{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T19:22:08Z","timestamp":1790709728780,"version":"4.1.0"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2021,1,21]],"date-time":"2021-01-21T00:00:00Z","timestamp":1611187200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2021,1,21]],"date-time":"2021-01-21T00:00:00Z","timestamp":1611187200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2021,1,21]]},"abstract":"<jats:p>\n            Recent studies have highlighted the interest in noninvasive sampling procedures coupled with real-time PCR methods for the detection of\n            <jats:italic>Leishmania<\/jats:italic>\n            species in South America. In French Guiana, the sampling method still relied on skin biopsies. Noninvasive protocols should be tested on a large annual cohort to improve routine laboratory diagnosis of cutaneous leishmaniasis. Therefore, we evaluated the performance of a new\n            <jats:italic>Leishmania<\/jats:italic>\n            detection and species identification protocol involving cotton swabs and SYBR green-based real-time PCR of the Hsp70 gene, coupled with Sanger sequencing.\n          <\/jats:p>","DOI":"10.1128\/jcm.02218-20","type":"journal-article","created":{"date-parts":[[2020,10,30]],"date-time":"2020-10-30T14:02:15Z","timestamp":1604066535000},"update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Validation of Swab Sampling and SYBR Green-Based Real-Time PCR for the Diagnosis of Cutaneous Leishmaniasis in French Guiana"],"prefix":"10.1128","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3695-6824","authenticated-orcid":false,"given":"Romain","family":"Blaizot","sequence":"first","affiliation":[{"name":"Department of Dermatology, Andr\u00e9e Rosemon Hospital, Cayenne, French Guiana"},{"name":"Tropical Biome and Immunophysiopathology (TBIP), Universit\u00e9 de Lille, CNRS, INSERM, Institut Pasteur de Lille, U1019-UMR9017-CIIL-Centre d'Infection et d\u2019Immunit\u00e9 de Lille, Centre Hospitalier de Cayenne, Universit\u00e9 de Guyane, Cayenne, French Guiana"},{"name":"National Reference Centre for Cutaneous Leishmaniasis (associate laboratory), Cayenne, French Guiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Simon","sequence":"additional","affiliation":[{"name":"Tropical Biome and Immunophysiopathology (TBIP), Universit\u00e9 de Lille, CNRS, INSERM, Institut Pasteur de Lille, U1019-UMR9017-CIIL-Centre d'Infection et d\u2019Immunit\u00e9 de Lille, Centre Hospitalier de Cayenne, Universit\u00e9 de Guyane, Cayenne, French Guiana"},{"name":"National Reference Centre for Cutaneous Leishmaniasis (associate laboratory), Cayenne, French Guiana"},{"name":"Laboratory of Parasitology-Mycology, Andr\u00e9e Rosemon 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Cayenne, French Guiana"},{"name":"Tropical Biome and Immunophysiopathology (TBIP), Universit\u00e9 de Lille, CNRS, INSERM, Institut Pasteur de Lille, U1019-UMR9017-CIIL-Centre d'Infection et d\u2019Immunit\u00e9 de Lille, Centre Hospitalier de Cayenne, Universit\u00e9 de Guyane, Cayenne, French Guiana"},{"name":"National Reference Centre for Cutaneous Leishmaniasis (associate laboratory), Cayenne, French Guiana"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Magalie","family":"Demar","sequence":"additional","affiliation":[{"name":"Tropical Biome and Immunophysiopathology (TBIP), Universit\u00e9 de Lille, CNRS, INSERM, Institut Pasteur de Lille, U1019-UMR9017-CIIL-Centre d'Infection et d\u2019Immunit\u00e9 de Lille, Centre Hospitalier de Cayenne, Universit\u00e9 de Guyane, Cayenne, French Guiana"},{"name":"National Reference Centre for Cutaneous Leishmaniasis (associate laboratory), Cayenne, French Guiana"},{"name":"Laboratory of Parasitology-Mycology, Andr\u00e9e 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