{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:31:29Z","timestamp":1787016689544,"version":"build-2736575974"},"reference-count":44,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2017,2,22]],"date-time":"2017-02-22T00:00:00Z","timestamp":1487721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Three multistate outbreaks between 2014 and 2016, involving case patients in and outside the United States, were linked to stone fruit, caramel apples, and packaged leafy green salad contaminated with\n                    <jats:named-content content-type=\"genus-species\">Listeria monocytogenes<\/jats:named-content>\n                    singleton sequence type 382 (ST382), a serotype IVb-v1 clone with limited genomic divergence. Isolates from these outbreaks and other ST382 isolates not associated with these outbreaks were analyzed by whole-genome sequencing (WGS) analysis. The primary differences among ST382 strains were single nucleotide polymorphisms (SNPs). WGS analysis differentiated ST382 from a clonal complex 1 outbreak strain co-contaminating the caramel apples. WGS clustered food, environmental, and clinical isolates within each outbreak, and also differentiated among the three outbreak strains and epidemiologically unrelated ST382 isolates, which were indistinguishable by pulsed-field gel electrophoresis. ST382 appeared to be an emerging clone that began to diverge from its ancestor approximately 32 years before 2016. We estimated that there was 1.29 nucleotide substitution per genome (2.94 Mbp) per year for this clone.\n                  <\/jats:p>","DOI":"10.1128\/jcm.02140-16","type":"journal-article","created":{"date-parts":[[2017,1,4]],"date-time":"2017-01-04T21:40:28Z","timestamp":1483566028000},"page":"931-941","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":53,"title":["Singleton Sequence Type 382, an Emerging Clonal Group of Listeria monocytogenes Associated with Three Multistate Outbreaks Linked to Contaminated Stone Fruit, Caramel Apples, and Leafy Green Salad"],"prefix":"10.1128","volume":"55","author":[{"given":"Yi","family":"Chen","sequence":"first","affiliation":[{"name":"Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Luo","sequence":"additional","affiliation":[{"name":"Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James","family":"Pettengill","sequence":"additional","affiliation":[{"name":"Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ruth","family":"Timme","sequence":"additional","affiliation":[{"name":"Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David","family":"Melka","sequence":"additional","affiliation":[{"name":"Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew","family":"Doyle","sequence":"additional","affiliation":[{"name":"Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alikeh","family":"Jackson","sequence":"additional","affiliation":[{"name":"Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mickey","family":"Parish","sequence":"additional","affiliation":[{"name":"Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas S.","family":"Hammack","sequence":"additional","affiliation":[{"name":"Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc W.","family":"Allard","sequence":"additional","affiliation":[{"name":"Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric W.","family":"Brown","sequence":"additional","affiliation":[{"name":"Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Errol A.","family":"Strain","sequence":"additional","affiliation":[{"name":"Center for Food Safety and Applied Nutrition, Food and Drug Administration, College Park, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01486-16"},{"key":"e_1_3_3_3_2","first-page":"282","article-title":"Notes from the field: listeriosis associated with stone fruit\u2014United States, 2014","volume":"64","author":"Jackson BR","year":"2015","unstructured":"Jackson BR Salter M Tarr C Conrad A Harvey E Steinbock L Saupe A Sorenson A Katz L Stroika S Jackson KA Carleton H Kucerova Z Melka D Strain E Parish M Mody RK , Centers for Disease Control and Prevention (CDC). 2015. Notes from the field: listeriosis associated with stone fruit\u2014United States, 2014. MMWR Morb Mortal Wkly Rep64:282\u2013283.","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"e_1_3_3_4_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268816003083"},{"key":"e_1_3_3_5_2","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.mm6533a6"},{"key":"e_1_3_3_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw359"},{"key":"e_1_3_3_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw242"},{"key":"e_1_3_3_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00081-16"},{"key":"e_1_3_3_9_2","unstructured":"Centers for Disease Control and Prevention. 2016. Multistate outbreak of listeriosis linked to packaged salads produced at Springfield Ohio Dole processing facility (final update). http:\/\/www.cdc.gov\/listeria\/outbreaks\/bagged-salads-01-16\/index.html\n. 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Genetic algorithm approaches for the phylogenetic analysis of large biological sequence datasets under the maximum likelihood criterion. PhD dissertation. The University of Texas at Austin, Austin, TX."},{"key":"e_1_3_3_42_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2148-7-214"},{"key":"e_1_3_3_43_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF02101694"},{"key":"e_1_3_3_44_2","doi-asserted-by":"publisher","DOI":"10.1093\/molbev\/mss084"},{"key":"e_1_3_3_45_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gkw387"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02140-16","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02140-16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T05:08:23Z","timestamp":1646456903000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02140-16"}},"subtitle":[],"editor":[{"given":"Daniel J.","family":"Diekema","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,3]]},"references-count":44,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,3]]}},"alternative-id":["10.1128\/JCM.02140-16"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02140-16","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,3]]},"assertion":[{"value":"2016-10-22","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-12-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-02-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}