{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T05:32:57Z","timestamp":1789018377181,"version":"build-2803163510"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2020,12,17]],"date-time":"2020-12-17T00:00:00Z","timestamp":1608163200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2020,12,17]],"date-time":"2020-12-17T00:00:00Z","timestamp":1608163200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100000272","name":"National Institute for Health Research","doi-asserted-by":"publisher","award":["16\/136\/46"],"award-info":[{"award-number":["16\/136\/46"]}],"id":[{"id":"10.13039\/501100000272","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000265","name":"UKRI | Medical Research Council","doi-asserted-by":"publisher","award":["MR\/N023129\/1"],"award-info":[{"award-number":["MR\/N023129\/1"]}],"id":[{"id":"10.13039\/501100000265","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000865","name":"Bill and Melinda Gates Foundation","doi-asserted-by":"publisher","award":["OPP117653"],"award-info":[{"award-number":["OPP117653"]}],"id":[{"id":"10.13039\/100000865","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100004440","name":"Wellcome","doi-asserted-by":"publisher","award":["106846\/Z\/15\/Z"],"award-info":[{"award-number":["106846\/Z\/15\/Z"]}],"id":[{"id":"10.13039\/100004440","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2020,12,17]]},"abstract":"<jats:p>Accurate assessment of the serotype distribution associated with pneumococcal colonization and disease is essential for evaluating and formulating pneumococcal vaccines and for informing vaccine policy. For this reason, we evaluated the concordance between pneumococcal serotyping results by latex agglutination, whole-genome sequencing (WGS) with PneumoCaT, and DNA microarray for samples from community carriage surveillance in Blantyre, Malawi. Nasopharyngeal swabs were collected according to WHO recommendations between 2015 and 2017 by using stratified random sampling among study populations.<\/jats:p>","DOI":"10.1128\/jcm.02103-20","type":"journal-article","created":{"date-parts":[[2020,10,20]],"date-time":"2020-10-20T09:46:06Z","timestamp":1603187166000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":15,"title":["Evaluation of Pneumococcal Serotyping of Nasopharyngeal-Carriage Isolates by Latex Agglutination, Whole-Genome Sequencing (PneumoCaT), and DNA Microarray in a High-Pneumococcal-Carriage-Prevalence Population in Malawi"],"prefix":"10.1128","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5285-7039","authenticated-orcid":false,"given":"Todd D.","family":"Swarthout","sequence":"first","affiliation":[{"name":"Malawi-Liverpool-Wellcome Trust Clinical Research Programme, Blantyre, Malawi"},{"name":"NIHR Global Health Research Unit on Mucosal Pathogens, Division of Infection and Immunity, University College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0931-0454","authenticated-orcid":false,"given":"Andrea","family":"Gori","sequence":"additional","affiliation":[{"name":"NIHR Global Health Research Unit on Mucosal Pathogens, Division of Infection and Immunity, University College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naor","family":"Bar-Zeev","sequence":"additional","affiliation":[{"name":"Malawi-Liverpool-Wellcome Trust Clinical Research Programme, Blantyre, Malawi"},{"name":"International Vaccine Access Center, Department of International Health, Johns Hopkins University, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0841-7586","authenticated-orcid":false,"given":"Arox W.","family":"Kamng\u2019ona","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences, College of Medicine, University of Malawi, Blantyre, Malawi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thandie S.","family":"Mwalukomo","sequence":"additional","affiliation":[{"name":"Department of Medicine, College of Medicine, University of Malawi, Blantyre, Malawi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farouck","family":"Bonomali","sequence":"additional","affiliation":[{"name":"Malawi-Liverpool-Wellcome Trust Clinical Research Programme, Blantyre, Malawi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roseline","family":"Nyirenda","sequence":"additional","affiliation":[{"name":"Malawi-Liverpool-Wellcome Trust Clinical Research Programme, Blantyre, Malawi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Comfort","family":"Brown","sequence":"additional","affiliation":[{"name":"Malawi-Liverpool-Wellcome Trust Clinical Research Programme, Blantyre, Malawi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacquline","family":"Msefula","sequence":"additional","affiliation":[{"name":"Malawi-Liverpool-Wellcome Trust Clinical Research Programme, Blantyre, Malawi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dean","family":"Everett","sequence":"additional","affiliation":[{"name":"The Queens Medical Research Institute, University of Edinburgh, Edinburgh, Scotland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charles","family":"Mwansambo","sequence":"additional","affiliation":[{"name":"Ministry of Health, Lilongwe, Malawi"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2528-9512","authenticated-orcid":false,"given":"Katherine","family":"Gould","sequence":"additional","affiliation":[{"name":"Institute for Infection and Immunity, St. George\u2019s, University of London, London, United Kingdom"},{"name":"BUGS Bioscience, London Bioscience Innovation Centre, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jason","family":"Hinds","sequence":"additional","affiliation":[{"name":"Institute for Infection and Immunity, St. George\u2019s, University