{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T15:36:28Z","timestamp":1782488188024,"version":"3.54.5"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"12","license":[{"start":{"date-parts":[[2013,12,1]],"date-time":"2013-12-01T00:00:00Z","timestamp":1385856000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,12]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Next-generation DNA sequencing can be used to catalog individual organisms within complex, polymicrobial specimens. Here, we utilized deep sequencing of 16S rRNA to implicate\n            <jats:named-content content-type=\"genus-species\">Actinomadura madurae<\/jats:named-content>\n            as the cause of mycetoma in a diabetic patient when culture and conventional molecular methods were overwhelmed by overgrowth of other organisms.\n          <\/jats:p>","DOI":"10.1128\/jcm.02227-13","type":"journal-article","created":{"date-parts":[[2013,10,10]],"date-time":"2013-10-10T14:56:44Z","timestamp":1381417004000},"page":"4262-4265","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Molecular Diagnosis of Actinomadura madurae Infection by 16S rRNA Deep Sequencing"],"prefix":"10.1128","volume":"51","author":[{"given":"Stephen J.","family":"Salipante","sequence":"first","affiliation":[{"name":"Departments of Laboratory Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dhruba J.","family":"SenGupta","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel R.","family":"Hoogestraat","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa A.","family":"Cummings","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bronwyn H.","family":"Bryant","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Natividad","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephanie","family":"Thielges","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Peter W.","family":"Monsaas","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mimosa","family":"Chau","sequence":"additional","affiliation":[{"name":"Departments of Laboratory Medicine, University of Washington, Seattle, Washington, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lindley A.","family":"Barbee","sequence":"additional","affiliation":[{"name":"Medicine, University of Washington, Seattle, Washington, USA"},{"name":"Sexually Transmitted Diseases Program, Public Health\u2014Seattle &amp; 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