{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,21]],"date-time":"2026-03-21T19:11:44Z","timestamp":1774120304814,"version":"3.50.1"},"reference-count":26,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2013,2,1]],"date-time":"2013-02-01T00:00:00Z","timestamp":1359676800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Interest in lateral-flow devices (LFDs) as potential point-of-care assays for the diagnosis of infectious diseases has increased. Our objective was to evaluate the interlaboratory and interstudy reproducibility and the effects of antifungal therapy on an LFD developed for invasive pulmonary aspergillosis (IPA) detection. An established neutropenic guinea pig model of IPA caused by\n            <jats:named-content content-type=\"genus-species\">Aspergillus fumigatus<\/jats:named-content>\n            was used. At predetermined time points (1 h and 3, 5, and 7 days postinoculation), blood and bronchoalveolar lavage (BAL) fluid were collected from infected and uninfected animals. In a separate experiment, guinea pigs were treated with posaconazole (10 mg\/kg of body weight orally [p.o.] twice a day [BID]), voriconazole (10 mg\/kg p.o. BID), liposomal amphotericin B (10 mg\/kg intraperitoneally [i.p.] once a day [QD]), or caspofungin (2 mg\/kg i.p. QD), and samples were collected on days 7 and 11. Each laboratory independently evaluated the IgG monoclonal antibody-based LFD. Galactomannan and (1\u21923)-\u03b2-\n            <jats:sc>d<\/jats:sc>\n            -glucan were also measured using commercially available kits. Good interlaboratory agreement was observed with the LFD, as the results for 97% (32\/33) of the serum and 78.8% (26\/33) of the BAL fluid samples from infected animals were in agreement. Good interstudy agreement was also observed. The serum sensitivity of each surrogate-marker assay was reduced in animals treated with antifungals. In contrast, these markers remained elevated within the BAL fluids of treated animals, which was consistent with the fungal burden and histopathology results. These results demonstrate that the LFD assay is reproducible between different laboratories and studies. However, the sensitivity of this assay and other markers of IPA may be reduced with serum in the presence of antifungal therapy.\n          <\/jats:p>","DOI":"10.1128\/jcm.02142-12","type":"journal-article","created":{"date-parts":[[2012,11,23]],"date-time":"2012-11-23T03:50:19Z","timestamp":1353642619000},"page":"459-465","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":55,"title":["Interlaboratory and Interstudy Reproducibility of a Novel Lateral-Flow Device and Influence of Antifungal Therapy on Detection of Invasive Pulmonary Aspergillosis"],"prefix":"10.1128","volume":"51","author":[{"given":"Nathan P.","family":"Wiederhold","sequence":"first","affiliation":[{"name":"University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA"},{"name":"University of Texas at Austin College of Pharmacy, Austin, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laura K.","family":"Najvar","sequence":"additional","affiliation":[{"name":"University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA"},{"name":"South Texas Veterans Health Care System, San Antonio, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosie","family":"Bocanegra","sequence":"additional","affiliation":[{"name":"University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA"},{"name":"South Texas Veterans Health Care System, San Antonio, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William R.","family":"Kirkpatrick","sequence":"additional","affiliation":[{"name":"University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA"},{"name":"South Texas Veterans Health Care System, San Antonio, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas F.","family":"Patterson","sequence":"additional","affiliation":[{"name":"University of Texas Health Science Center at San Antonio, San Antonio, Texas, USA"},{"name":"South Texas Veterans Health Care System, San Antonio, Texas, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher R.","family":"Thornton","sequence":"additional","affiliation":[{"name":"University of Exeter, Exeter, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/318483"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1997.15.1.139"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/504810"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/509917"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/525258"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1086\/429921"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.2011.090464"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1086\/527382"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.contraception.2004.03.012"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cir613"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1097\/00042560-200105010-00011"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.7.3935-3941.2005"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.8.1.166-169.2001"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00068-08"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00268-09"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jinf.2012.10.003"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.3791\/3721"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00276-08"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.5.1908-1911.2004"},{"key":"e_1_3_2_21_2","volume-title":"Guide for the care and use of laboratory animals.","author":"National Research Council","year":"1996","unstructured":"National Research Council. 1996. Guide for the care and use of laboratory animals. National Academies Press, Washington, DC."},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00787-06"},{"key":"e_1_3_2_23_2","volume-title":"Assay for (1,3)-beta-d-glucan in serum.","author":"Associates of Cape Cod Inc","year":"2008","unstructured":"Associates of Cape Cod Inc. 2008. Assay for (1,3)-beta-d-glucan in serum. Associates of Cape Cod Inc, East Falmouth, MA."},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/EC.4.7.1308-1316.2005"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.2.497-500.1995"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.60.6.2237-2245.1992"},{"key":"e_1_3_2_27_2","volume-title":"Abstr. 51st Intersci. Conf. Antimicrob. Agents Chemother.","author":"Wiederhold NP","year":"2011","unstructured":"WiederholdNP ThorntonCR. 2011. Lack of cross-reactivity and interference of commonly used intravenous antimicrobials with a lateral-flow device for the diagnosis of invasive aspergillosis, abstr M-1538. Abstr. 51st Intersci. Conf. Antimicrob. Agents Chemother., Chicago, IL. American Society for Microbiology, Washington, DC."}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02142-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02142-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:22:51Z","timestamp":1646479371000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02142-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,2]]},"references-count":26,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2013,2]]}},"alternative-id":["10.1128\/JCM.02142-12"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02142-12","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,2]]},"assertion":[{"value":"2012-08-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-11-15","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-01-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}