{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T03:57:07Z","timestamp":1771991827058,"version":"3.50.1"},"reference-count":28,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2021,1,21]],"date-time":"2021-01-21T00:00:00Z","timestamp":1611187200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2021,1,21]]},"abstract":"<jats:p>\n            Oropharyngeal\n            <jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>\n            (CT) infections and, especially,\n            <jats:named-content content-type=\"genus-species\">Neisseria gonorrhoeae<\/jats:named-content>\n            (NG) infections are common, but few commercial nucleic acid amplification tests (NAATs) specify extragenital samples for intended use. The test characteristics of the cobas 4800 CT\/NG assay were evaluated for oropharyngeal swabs. The technical validation included analysis of the specificity, sensitivity, dynamic range, linearity, efficiency, and precision. The probability of detection curve combined with historical data enabled the estimation of potentially missed diagnoses.\n          <\/jats:p>","DOI":"10.1128\/jcm.02137-20","type":"journal-article","created":{"date-parts":[[2020,11,4]],"date-time":"2020-11-04T14:26:38Z","timestamp":1604499998000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":7,"title":["Despite Excellent Test Characteristics of the cobas 4800 CT\/NG Assay, Detection of Oropharyngeal Chlamydia trachomatis and Neisseria gonorrhoeae Remains Challenging"],"prefix":"10.1128","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-3611-7732","authenticated-orcid":false,"given":"J. M.","family":"van Niekerk","sequence":"first","affiliation":[{"name":"Department of Medical Microbiology, Care and Public Health Research Institute (CAPHRI), Maastricht University Medical Centre (MUMC+), Maastricht, the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8837-8283","authenticated-orcid":false,"given":"B. M. J. W.","family":"van der Veer","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Care and Public Health Research Institute (CAPHRI), Maastricht University Medical Centre (MUMC+), Maastricht, the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C. J. P. A.","family":"Hoebe","sequence":"additional","affiliation":[{"name":"Department of Sexual Health, Infectious Diseases and Environmental Health, South Limburg Public Health Service, Heerlen, the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"van de Bovenkamp","sequence":"additional","affiliation":[{"name":"Laboratory for Medical Microbiology, PAMM, Veldhoven, the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"van Herk","sequence":"additional","affiliation":[{"name":"Laboratory for Medical Microbiology, PAMM, Veldhoven, the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I. H. M.","family":"van Loo","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Care and Public Health Research Institute (CAPHRI), Maastricht University Medical Centre (MUMC+), Maastricht, the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. B.","family":"van Alphen","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Care and Public Health Research Institute (CAPHRI), Maastricht University Medical Centre (MUMC+), Maastricht, the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5326-3985","authenticated-orcid":false,"given":"P. F. G.","family":"Wolffs","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Care and Public Health Research Institute (CAPHRI), Maastricht University Medical Centre (MUMC+), Maastricht, the Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.18.228486"},{"key":"e_1_3_1_3_2","volume-title":"Emergence of multi-drug resistant Neisseria gonorrhoeae - Threat of global rise in untreatable sexually transmitted infections Fact sheet","author":"World Health Organization, Department of Reproductive Health and Research","year":"2011","unstructured":"World Health Organization, Department of Reproductive Health and Research. 2011. Emergence of multi-drug resistant Neisseria gonorrhoeae - Threat of global rise in untreatable sexually transmitted infections Fact sheet. World Health Organization, Geneva, Switzerland."},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1177\/0956462415618837"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1177\/0956462412472837"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/430704"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu184"},{"key":"e_1_3_1_8_2","first-page":"1","article-title":"Recommendations for the laboratory-based detection of Chlamydia trachomatis and Neisseria gonorrhoeae\u20132014","volume":"63","author":"Centers for Disease Control and Prevention","year":"2014","unstructured":"Centers for Disease Control and Prevention. 2014. Recommendations for the laboratory-based detection of Chlamydia trachomatis and Neisseria gonorrhoeae\u20132014. MMWR Recomm Rep 63:1\u201319.","journal-title":"MMWR Recomm Rep"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01217-11"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0120979"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.41.2.835-837.2003"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01171-20"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/jiz668"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1556\/1886.2017.00018"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2010.042812"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e31824e26ff"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2012.08.004"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2012-050535"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.1556\/EuJMI.2.2012.0001"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2013.06.020"},{"key":"e_1_3_1_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0165920"},{"key":"e_1_3_1_22_2","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2018.23.50.1800253"},{"key":"e_1_3_1_23_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2008.112797"},{"key":"e_1_3_1_24_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2009.040634"},{"key":"e_1_3_1_25_2","doi-asserted-by":"publisher","DOI":"10.1136\/sextrans-2019-054002"},{"key":"e_1_3_1_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01634-10"},{"key":"e_1_3_1_27_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02631-12"},{"key":"e_1_3_1_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06481-11"},{"key":"e_1_3_1_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03552-13"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02137-20","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02137-20","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T10:54:25Z","timestamp":1646477665000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02137-20"}},"subtitle":[],"editor":[{"given":"Erik","family":"Munson","sequence":"additional","affiliation":[],"role":[{"role":"editor","vocabulary":"crossref"}]}],"short-title":[],"issued":{"date-parts":[[2021,1,21]]},"references-count":28,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2021,1,21]]}},"alternative-id":["10.1128\/JCM.02137-20"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02137-20","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,1,21]]},"assertion":[{"value":"2020-08-18","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2020-11-01","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-01-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e02137-20"}}