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The purpose of this study was to correlate the clinicopathological findings and viral antigen distribution with the genotypic characteristics of CCoV in 11 puppies from nine premises in five states that were submitted for diagnostic investigation at Cornell University between 2008 and 2013. CCoV antigen was found in epithelial cells of small intestinal villi in all puppies and the colon in 2 of the 10 puppies where colon specimens were available. No evidence of systemic CCoV infection was found. Comparative sequence analyses of viral RNA extracted from intestinal tissues revealed CCoV-II genotype in 9 out of 11 puppies. Of the nine CCoV-IIs, five were subtyped as group IIa and one as IIb, while three CCoVs could not be subtyped. One of the CCoV-IIa variants was isolated in cell culture. Infection with CCoV alone was found in five puppies, of which two also had small intestinal intussusception. Concurrent infections with either parvovirus (<jats:italic>n<\/jats:italic>= 1), attaching-effacing<jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>(<jats:italic>n<\/jats:italic>= 4), or protozoan parasites (<jats:italic>n<\/jats:italic>= 3) were found in the other six puppies. CCoV is an important differential diagnosis in outbreaks of severe enterocolitis among puppies between 4 days and 21 weeks of age that are housed at high population density. These findings will assist with the rapid laboratory diagnosis of enteritis in puppies and highlight the need for continued surveillance for CCoV variants and intestinal viral diseases of global significance.<\/jats:p>","DOI":"10.1128\/jcm.02158-14","type":"journal-article","created":{"date-parts":[[2014,9,25]],"date-time":"2014-09-25T02:30:32Z","timestamp":1411612232000},"page":"4230-4238","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Genotypic Characterization of Canine Coronaviruses Associated with Fatal Canine Neonatal Enteritis in the United States"],"prefix":"10.1128","volume":"52","author":[{"given":"Beth N.","family":"Licitra","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary R.","family":"Whittaker","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Edward J.","family":"Dubovi","sequence":"additional","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA"},{"name":"Animal Health Diagnostic Center, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerald E.","family":"Duhamel","sequence":"additional","affiliation":[{"name":"Department of Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA"},{"name":"Department of Biomedical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA"},{"name":"Animal Health Diagnostic Center, College of Veterinary Medicine, Cornell University, Ithaca, New York, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"359","volume-title":"Proceedings of the 18th annual meeting of the United States Animal Health Association. 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