{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,28]],"date-time":"2025-09-28T11:09:48Z","timestamp":1759057788287},"reference-count":17,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2014,10,1]],"date-time":"2014-10-01T00:00:00Z","timestamp":1412121600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>We evaluated the Lyra Direct HSV 1+2\/VZV multiplex real-time PCR assay for the detection and differentiation of herpes simplex virus 1 (HSV-1), HSV-2, and varicella-zoster virus (VZV) on 695 consecutive cutaneous and mucocutaneous lesion specimens. The intra-assay and interassay coefficient of variation values for the Lyra assay were 0.29 to 1.30% and 2.33 to 2.61%, respectively. The sensitivities, specificities, and positive and negative predictive values were 93.4 to 95.0%, 96.1 to 96.8%, 78.0 to 80.3%, and 99.0 to 99.1%, respectively, in comparison to those of viral culture. The values were further improved when a resolution analysis was performed with a laboratory-developed PCR assay.<\/jats:p>","DOI":"10.1128\/jcm.02098-14","type":"journal-article","created":{"date-parts":[[2014,7,31]],"date-time":"2014-07-31T02:54:29Z","timestamp":1406775269000},"page":"3799-3801","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":22,"title":["Clinical Validation of the Lyra Direct HSV 1+2\/VZV Assay for Simultaneous Detection and Differentiation of Three Herpesviruses in Cutaneous and Mucocutaneous Lesions"],"prefix":"10.1128","volume":"52","author":[{"given":"Feinan","family":"Fan","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Futian Hospital, Guangdong Medical College, Shenzhen, China"},{"name":"Department of Laboratory Medicine, Memorial Sloan-Kettering Cancer Center, New York, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jeffrey","family":"Stiles","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Memorial Sloan-Kettering Cancer Center, New York, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albina","family":"Mikhlina","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Memorial Sloan-Kettering Cancer Center, New York, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xuedong","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Futian Hospital, Guangdong Medical College, Shenzhen, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. 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