{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:05:09Z","timestamp":1791299109826,"version":"4.1.0"},"reference-count":22,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Many studies demonstrate that delayed proper therapy in bloodstream infections caused by\n            <jats:named-content content-type=\"genus-species\">Staphylococcus aureus<\/jats:named-content>\n            increases the mortality rate, emphasizing the need to shorten the turnaround time for positive blood cultures. Different techniques are currently available, from phenotypic methods to more complex tests such as matrix-assisted laser desorption ionization\u2013time of flight mass spectrometry (MALDI-TOF), real-time PCR (RT-PCR), and fluorescence in situ hybridization using peptide nucleic acid probes (PNA FISH). This study evaluated the performance of the\n            <jats:named-content content-type=\"genus-species\">Staphylococcus<\/jats:named-content>\n            QuickFISH BC test (QFT), a novel FISH methodology, compared with the direct tube coagulase test (DTCT) on blood cultures exhibiting Gram-positive cocci in clusters. A total of 173 blood cultures collected from 128 different patients were analyzed using the DTCT, evaluated after both 4 and 24 h, and the QFT. A total of 179 isolates were identified using the Vitek2 system. Thirty-five out of 35\n            <jats:named-content content-type=\"genus-species\">Staphylococcus aureus<\/jats:named-content>\n            were correctly identified by the QFT (sensitivity = 100%), with a specificity of 100% (no green fluorescence was detected for strains different from\n            <jats:named-content content-type=\"genus-species\">S. aureus<\/jats:named-content>\n            ). The DTCT was positive after 4 h for 28 out of the 35 samples (sensitivity = 80%) and after 24 h for 31 out of the 35 samples (sensitivity = 88.57%). Among the remaining 144 isolates, one was then identified as\n            <jats:named-content content-type=\"genus-species\">Corynebacterium striatum<\/jats:named-content>\n            and two as\n            <jats:named-content content-type=\"genus-species\">Micrococcus luteus<\/jats:named-content>\n            . QFT identified 139 out of the 141 coagulase-negative staphylococci (CoNS) (sensitivity = 98.58%), showing again a specificity of 100% (no fluorescent red signals were detected for strains different from CoNS). We also discuss also the implementation process of this methodology in our setting, with particular emphasis on the workflow and the cost-effectiveness.\n          <\/jats:p>","DOI":"10.1128\/jcm.02103-12","type":"journal-article","created":{"date-parts":[[2012,10,25]],"date-time":"2012-10-25T04:29:53Z","timestamp":1351139393000},"page":"131-135","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Comparison of the Staphylococcus QuickFISH BC Test with the Tube Coagulase Test Performed on Positive Blood Cultures for Evaluation and Application in a Clinical Routine Setting"],"prefix":"10.1128","volume":"51","author":[{"given":"E.","family":"Carretto","sequence":"first","affiliation":[{"name":"Clinical Microbiology Laboratory, IRCCS Arcispedale Santa Maria Nuova, Reggio Emilia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Bardaro","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, IRCCS Arcispedale Santa Maria Nuova, Reggio Emilia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Russello","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, IRCCS Arcispedale Santa Maria Nuova, Reggio Emilia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Mirra","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, IRCCS Arcispedale Santa Maria Nuova, Reggio Emilia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Zuelli","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, IRCCS Arcispedale Santa Maria Nuova, Reggio Emilia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Barbarini","sequence":"additional","affiliation":[{"name":"Virology and Microbiology Laboratory, Fondazione IRCCS Policlinico San Matteo, Pavia, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/589717"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.298.15.1763"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.05022-11"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq373"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00627-10"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/660096"},{"key":"e_1_3_2_8_2","first-page":"1511","volume-title":"J. Med. Microbiol.","author":"Meex C","year":"2012","unstructured":"MeexC NeuvilleF DescyJ HuynenP HayetteMP De MolP MelinP 2012. Direct identification of bacteria from positive anaerobic BacT\/Alert(R) blood cultures by MALDI-TOF MS: MALDI Sepsityper(R) kit (Bruker) versus in-house saponin method for bacterial extraction. J. Med. Microbiol. 61: 1511\u2013 1516."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2012.01.010"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-012-1638-2"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01884-08"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00225-12"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00369-07"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.2.889-891.2003"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.1.247-251.2002"},{"key":"e_1_3_2_16_2","first-page":"30","article-title":"An evaluation of the AdvanDx Staphylococcus aureus\/CNS PNA FISH assay","volume":"22","author":"Hensley DM","year":"2009","unstructured":"HensleyDM TapiaR EncinaY . 2009. An evaluation of the AdvanDx Staphylococcus aureus\/CNS PNA FISH assay. Clin. Lab. Sci. 22: 30\u2013 33.","journal-title":"Clin. Lab. Sci."},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00623-08"},{"key":"e_1_3_2_18_2","volume-title":"Principles and procedures for blood cultures. Approved guideline. CLSI document M47-A","author":"CLSI","year":"2007","unstructured":"CLSI. 2007. Principles and procedures for blood cultures. Approved guideline. CLSI document M47-A. CLSI Wayne, PA."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2004.01052.x"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.15.5.848-851.1982"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01477-07"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2008.01966.x"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.2165\/11533020-000000000-00000"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02103-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02103-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:30:33Z","timestamp":1646479833000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02103-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1]]},"references-count":22,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,1]]}},"alternative-id":["10.1128\/JCM.02103-12"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02103-12","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,1]]},"assertion":[{"value":"2012-08-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-10-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-12-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}