{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T02:16:47Z","timestamp":1791166607123,"version":"4.1.0"},"reference-count":29,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2009,12,1]],"date-time":"2009-12-01T00:00:00Z","timestamp":1259625600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Thirty-eight isolates (including 28 isolates from patients) morphologically identified as\n            <jats:italic>Lichtheimia corymbifera<\/jats:italic>\n            (formerly\n            <jats:italic>Absidia corymbifera<\/jats:italic>\n            ) were studied by sequence analysis (analysis of the internal transcribed spacer [ITS] region of the ribosomal DNA, the D1-D2 region of 28S, and a portion of the elongation factor 1\u03b1 [EF-1\u03b1] gene). Phenotypic characteristics, including morphology, antifungal susceptibility, and carbohydrate assimilation, were also determined. Analysis of the three loci uncovered two well-delimited clades. The maximum sequence similarity values between isolates from both clades were 66, 95, and 93% for the ITS, 28S, and EF-1\u03b1 loci, respectively, with differences in the lengths of the ITS sequences being detected (763 to 770 bp for isolates of clade 1 versus 841 to 865 bp for isolates of clade 2). Morphologically, the shapes and the sizes of the sporangiospores were significantly different among the isolates from both clades. On the basis of the molecular and morphological data, we considered isolates of clade 2 to belong to a different species named\n            <jats:italic>Lichtheimia ramosa<\/jats:italic>\n            because reference strains CBS 269.65 and CBS 270.65 (which initially belonged to\n            <jats:italic>Absidia ramosa<\/jats:italic>\n            ) clustered within this clade. As neotype\n            <jats:italic>A. corymbifera<\/jats:italic>\n            strain CBS 429.75 belongs to clade 1, the name\n            <jats:italic>L. corymbifera<\/jats:italic>\n            was conserved for clade 1 isolates. Of note, the amphotericin B MICs were significantly lower for\n            <jats:italic>L. ramosa<\/jats:italic>\n            than for\n            <jats:italic>L. corymbifera<\/jats:italic>\n            (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.005) but were always \u22640.5 \u03bcg\/ml for both species. Among the isolates tested, the assimilation of melezitose was positive for 67% of the\n            <jats:italic>L. ramosa<\/jats:italic>\n            isolates and negative for all\n            <jats:italic>L. corymbifera<\/jats:italic>\n            isolates. In conclusion, this study reveals that two\n            <jats:italic>Lichtheimia<\/jats:italic>\n            species are commonly associated with mucormycosis in humans.\n          <\/jats:p>","DOI":"10.1128\/jcm.02094-08","type":"journal-article","created":{"date-parts":[[2009,9,17]],"date-time":"2009-09-17T01:46:38Z","timestamp":1253151998000},"page":"3862-3870","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":96,"title":["Molecular and Phenotypic Evaluation of\n            <i>Lichtheimia corymbifera<\/i>\n            (Formerly\n            <i>Absidia corymbifera<\/i>\n            ) Complex Isolates Associated with Human Mucormycosis: Rehabilitation of\n            <i>L. ramosa<\/i>"],"prefix":"10.1128","volume":"47","author":[{"given":"Dea","family":"Garcia-Hermoso","sequence":"first","affiliation":[{"name":"Institut Pasteur, Unite\u0301 de Mycologie Mole\u0301culaire, Centre National de Re\u0301fe\u0301rence Mycologie et Antifongiques, and CNRS URA3012, Paris 75724 Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damien","family":"Hoinard","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unite\u0301 de Mycologie Mole\u0301culaire, Centre National de Re\u0301fe\u0301rence Mycologie et Antifongiques, and CNRS URA3012, Paris 75724 Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Charles","family":"Gantier","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unite\u0301 de Mycologie Mole\u0301culaire, Centre National de Re\u0301fe\u0301rence Mycologie et Antifongiques, and CNRS URA3012, Paris 75724 Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fre\u0301de\u0301ric","family":"Grenouillet","sequence":"additional","affiliation":[{"name":"De\u0301partement de Mycologie-Parasitologie, CHU Jean Minjoz, Besanc\u0327on 25030, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franc\u0327oise","family":"Dromer","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unite\u0301 de Mycologie Mole\u0301culaire, Centre National de Re\u0301fe\u0301rence Mycologie et Antifongiques, and CNRS URA3012, Paris 75724 Cedex 15, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Dannaoui","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unite\u0301 de Mycologie Mole\u0301culaire, Centre National de Re\u0301fe\u0301rence Mycologie et Antifongiques, and CNRS URA3012, Paris 75724 Cedex 15, France"},{"name":"Universite\u0301 Paris Descartes, Faculte\u0301 de Me\u0301decine, AP-HP, Ho\u0302pital Europe\u0301en Georges Pompidou, Unite\u0301 de Parasitologie-Mycologie, Paris 75015, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01685-08"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780701449425"},{"key":"e_1_3_2_4_2","unstructured":"Interpretive criteria for microorganism identification of bacteria and fungi by DNA target sequencing; approved guideline. 2008"},{"key":"e_1_3_2_5_2","first-page":"159","year":"2007","unstructured":"Dannaoui, E., and D. 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