{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:31:14Z","timestamp":1787016674697,"version":"build-2736575974"},"reference-count":18,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["A100420"],"award-info":[{"award-number":["A100420"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The Aptima Combo 2 (AC2) and Aptima CT (ACT) (Hologic Inc., San Diego, CA) are nucleic acid amplification tests (NAATs) that detect\n                    <jats:named-content content-type=\"genus-species\">Chlamydia trachomatis<\/jats:named-content>\n                    . AC2 also detects\n                    <jats:named-content content-type=\"genus-species\">Neisseria gonorrhoeae<\/jats:named-content>\n                    . Storage and temperature conditions may impact the utility of NAATs in some settings and screening programs. We evaluated specimen stability for use beyond the Aptima package insert specifications for temperature and duration of storage (between 2\u00b0C and 30\u00b0C and 60 days, respectively) in two studies: (i) dry\n                    <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n                    -seeded swabs were used with ACT after storage at 4\u00b0C, 23\u00b0C, or 36\u00b0C for up to 84 days and (ii) swabs seeded with\n                    <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n                    and\n                    <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n                    and then placed in transport medium were tested with AC2, after being mailed via the U.S. Postal Service to three different sites. Prolonged storage of samples had no effect, and samples stored at 4\u00b0C, 23\u00b0C, and 36\u00b0C for up to 84 days yielded comparable ACT positivities, although there was a drop in signal intensity for virtually all specimens under all storage\/shipping conditions after day 21. In the mailing study, 80%, 52% and 29% of seeded swabs were exposed to temperatures of &gt;30\u00b0C during three rounds in transit, and 2% reached temperatures of &gt;40\u00b0C. No evidence of signal degradation in the AC2 assay for detection of\n                    <jats:named-content content-type=\"genus-species\">C. trachomatis<\/jats:named-content>\n                    or\n                    <jats:named-content content-type=\"genus-species\">N. gonorrhoeae<\/jats:named-content>\n                    was observed, although some mailed swabs took more than 5 weeks to reach the laboratory site. These two studies support the potential use of swabs at temperatures above 36\u00b0C and storage beyond 60 days and provide confidence regarding this commercially available NAAT for testing of specimens after mailing.\n                  <\/jats:p>","DOI":"10.1128\/jcm.02235-16","type":"journal-article","created":{"date-parts":[[2017,1,11]],"date-time":"2017-01-11T21:41:45Z","timestamp":1484170905000},"page":"971-977","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":20,"title":["Stability Studies on Dry Swabs and Wet Mailed Swabs for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae in Aptima Assays"],"prefix":"10.1128","volume":"55","author":[{"given":"Jeanne","family":"Moncada","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, University of California, San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carey B.","family":"Clark","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, University of Alabama, Birmingham, Alabama, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey","family":"Holden","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Johns Hopkins University, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"III","given":"Edward W.","family":"Hook","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, University of Alabama, Birmingham, Alabama, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte A.","family":"Gaydos","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Johns Hopkins University, Baltimore, Maryland, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julius","family":"Schachter","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, University of California, San Francisco, California, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e31820a94d2"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.1.127-131.2005"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.06481-11"},{"key":"e_1_3_2_5_2","first-page":"1","article-title":"Sexually transmitted diseases treatment guidelines, 2015","author":"Workowski KA","year":"2015","unstructured":"Workowski KA Bolan GA , Centers for Disease Control and Prevention. 2015. Sexually transmitted diseases treatment guidelines, 2015. MMWR Recomm Rep64(RR-03):1\u2013137.","journal-title":"MMWR Recomm Rep"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e31817bdd7e"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.1.304-309.2003"},{"key":"e_1_3_2_8_2","volume-title":"Package insert","author":"Hologic Inc","year":"2012","unstructured":"Hologic Inc. 2012. APTIMA unisex swab specimen collection kit for endocervical and male urethral swab specimens. Package insert. Hologic Inc, San Diego, CA."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(98)12387-5"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03218-14"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.3.758-761.2002"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02387-08"},{"key":"e_1_3_2_13_2","first-page":"1","article-title":"Recommendations for the laboratory-based detection of Chlamydia trachomatis and Neisseria gonorrhoeae\u20142014","author":"Centers for Disease Control and Prevention","year":"2014","unstructured":"Centers for Disease Control and Prevention. 2014. Recommendations for the laboratory-based detection of Chlamydia trachomatis and Neisseria gonorrhoeae\u20142014. MMWR Recomm Rep63(RR-02):1\u201319.","journal-title":"MMWR Recomm Rep"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1097\/OLQ.0b013e31815d968d"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1097\/00007435-200205000-00008"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2012.02.008"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02620-05"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02301-07"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.6.4.328-331.1977"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02235-16","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02235-16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T05:07:12Z","timestamp":1646456832000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02235-16"}},"subtitle":[],"editor":[{"given":"Erik","family":"Munson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,3]]},"references-count":18,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,3]]}},"alternative-id":["10.1128\/JCM.02235-16"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02235-16","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,3]]},"assertion":[{"value":"2016-11-16","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-12-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-02-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}