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We describe the design, validation, and implementation of a novel single nucleotide polymorphism (SNP)-based typing platform that offers a rapid, reliable, and robust tool to achieve this with improved diagnostic accuracy compared to existing molecular tests. Furthermore, the assays described are designed such that they supplement, and can be run as an intrinsic part of, a previously described assay identifying<jats:italic>Brucella<\/jats:italic>isolates to the species level (K. K. Gopaul, C. J. Smith, M. S. Koylass, and A. M. Whatmore, BMC Microbiol. 8:86), giving a comprehensive molecular typing platform.<\/jats:p>","DOI":"10.1128\/jcm.02193-09","type":"journal-article","created":{"date-parts":[[2010,2,25]],"date-time":"2010-02-25T05:30:02Z","timestamp":1267075802000},"page":"1461-1464","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":33,"title":["Rapid and Reliable Single Nucleotide Polymorphism-Based Differentiation of<i>Brucella<\/i>Live Vaccine Strains from Field Strains"],"prefix":"10.1128","volume":"48","author":[{"given":"Krishna K.","family":"Gopaul","sequence":"first","affiliation":[{"name":"Department of Statutory and Exotic Bacterial Disease, Veterinary Laboratories Agency, Woodham Lane, New Haw, United Kingdom KT15 3NB"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jessica","family":"Sells","sequence":"additional","affiliation":[{"name":"Department of Statutory and Exotic Bacterial Disease, Veterinary Laboratories Agency, Woodham Lane, New Haw, United Kingdom KT15 3NB"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Betsy J.","family":"Bricker","sequence":"additional","affiliation":[{"name":"Bacterial Diseases of Livestock, United States Department of Agriculture, 2300N Daytona Avenue, Ames, Iowa 50010-000"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oswald R.","family":"Crasta","sequence":"additional","affiliation":[{"name":"Virginia Bioinformatics Institute, Washington Street, MC 0477, Blacksburg, Virginia 24061"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adrian M.","family":"Whatmore","sequence":"additional","affiliation":[{"name":"Department of Statutory and Exotic Bacterial Disease, Veterinary Laboratories Agency, Woodham Lane, New Haw, United Kingdom KT15 3NB"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"40","volume":"3","year":"2001","unstructured":"Acha, P. 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