of London, London, United Kingdom"},{"name":"BUGS Bioscience, London Bioscience Innovation Centre, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4573-449X","authenticated-orcid":false,"given":"Robert S.","family":"Heyderman","sequence":"additional","affiliation":[{"name":"Malawi-Liverpool-Wellcome Trust Clinical Research Programme, Blantyre, Malawi"},{"name":"NIHR Global Health Research Unit on Mucosal Pathogens, Division of Infection and Immunity, University College London, London, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil","family":"French","sequence":"additional","affiliation":[{"name":"Malawi-Liverpool-Wellcome Trust Clinical Research Programme, Blantyre, Malawi"},{"name":"Centre for Global Vaccine Research, Institute of Infection and Global Health, University of Liverpool, Liverpool, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1586\/erv.12.53"},{"key":"e_1_3_3_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S2214-109X(18)30247-X"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2013.04.077"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0027929"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.297.16.1784"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1001517"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(10)62225-8"},{"key":"e_1_3_3_9_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0103293"},{"key":"e_1_3_3_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02243-09"},{"key":"e_1_3_3_11_2","doi-asserted-by":"crossref","unstructured":"Swarthout TDMT Everett D Bar Zeev N Kamng\u2019ona A Chipasula T Malisita K Mwansambo C Banda M Hinds J Gould KA French N Heyderman RS. 2016. Persistent vaccine-type carriage of Streptococcus pneumoniae four years after introducing PCV13 in a 3+0 schedule in Malawi. Abstr Int Symp Pneumococci Pneumococcal Dis 2016 Glasgow Scotland.","DOI":"10.26226\/morressier.5731f0d4d462b8029237f7dd"},{"key":"e_1_3_3_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00157-11"},{"key":"e_1_3_3_13_2","first-page":"699","article-title":"The quellung reaction, a neglected microbiologic technique","volume":"43","author":"Austrian R","year":"1976","unstructured":"Austrian R. 1976. The quellung reaction, a neglected microbiologic technique. Mt Sinai J Med 43:699\u2013709.","journal-title":"Mt Sinai J Med"},{"key":"e_1_3_3_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.1001903"},{"key":"e_1_3_3_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-020-15786-9"},{"key":"e_1_3_3_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2013.08.062"},{"key":"e_1_3_3_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2105-12-88"},{"key":"e_1_3_3_18_2","unstructured":"Hinds JGK Witney AA Baldry SJ Lambertsen L Hannage WP Antonio M Turner P Newton R Wernisch L Aanensen DM Bentley S. 2009. Molecular serotyping of Streptococcus pneumoniae: a microarray-based tool with enhanced utility for isolate typing novel serotype discovery non-typeable investigation multiple carriage detection and direct analysis of nasopharyngeal swabs. Abstr 9th Eur Meet Mol Biol Pneumococcus (Europneumo) Bern Switzerland."},{"key":"e_1_3_3_19_2","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.2477"},{"key":"e_1_3_3_20_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.inf.0000048676.93549.d1"},{"key":"e_1_3_3_21_2","first-page":"501","article-title":"Multiple colonization of the upper respiratory tract of Papua New Guinea children with Haemophilus influenzae and Streptococcus pneumoniae","volume":"20","author":"Gratten M","year":"1989","unstructured":"Gratten M, Montgomery J, Gerega G, Gratten H, Siwi H, Poli A, Koki G. 1989. Multiple colonization of the upper respiratory tract of Papua New Guinea children with Haemophilus influenzae and Streptococcus pneumoniae. Southeast Asian J Trop Med Public Health 20:501\u2013509.","journal-title":"Southeast Asian J Trop Med Public Health"},{"key":"e_1_3_3_22_2","doi-asserted-by":"publisher","DOI":"10.1086\/528688"},{"key":"e_1_3_3_23_2","doi-asserted-by":"publisher","DOI":"10.1186\/1756-0500-6-49"},{"key":"e_1_3_3_24_2","doi-asserted-by":"publisher","DOI":"10.1099\/mgen.0.000186"},{"key":"e_1_3_3_25_2","doi-asserted-by":"publisher","DOI":"10.7717\/peerj.97"},{"key":"e_1_3_3_26_2","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2014.00258"},{"key":"e_1_3_3_27_2","unstructured":"Swarthout TD Gori A Bar-Zeev N Kamng\u2019ona AW Mwalukomo TS Bonomali F Nyirenda R Brown C Msefula J Everett D Mwansambo C Gould K Hinds J Heyderman RS French N. 2020. Study data and dictionary \u2014 evaluation of pneumococcal serotyping methods Blantyre Malawi 2015\u20132017. figshare doi:10.6084\/m9.figshare.12807872."}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02103-20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02103-20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T07:24:47Z","timestamp":1646465087000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02103-20"}},"subtitle":[],"editor":[{"given":"Daniel J.","family":"Diekema","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2020,12,17]]},"references-count":26,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2020,12,17]]}},"alternative-id":["10.1128\/JCM.02103-20"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02103-20","relation":{"has-preprint":[{"id-type":"doi","id":"10.1101\/2020.08.17.255224","asserted-by":"object"}]},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,17]]},"assertion":[{"value":"2020-08-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-10-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-12-17","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02103-20"}}