTR Analysis Typing Scheme"],"prefix":"10.1128","volume":"45","author":[{"given":"Christine","family":"Pourcel","sequence":"first","affiliation":[{"name":"Univ Paris-Sud, Institut de Ge\u0301ne\u0301tique et Microbiologie, CNRS, Orsay F-91405, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Visca","sequence":"additional","affiliation":[{"name":"Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani I.R.C.C.S"},{"name":"Dipartimento di Biologia, Universita\u0300 Roma Tre, Rome, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baharak","family":"Afshar","sequence":"additional","affiliation":[{"name":"Health Protection Agency, Respiratory and Systemic Infection Laboratory, Centre for Infections, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silvia","family":"D'Arezzo","sequence":"additional","affiliation":[{"name":"Istituto Nazionale per le Malattie Infettive Lazzaro Spallanzani I.R.C.C.S"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gilles","family":"Vergnaud","sequence":"additional","affiliation":[{"name":"Univ Paris-Sud, Institut de Ge\u0301ne\u0301tique et Microbiologie, CNRS, Orsay F-91405, France"},{"name":"Division de Microbiologie Analytique, Centre d'Etudes du Bouchet, Vert le Petit, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Norman K.","family":"Fry","sequence":"additional","affiliation":[{"name":"Health Protection Agency, Respiratory and Systemic Infection Laboratory, Centre for Infections, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.26.9.1695-1703.1988"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1447"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1099776"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-5-4"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.3.506-526.2002"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2005.01224.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.1999.tb00176.x"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-002-0790-5"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s100960000359"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.5.2047-2052.2005"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.7.2932-2939.2003"},{"key":"e_1_3_2_13_2","first-page":"1761","year":"2005","unstructured":"Harrison, T. G. 2005. Legionella, p. 1761-1785. In S. P. Borriello, P. R. Murray, and G. Funke (ed.), Topley & Wilson's Microbiology & Microbial Infections. 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Pourcel. 2006. Multiple locus VNTR (variable number of tandem repeat) analysis (MLVA), p. 83-104. In E. Stackebrandt (ed.), Molecular identification, systematics and population structure of prokaryotes. Springer-Verlag, Berlin, Germany.","journal-title":"Molecular identification, systematics and population structure of prokaryotes"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02078-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02078-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T00:51:52Z","timestamp":1646441512000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02078-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,4]]},"references-count":26,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2007,4]]}},"alternative-id":["10.1128\/JCM.02078-06"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02078-06","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,4]]},"assertion":[{"value":"2006-10-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-01-17","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}