{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T09:25:27Z","timestamp":1791192327901,"version":"4.1.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2010,4,1]],"date-time":"2010-04-01T00:00:00Z","timestamp":1270080000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Rapid and sensitive detection of\n            <jats:italic>Aspergillus<\/jats:italic>\n            from clinical samples may facilitate the early diagnosis of invasive pulmonary aspergillosis (IPA). A real-time nucleic acid sequence-based amplification (NASBA) method was investigated by use of an inhalational rat model of IPA. Immunosuppressed male Sprague-Dawley rats were exposed to\n            <jats:italic>Aspergillus fumigatus<\/jats:italic>\n            spores for an hour in an aerosol chamber. Bronchoalveolar lavage (BAL) fluid, lung tissues, and whole blood were collected from five infected rats at 1, 24, 48, 72, and 96 h postinfection and five uninfected rats at the end of the experiment. Total nucleic acid (TNA) was extracted on an easyMAG instrument. A primer-molecular beacon set targeting 28S rRNA was designed to detect\n            <jats:italic>Aspergillus<\/jats:italic>\n            spp. The results were compared to those of quantitative PCR (qPCR) (18S rDNA) and quantitative culture. The analytical sensitivity of the real-time NASBA assay was &lt;1 CFU\/assay. A linear range of detection was demonstrated over 5 log units of conidia (10 to 10\n            <jats:sup>5<\/jats:sup>\n            spores). Both NASBA and qPCR showed a progressive increase in lung tissue burdens, while the CFU counts were stable over time. The fungal burdens in BAL fluid were more variable and not indicative of a progressive infection. The results of both real-time assays correlated well for both sample types (\n            <jats:italic>r<\/jats:italic>\n            = 0.869 and\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.0001 for lung tissue,\n            <jats:italic>r<\/jats:italic>\n            = 0.887 and\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.0001 for BAL fluid). For all whole-blood specimens, NASBA identified\n            <jats:italic>Aspergillus<\/jats:italic>\n            -positive samples in the group from which samples were collected at 72 h postinfection (three of five samples) and the group from which samples were collected at 96 h postinfection (five of five samples), but no positive results were obtained by culture or PCR. Real-time NASBA is highly sensitive and useful for the detection of\n            <jats:italic>Aspergillus<\/jats:italic>\n            in an experimental model of IPA.\n          <\/jats:p>","DOI":"10.1128\/jcm.02214-09","type":"journal-article","created":{"date-parts":[[2010,2,4]],"date-time":"2010-02-04T02:24:21Z","timestamp":1265250261000},"page":"1378-1383","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":39,"title":["Detection of\n            <i>Aspergillus fumigatus<\/i>\n            in a Rat Model of Invasive Pulmonary Aspergillosis by Real-Time Nucleic Acid Sequence-Based Amplification"],"prefix":"10.1128","volume":"48","author":[{"given":"Yanan","family":"Zhao","sequence":"first","affiliation":[{"name":"Public Health Research Institute, UMDNJ-NJMS, Newark, New Jersey 07103"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steven","family":"Park","sequence":"additional","affiliation":[{"name":"Public Health Research Institute, UMDNJ-NJMS, Newark, New Jersey 07103"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter","family":"Warn","sequence":"additional","affiliation":[{"name":"University of Manchester, Manchester, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raghdaa","family":"Shrief","sequence":"additional","affiliation":[{"name":"University of Manchester, Manchester, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elizabeth","family":"Harrison","sequence":"additional","affiliation":[{"name":"University of Manchester, Manchester, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David S.","family":"Perlin","sequence":"additional","affiliation":[{"name":"Public Health Research Institute, UMDNJ-NJMS, Newark, New Jersey 07103"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1086\/323335"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1080\/mmy.40.4.333.357"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijfoodmicro.2007.02.007"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/350091a0"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1086\/513943"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02246-07"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.01635-06"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-7012(99)00061-5"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-003-0989-0"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bmt.1704768"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1086\/323337"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2006.01498.x"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.7.3136-3139.2005"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.4.1626-1629.2001"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1086\/339824"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2006.04.012"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1086\/429921"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02187-05"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.6.1625-1629.1998"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2006.11.011"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.12.5676-5682.2003"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1080\/13693780802126583"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.5.2184-2186.2003"},{"key":"e_1_3_2_25_2","first-page":"129","volume":"52","year":"2009","unstructured":"Ramirez, M., C. Castro, J. C. Palomares, M. J. Torres, A. I. Aller, M. Ruiz, J. Aznar, and E. Martin-Mazuelos. 2009. Molecular detection and identification of Aspergillus spp. from clinical samples using real-time PCR. Mycoses 52 : 129-134.","journal-title":"Mycoses"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1128\/AEM.71.7.3734-3740.2005"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.94.4.745"},{"key":"e_1_3_2_28_2","first-page":"1322","volume":"12","year":"2005","unstructured":"Scotter, J. M., and S. T. Chambers. 2005. Comparison of galactomannan detection, PCR-enzyme-linked immunosorbent assay, and real-time PCR for diagnosis of invasive aspergillosis in a neutropenic rat model and effect of caspofungin acetate. Clin. Diagn. Lab. Immunol. 12 : 1322-1327.","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00787-06"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2005.01349.x"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1472-765x.2000.00670.x"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.5.1811-1818.2003"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(20010115)91:2<311::AID-CNCR1003>3.0.CO;2-3"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1038\/nbt0396-303"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00276-08"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.19.1.95-110.2006"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.3346\/jkms.2007.22.4.672"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02230-08"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02214-09","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02214-09","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T09:32:13Z","timestamp":1646472733000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02214-09"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,4]]},"references-count":37,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2010,4]]}},"alternative-id":["10.1128\/JCM.02214-09"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02214-09","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,4]]},"assertion":[{"value":"2009-11-12","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-01-22","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2010-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}