{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,11,8]],"date-time":"2026-11-08T03:43:33Z","timestamp":1794109413333,"version":"4.8.0"},"reference-count":47,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2011,3,1]],"date-time":"2011-03-01T00:00:00Z","timestamp":1298937600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    To estimate the prevalence and public health significance of cryptosporidiosis in preweaned calves in China, 801 fecal samples from eight farms in seven areas in Henan Province were examined for\n                    <jats:named-content content-type=\"genus-species\">Cryptosporidium<\/jats:named-content>\n                    oocysts. The overall infection rate of\n                    <jats:named-content content-type=\"genus-species\">Cryptosporidium<\/jats:named-content>\n                    was 21.5%, with the farm in Xinxiang having the highest prevalence (40%). No significant difference in infection rates was observed between seasons.\n                    <jats:named-content content-type=\"genus-species\">Cryptosporidium<\/jats:named-content>\n                    spp. were characterized by PCR-restriction fragment length polymorphism (RFLP) analysis of the small subunit (SSU) rRNA gene and DNA sequencing of the 60-kDa glycoprotein (gp60) gene. The SSU rRNA-based PCR identified four\n                    <jats:named-content content-type=\"genus-species\">Cryptosporidium<\/jats:named-content>\n                    species, including\n                    <jats:named-content content-type=\"genus-species\">Cryptosporidium parvum<\/jats:named-content>\n                    (54\/172),\n                    <jats:named-content content-type=\"genus-species\">C. bovis<\/jats:named-content>\n                    (65\/172),\n                    <jats:named-content content-type=\"genus-species\">C. ryanae<\/jats:named-content>\n                    (19\/172), and\n                    <jats:named-content content-type=\"genus-species\">C. andersoni<\/jats:named-content>\n                    (12\/172), and the occurrence of infections with mixed species (22\/172). The earliest detection of\n                    <jats:named-content content-type=\"genus-species\">C. bovis<\/jats:named-content>\n                    was in calves of 1 week of age, showing that the prepatent period was shorter than the previously stated 10 to 12 days. Infections with\n                    <jats:named-content content-type=\"genus-species\">C. parvum<\/jats:named-content>\n                    peaked in summer, whereas\n                    <jats:named-content content-type=\"genus-species\">C. bovis<\/jats:named-content>\n                    dominated in autumn and winter. There was no apparent difference in the age of cattle infected with either\n                    <jats:named-content content-type=\"genus-species\">C. parvum<\/jats:named-content>\n                    or\n                    <jats:named-content content-type=\"genus-species\">C. bovis<\/jats:named-content>\n                    . Sequencing analysis of the gp60 gene showed all 67\n                    <jats:named-content content-type=\"genus-species\">C. parvum<\/jats:named-content>\n                    samples belonged to subtype IIdA19G1. These findings suggested that the transmission of\n                    <jats:named-content content-type=\"genus-species\">Cryptosporidium<\/jats:named-content>\n                    spp. in preweaned calves in Henan, China, appeared to be different from other areas both at genotype and subtype levels. Further molecular epidemiologic studies (including samples from both calves and humans) are needed to elucidate the transmission dynamics and public significance of\n                    <jats:named-content content-type=\"genus-species\">C. parvum<\/jats:named-content>\n                    in cattle in China.\n                  <\/jats:p>","DOI":"10.1128\/jcm.02194-10","type":"journal-article","created":{"date-parts":[[2010,12,22]],"date-time":"2010-12-22T22:29:27Z","timestamp":1293056967000},"page":"1077-1082","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":119,"title":["Characteristics of Cryptosporidium Transmission in Preweaned Dairy Cattle in Henan, China"],"prefix":"10.1128","volume":"49","author":[{"given":"Rongjun","family":"Wang","sequence":"first","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helei","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanru","family":"Sun","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Longxian","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fuchun","family":"Jian","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng","family":"Qi","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changshen","family":"Ning","sequence":"additional","affiliation":[{"name":"College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lihua","family":"Xiao","sequence":"additional","affiliation":[{"name":"Division of Foodborne, Waterborne, and Environmental Diseases, National Center for Emerging and Zoonotic Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-006-0140-0"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.6.2744-2747.2003"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2010.01.017"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2008.02.029"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2007.10.023"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetpar.2008.10.096"},{"key":"e_1_3_2_8_2","first-page":"1","article-title":"Genotype and subtype analysis of Cryptosporidium isolates from calves and lambs in Galicia (NW Spain)","volume":"12","author":"D\u00edaz P.","year":"2010","unstructured":"D\u00edazP.. 2010. 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