{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T15:30:20Z","timestamp":1775057420762,"version":"3.50.1"},"reference-count":24,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2013,12,1]],"date-time":"2013-12-01T00:00:00Z","timestamp":1385856000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,12]]},"abstract":"ABSTRACT<\/jats:title>\n \n Here, we compare the sensitivities and times to detection (TTD) of BacT\/Alert Pediatric FAN (PF) and Bactec Peds Plus blood culture bottles. Test bottles were inoculated with 2 ml of banked whole blood, 1-ml aliquots of antibiotic suspension, and organisms diluted to simulate a bacteremia level of 10 to 100 CFU\/ml. The control bottles were inoculated with 3 ml of banked blood and organism suspensions only. The organism-drug combinations were\n Staphylococcus epidermidis<\/jats:named-content>\n and vancomycin, methicillin-resistant\n Staphylococcus aureus<\/jats:named-content>\n and vancomycin,\n Streptococcus pneumoniae<\/jats:named-content>\n , vancomycin, and ceftriaxone,\n Streptococcus agalactiae<\/jats:named-content>\n , ampicillin, and cefotaxime,\n Escherichia coli<\/jats:named-content>\n , cefotaxime, and cefepime,\n Pseudomonas aeruginosa<\/jats:named-content>\n , piperacillin-tazobactam, cefepime, and gentamicin,\n Neisseria meningitidis<\/jats:named-content>\n and ceftriaxone, and\n Haemophilus influenzae<\/jats:named-content>\n and ceftriaxone. The control and test bottle combinations were tested in duplicate. The bottles were incubated for 5 days; 32 control and 104 test bottles were incubated. Overall, the bacterial recovery rates for the PF and Peds Plus bottles were 37% and 62%, 94% and 100% in the controls, 19% and 50% in the test bottles, and 33% and 92% in the bottles with vancomycin, respectively. No bacteria were recovered from the bottles with\n S. pneumoniae<\/jats:named-content>\n ,\n S. agalactiae<\/jats:named-content>\n ,\n E. coli<\/jats:named-content>\n ,\n N. meningitidis<\/jats:named-content>\n , or\n H. influenzae<\/jats:named-content>\n in combination with cefotaxime or ceftriaxone. The Peds Plus system detected\n P. aeruginosa<\/jats:named-content>\n in bottles with cefepime and piperacillin-tazobactam, but the PF system recovered bacteria only in bottles with trough levels of piperacillin-tazobactam. The mean TTD were shorter in the Peds Plus system controls (14.2 versus 18.0 h;\n P<\/jats:italic>\n = 0.001) and the test bottles (14.3 versus 17.8 h;\n P<\/jats:italic>\n = 0.008) than in the PF bottles. Overall, we demonstrated superior sensitivity, TTD, and antibiotic neutralization in the Bactec Peds Plus system compared to those in the Pediatric FAN system.\n <\/jats:p>","DOI":"10.1128\/jcm.02205-13","type":"journal-article","created":{"date-parts":[[2013,10,3]],"date-time":"2013-10-03T01:00:40Z","timestamp":1380762040000},"page":"4083-4086","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Superior Sensitivity and Decreased Time to Detection with the Bactec Peds Plus\/F System Compared to the BacT\/Alert Pediatric FAN Blood Culture System"],"prefix":"10.1128","volume":"51","author":[{"given":"K. V.","family":"Sullivan","sequence":"first","affiliation":[{"name":"The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA"},{"name":"Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. N.","family":"Turner","sequence":"additional","affiliation":[{"name":"The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. P.","family":"Lancaster","sequence":"additional","affiliation":[{"name":"The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. R.","family":"Shah","sequence":"additional","affiliation":[{"name":"Philadelphia VA Medical Center, Philadelphia, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L. J.","family":"Chandler","sequence":"additional","affiliation":[{"name":"Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA"},{"name":"Philadelphia VA Medical Center, Philadelphia, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. F.","family":"Friedman","sequence":"additional","affiliation":[{"name":"The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA"},{"name":"Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. L.","family":"Blecker-Shelly","sequence":"additional","affiliation":[{"name":"The Children's Hospital of Philadelphia, Philadelphia, Pennsylvania, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"999","article-title":"Blood culture quality improvement: a College of American Pathologists Q-Probes study involving 909 institutions and 289 572 blood culture sets","volume":"120","author":"Schifman RB","year":"1996","unstructured":"SchifmanRBBachnerPHowanitzPJ. 1996. Blood culture quality improvement: a College of American Pathologists Q-Probes study involving 909 institutions and 289 572 blood culture sets. Arch. Pathol. Lab. Med. 120:999\u20131002.","journal-title":"Arch. Pathol. Lab. Med."},{"key":"e_1_3_2_3_2","volume-title":"Principles and procedures for blood cultures. Approved guideline","author":"Clinical and Laboratory Standards Institute","year":"2009","unstructured":"Clinical and Laboratory Standards Institute. 2009. Principles and procedures for blood cultures. Approved guideline, vol 27, no. 18. CLSI document M47-A. Clinical and Laboratory Standards Institute, Wayne, PA."},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/421087"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1097\/AOG.0b013e3181cbd5f5"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00493567"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis1021"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02064-06"},{"key":"e_1_3_2_9_2","first-page":"235","article-title":"Use of simulated blood cultures for antibiotic effect on time to detection of the two blood culture systems BacT\/Alert and Bactec 9240","volume":"27","author":"Vigan\u00f2 EF","year":"2004","unstructured":"Vigan\u00f2EFVasconiEAgrappiCClericiPMelloniP. 2004. Use of simulated blood cultures for antibiotic effect on time to detection of the two blood culture systems BacT\/Alert and Bactec 9240. New Microbiol. 27:235\u2013248.","journal-title":"New Microbiol."},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2012.03.022"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.3.657-661.1998"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.1.53-58.1997"},{"key":"e_1_3_2_13_2","first-page":"705","volume-title":"Principles and practice of infectious diseases","author":"Amsden GW","year":"2010","unstructured":"AmsdenGW. 2010. Tables of antimicrobial agent pharmacology, p 705\u2013761. In MandellGLBennettJEDolinR (ed), Principles and practice of infectious diseases, 7th ed, vol 1. 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Centers for Disease Control and Prevention, Atlanta, GA. http:\/\/www.cdc.gov\/growthcharts\/clinical_charts.htm."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e318187ad5a"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2007-3423"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e3181c767b6"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1097\/INF.0b013e318271c4e0"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-11-279"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1086\/510386"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1111\/1469-0691.12132"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-135-7-200110020-00007"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02205-13","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02205-13","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:27:55Z","timestamp":1646479675000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02205-13"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,12]]},"references-count":24,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2013,12]]}},"alternative-id":["10.1128\/JCM.02205-13"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02205-13","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,12]]},"assertion":[{"value":"2013-08-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-09-25","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-11-15","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}