{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T13:16:44Z","timestamp":1762867004488,"version":"3.37.3"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2017,2,22]],"date-time":"2017-02-22T00:00:00Z","timestamp":1487721600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/501100003549","name":"Hungarian Scientific Research Fund","doi-asserted-by":"crossref","award":["OTKA 112826"],"award-info":[{"award-number":["OTKA 112826"]}],"id":[{"id":"10.13039\/501100003549","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100000268","name":"RCUK | Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/M023052\/1","BB\/G018553\/1","BB\/L024209\/1"],"award-info":[{"award-number":["BB\/M023052\/1","BB\/G018553\/1","BB\/L024209\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            <jats:named-content content-type=\"genus-species\">Actinobacillus pleuropneumoniae<\/jats:named-content>\n            causes pleuropneumonia, an economically significant lung disease of pigs. Recently, isolates of\n            <jats:named-content content-type=\"genus-species\">A. pleuropneumoniae<\/jats:named-content>\n            that were serologically distinct from the previously characterized 15 serovars were described, and a proposal was put forward that they comprised a new serovar, serovar 16. Here we used whole-genome sequencing of the proposed serovar 16 reference strain A-85\/14 to confirm the presence of a unique capsular polysaccharide biosynthetic locus. For molecular diagnostics, primers were designed from the capsule locus of strain A-85\/14, and a PCR was formulated that differentiated serovar 16 isolates from all 15 known serovars and other common respiratory pathogenic\/commensal bacteria of pigs. Analysis of the capsule locus of strain A-85\/14 combined with the previous serological data show the existence of a sixteenth serovar\u2014designated serovar 16\u2014of\n            <jats:named-content content-type=\"genus-species\">A. pleuropneumoniae<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/jcm.02166-16","type":"journal-article","created":{"date-parts":[[2017,1,5]],"date-time":"2017-01-05T02:40:28Z","timestamp":1483584028000},"page":"902-907","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":39,"title":["A Unique Capsule Locus in the Newly Designated Actinobacillus pleuropneumoniae Serovar 16 and Development of a Diagnostic PCR Assay"],"prefix":"10.1128","volume":"55","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8491-4779","authenticated-orcid":false,"given":"Janine T.","family":"Boss\u00e9","sequence":"first","affiliation":[{"name":"Section of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yanwen","family":"Li","sequence":"additional","affiliation":[{"name":"Section of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rita","family":"S\u00e1rk\u00f6zi","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marcelo","family":"Gottschalk","sequence":"additional","affiliation":[{"name":"Groupe de Recherche sur les Maladies Infectieuses du Porc, Facult\u00e9 de M\u00e9decine V\u00e9t\u00e9rinaire, Universit\u00e9 de Montr\u00e9al, Montr\u00e9al, Qu\u00e9bec, Canada"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"\u00d8ystein","family":"Angen","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infection Control, Statens Serum Institut, Copenhagen, Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katerina","family":"Nedbalcova","sequence":"additional","affiliation":[{"name":"Veterinary Research Institute, Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrew N.","family":"Rycroft","sequence":"additional","affiliation":[{"name":"Department of Pathology and Pathogen Biology, The Royal Veterinary College, Hawkshead Campus, Hatfield, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00e1szl\u00f3","family":"Fodor","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, University of Veterinary Medicine, Budapest, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6368-4724","authenticated-orcid":false,"given":"Paul R.","family":"Langford","sequence":"additional","affiliation":[{"name":"Section of Paediatrics, Department of Medicine, Imperial College London, St. Mary's Campus, London, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1286-4579(01)01534-9"},{"key":"e_1_3_2_3_2","first-page":"653","volume-title":"Diseases of swine","author":"Gottschalk M","year":"2012","unstructured":"Gottschalk M . 2012. 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