{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T04:18:32Z","timestamp":1790309912125,"version":"4.1.0"},"reference-count":61,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2010,5,1]],"date-time":"2010-05-01T00:00:00Z","timestamp":1272672000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            is a major cause of skin and soft tissue infections, such as furuncles, carbuncles, and abscesses, but it also frequently colonizes the human skin and mucosa without causing clinical symptoms. Panton-Valentine leukocidin (PVL) is a pore-forming toxin that has been associated with soft tissue infections and necrotizing pneumonia. We have compared the genotypes, virulence gene repertoires, and phage patterns of 74 furunculosis isolates with those of 108 control strains from healthy nasal carriers. The large majority of furunculosis strains were methicillin sensitive. Clonal cluster (CC) 121 (CC121) and CC22 accounted for 70% of the furunculosis strains but for only 8% of the nasal isolates. The PVL-encoding genes\n            <jats:italic>luk-PV<\/jats:italic>\n            were detected in 85% of furunculosis strains, while their prevalence among colonizing\n            <jats:italic>S. aureus<\/jats:italic>\n            strains was below 1%.\n            <jats:italic>luk-PV<\/jats:italic>\n            genes were distributed over several lineages (CCs 5, 8, 22, 30, and 121 and sequence type 59). Even within the same lineages,\n            <jats:italic>luk-PV<\/jats:italic>\n            -positive phages characterized furunculosis strains, while their\n            <jats:italic>luk-PV<\/jats:italic>\n            -negative variants were frequent among nasal strains. The very tight epidemiological linkage between\n            <jats:italic>luk-PV<\/jats:italic>\n            and furunculosis, which could be separated from the genetic background of the\n            <jats:italic>S. aureus<\/jats:italic>\n            strain as well as from the gene makeup of the\n            <jats:italic>luk-PV<\/jats:italic>\n            -transducing phage, lends support to the notion of an important role for PVL in human furunculosis. These results make a case for the determination of\n            <jats:italic>luk-PV<\/jats:italic>\n            in recurrent soft tissue infections with methicillin-sensitive as well as methicillin-resistant\n            <jats:italic>S. aureus<\/jats:italic>\n            .\n          <\/jats:p>","DOI":"10.1128\/jcm.02094-09","type":"journal-article","created":{"date-parts":[[2010,3,4]],"date-time":"2010-03-04T02:54:02Z","timestamp":1267671242000},"page":"1527-1535","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":49,"title":["Association of Recurrent Furunculosis with Panton-Valentine Leukocidin and the Genetic Background of\n            <i>Staphylococcus aureus<\/i>"],"prefix":"10.1128","volume":"48","author":[{"given":"Helena","family":"Masiuk","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Pomeranian Medical University, Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katarzyna","family":"Kopron","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Pomeranian Medical University, Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorothee","family":"Grumann","sequence":"additional","affiliation":[{"name":"Institute for Immunology and Transfusion Medicine, University of Greifswald, Greifswald, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christiane","family":"Goerke","sequence":"additional","affiliation":[{"name":"Institute for Medical Microbiology and Hygiene, Universita\u0308tsklinikum Tu\u0308bingen, Tu\u0308bingen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Kolata","sequence":"additional","affiliation":[{"name":"Institute for Immunology and Transfusion Medicine, University of Greifswald, Greifswald, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"Jursa-Kulesza","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Pomeranian Medical University, Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefania","family":"Giedrys-Kalemba","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Pomeranian Medical University, Szczecin, Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barbara M.","family":"Bro\u0308ker","sequence":"additional","affiliation":[{"name":"Institute for Immunology and Transfusion Medicine, University of Greifswald, Greifswald, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silva","family":"Holtfreter","sequence":"additional","affiliation":[{"name":"Institute for Immunology and Transfusion Medicine, University of Greifswald, Greifswald, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-008-0602-7"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.44.2.619-621.2006"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/81385"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.02891.x"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2008.02648.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/592053"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01822-07"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003198"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1086\/342576"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2007.08197.x"},{"key":"e_1_3_2_12_2","first-page":"16","volume":"15","year":"2009","unstructured":"El-Gilany, A. 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