{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T00:40:59Z","timestamp":1791852059690,"version":"4.3.4"},"reference-count":38,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            An accurate diagnosis of infection by\n            <jats:named-content content-type=\"genus-species\">Leishmania infantum<\/jats:named-content>\n            in dogs is fundamental for the control of zoonotic visceral leishmaniasis (VL). Histopathology (HP) and immunohistochemistry (IHC) are frequently used for the histological diagnosis of\n            <jats:named-content content-type=\"genus-species\">L. infantum<\/jats:named-content>\n            in dogs but have shown limited accuracy. To improve the sensitivity and specificity of the histological diagnosis of VL, we evaluated automated\n            <jats:italic>in situ<\/jats:italic>\n            hybridization (ISH) using a generic probe for\n            <jats:named-content content-type=\"genus-species\">Leishmania<\/jats:named-content>\n            and a specific probe for\n            <jats:named-content content-type=\"genus-species\">L. infantum<\/jats:named-content>\n            in surgical skin biopsy specimens of dogs. The ISH results were compared with those of HP and IHC, using parasitological culture as the reference standard. Skin samples from 51 dogs with cutaneous\n            <jats:named-content content-type=\"genus-species\">L. infantum<\/jats:named-content>\n            infection and 51 noninfected dogs were randomly selected from samples of dogs from various cities in Brazil where canine VL is endemic. These samples were processed for parasitological culture, HP, IHC, and ISH using both probes. The sensitivities of ISH using the specific probe, ISH using the generic probe, IHC, and HP were, respectively, 74.5%, 70.6%, 69.5%, and 57.6%. The specificity of both ISH probes tested was 100%, and there was no cross-hybridization of the generic and specific probes with selected pathogenic fungi and protozoa. The specific probe discriminated\n            <jats:named-content content-type=\"genus-species\">L. infantum<\/jats:named-content>\n            from the other species of\n            <jats:named-content content-type=\"genus-species\">Leishmania<\/jats:named-content>\n            that infect dogs in the New World. ISH is highly sensitive and specific for the diagnosis of\n            <jats:named-content content-type=\"genus-species\">L. infantum<\/jats:named-content>\n            in histologic samples of skin from infected dogs and can be used on routine biopsy material to make a diagnosis of leishmaniasis.\n          <\/jats:p>","DOI":"10.1128\/jcm.02123-12","type":"journal-article","created":{"date-parts":[[2012,11,8]],"date-time":"2012-11-08T07:26:25Z","timestamp":1352359585000},"page":"206-211","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":30,"title":["Sensitivity and Specificity of\n            <i>In Situ<\/i>\n            Hybridization for Diagnosis of Cutaneous Infection by Leishmania infantum in Dogs"],"prefix":"10.1128","volume":"51","author":[{"given":"Rodrigo C.","family":"Menezes","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa Cl\u00ednica em Dermatozoonoses em Animais Dom\u00e9sticos, Instituto de Pesquisa Cl\u00ednica Evandro Chagas, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiano B.","family":"Figueiredo","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa Cl\u00ednica em Dermatozoonoses em Animais Dom\u00e9sticos, Instituto de Pesquisa Cl\u00ednica Evandro Chagas, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Annabel G.","family":"Wise","sequence":"additional","affiliation":[{"name":"Diagnostic Center for Population and Animal Health, College of Veterinary Medicine, Michigan State University, Lansing, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria F.","family":"Madeira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Vigil\u00e2ncia em Leishmanioses, Instituto de Pesquisa Cl\u00ednica Evandro Chagas, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raquel V. C.","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Epidemiologia Cl\u00ednica, Instituto de Pesquisa Cl\u00ednica Evandro Chagas, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T\u00e2nia M. P.","family":"Schubach","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Pesquisa Cl\u00ednica em Dermatozoonoses em Animais Dom\u00e9sticos, Instituto de Pesquisa Cl\u00ednica Evandro Chagas, Funda\u00e7\u00e3o Oswaldo Cruz, Rio de Janeiro, Rio de Janeiro, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matti","family":"Kiupel","sequence":"additional","affiliation":[{"name":"Diagnostic Center for Population and Animal Health, College of Veterinary Medicine, Michigan State University, Lansing, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingeborg M.","family":"Langohr","sequence":"additional","affiliation":[{"name":"Diagnostic Center for Population and Animal Health, College of Veterinary Medicine, Michigan State University, Lansing, Michigan, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","volume-title":"Control of the leishmaniases: report of a meeting of the WHO Expert Committee on the Control of Leishmaniases, Geneva, 22-26 March 2010. 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