{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T17:48:08Z","timestamp":1760204888620,"version":"3.37.3"},"reference-count":13,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2017,4,1]],"date-time":"2017-04-01T00:00:00Z","timestamp":1491004800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100005869","name":"University of Fribourg","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100005869","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The rapid aminoglycoside NP (Nordmann\/Poirel) test was developed to rapidly identify multiple aminoglycoside (AG) resistance in\n            <jats:named-content content-type=\"genus-species\">Enterobacteriaceae<\/jats:named-content>\n            . It is based on the detection of the glucose metabolism related to enterobacterial growth in the presence of a defined concentration of amikacin plus gentamicin. Formation of acid metabolites was evidenced by a color change (orange to yellow) of the red phenol pH indicator. The rapid aminoglycoside NP test was evaluated by using bacterial colonies of 18 AG-resistant isolates producing 16S rRNA methylases, 20 AG-resistant isolates expressing AG-modifying enzymes (acetyl-, adenyl-, and phosphotransferases), and 10 isolates susceptible to AG. Its sensitivity and specificity were 100% and 97%, respectively, compared to the broth dilution method, which was taken as the gold standard for determining aminoglycoside resistance. The test is inexpensive, rapid (&lt;2 h), and implementable worldwide.\n          <\/jats:p>","DOI":"10.1128\/jcm.02107-16","type":"journal-article","created":{"date-parts":[[2017,1,19]],"date-time":"2017-01-19T02:20:35Z","timestamp":1484792435000},"page":"1074-1079","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":9,"title":["Rapid Aminoglycoside NP Test for Rapid Detection of Multiple Aminoglycoside Resistance in Enterobacteriaceae"],"prefix":"10.1128","volume":"55","author":[{"given":"Patrice","family":"Nordmann","sequence":"first","affiliation":[{"name":"Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, INSERM European Unit, LEA, IAME, Paris, France, and Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland"},{"name":"Institute of Microbiology, University Hospital Centre and University of Lausanne, Lausanne, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aur\u00e9lie","family":"Jayol","sequence":"additional","affiliation":[{"name":"Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, INSERM European Unit, LEA, IAME, Paris, France, and Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland"},{"name":"Laboratoire de Bact\u00e9riologie, CHU de Bordeaux, and CNRS UMR5234 Microbiologie Fondamentale et Pathog\u00e9nicit\u00e9, University of Bordeaux, Bordeaux, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan","family":"Dobias","sequence":"additional","affiliation":[{"name":"Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, INSERM European Unit, LEA, IAME, Paris, France, and Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laurent","family":"Poirel","sequence":"additional","affiliation":[{"name":"Emerging Antibiotic Resistance Unit, Medical and Molecular Microbiology, INSERM European Unit, LEA, IAME, Paris, France, and Department of Medicine, Faculty of Science, University of Fribourg, Fribourg, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1710.110655"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.2217\/fmb.11.49"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.drup.2010.08.003"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.idc.2016.02.011"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2011.08.016"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00926-07"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.66.1.24-26.1953"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid2111.150271"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-010-1004-1"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00869-16"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1586\/eri.12.25"},{"key":"e_1_3_2_13_2","volume-title":"Document M07-A9","author":"Clinical and Laboratory Standard Institute","year":"2012","unstructured":"Clinical and Laboratory Standard Institute. 2012. Methods for dilution of antimicrobial susceptibility tests for bacteria that grow aerobically. Approved standard, 9th ed. Document M07-A9. Clinical and Laboratory Standards Institute, Wayne, PA.","edition":"9"},{"key":"e_1_3_2_14_2","volume-title":"Document M100-S24","author":"Clinical and Laboratory Standard Institute","year":"2014","unstructured":"Clinical and Laboratory Standard Institute. 2014. Performance standards for antimicrobial susceptibility testing, 24th informational supplement. Document M100-S24. 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