{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T19:07:32Z","timestamp":1790968052847,"version":"4.1.0"},"reference-count":49,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2008,6,1]],"date-time":"2008-06-01T00:00:00Z","timestamp":1212278400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2008,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Bacterial identification and antibiotic susceptibility testing currently require 48 h when a first blood culture (BC) is positive for clustered gram-positive cocci on direct smear examination (DSE). Meanwhile, antibiotic treatment is often inadequate, reducing the chances of effective treatment or creating unnecessary selective pressure. A new real-time PCR (RT-PCR) technique that differentiates\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            from coagulase-negative staphylococci (CoNS) and detects methicillin resistance in 90 min in BC bottles could help solve these problems. BC bottles from 410 patients with gram-positive cocci on DSE were processed by current methods, and patients' treatments were prospectively recorded. The RT-PCR assay was performed on aliquots of these BCs, which had been kept frozen. For the 121 patients who had true bacteremia, we established whether the faster availability of RT-PCR results could have led to the initiation of treatments different from those actually given. RT-PCR sensitivity and specificity were 100% for differentiating between\n            <jats:italic>S. aureus<\/jats:italic>\n            and CoNS and detecting methicillin resistance with two manufacturers' BC bottles. For 31\/86 (36%) of the\n            <jats:italic>S. aureus<\/jats:italic>\n            -infected patients and for 8\/35 (23%) of the CoNS-infected patients who either had suboptimal or nonoptimal treatment or were untreated 48 h after positivity was detected, the early availability of RT-PCR results could have allowed more effective treatment. Unnecessary glycopeptide treatments could have been avoided for 28 additional patients. The use of RT-PCR would increase treatment effectiveness in patients with staphylococcal bacteremia and reduce the selective pressure created by glycopeptides.\n          <\/jats:p>","DOI":"10.1128\/jcm.02250-07","type":"journal-article","created":{"date-parts":[[2008,4,17]],"date-time":"2008-04-17T01:38:39Z","timestamp":1208396319000},"page":"2045-2051","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Accuracy and Potential Usefulness of Triplex Real-Time PCR for Improving Antibiotic Treatment of Patients with Blood Cultures Showing Clustered Gram-Positive Cocci on Direct Smears"],"prefix":"10.1128","volume":"46","author":[{"given":"Raymond","family":"Ruimy","sequence":"first","affiliation":[{"name":"Hospital Group Bichat-Claude Bernard, AP-HP, and EA 3964, University Denis Diderot-Paris 7 Medical School, 75870 Paris, Cedex 18, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie","family":"Dos-Santos","sequence":"additional","affiliation":[{"name":"Hospital Group Bichat-Claude Bernard, AP-HP, and EA 3964, University Denis Diderot-Paris 7 Medical School, 75870 Paris, Cedex 18, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laurent","family":"Raskine","sequence":"additional","affiliation":[{"name":"Hospital Lariboisie\u0300re, AP-HP, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fre\u0301de\u0301ric","family":"Bert","sequence":"additional","affiliation":[{"name":"Hospital Beaujon, AP-HP, and EA 3964, University Denis Diderot-Paris 7 Medical School, Clichy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rene\u0301","family":"Masson","sequence":"additional","affiliation":[{"name":"Hospital Group Bichat-Claude Bernard, AP-HP, and EA 3964, University Denis Diderot-Paris 7 Medical School, 75870 Paris, Cedex 18, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandrine","family":"Elbaz","sequence":"additional","affiliation":[{"name":"Hospital Group Bichat-Claude Bernard, AP-HP, and EA 3964, University Denis Diderot-Paris 7 Medical School, 75870 Paris, Cedex 18, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Bonnal","sequence":"additional","affiliation":[{"name":"Hospital Group Bichat-Claude Bernard, AP-HP, and EA 3964, University Denis Diderot-Paris 7 Medical School, 75870 Paris, Cedex 18, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean-Christophe","family":"Lucet","sequence":"additional","affiliation":[{"name":"Hospital Group Bichat-Claude Bernard, AP-HP, and EA 3964, University Denis Diderot-Paris 7 Medical School, 75870 Paris, Cedex 18, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agne\u0300s","family":"Lefort","sequence":"additional","affiliation":[{"name":"Hospital Beaujon, AP-HP, and EA 3964, University Denis Diderot-Paris 7 Medical School, Clichy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruno","family":"Fantin","sequence":"additional","affiliation":[{"name":"Hospital Beaujon, AP-HP, and EA 3964, University Denis Diderot-Paris 7 Medical School, Clichy, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michel","family":"Wolff","sequence":"additional","affiliation":[{"name":"Hospital Group Bichat-Claude Bernard, AP-HP, and EA 3964, University Denis Diderot-Paris 7 Medical School, 75870 Paris, Cedex 18, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michele","family":"Hornstein","sequence":"additional","affiliation":[{"name":"Hospital Avicenne, AP-HP, Bobigny, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antoine","family":"Andremont","sequence":"additional","affiliation":[{"name":"Hospital Group Bichat-Claude Bernard, AP-HP, and EA 3964, University Denis Diderot-Paris 7 Medical School, 75870 Paris, Cedex 18, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.6.2932-2933.2005"},{"key":"e_1_3_2_3_2","unstructured":"Communiqu\u00e9 du Comit\u00e9 de l'Antibiogramme de la Soci\u00e9t\u00e9 Fran\u00e7aise de Microbiologie. 2006"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1086\/502584"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/48.suppl_1.65"},{"key":"e_1_3_2_6_2","first-page":"565","volume":"51","year":"2002","unstructured":"Centers for Disease Control and Prevention. 2002. Staphylococcus aureus resistant to vancomycin\u2014United States, 2002. MMWR Morb. Mortal. Wkly. Rep.51:565-567.","journal-title":"MMWR Morb. Mortal. Wkly. Rep."},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.md.0000091184.93122.09"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.9.4324-4327.2003"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.3.1283-1284.2004"},{"key":"e_1_3_2_10_2","unstructured":"EARSS manual. 2004"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/20.1.16"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.1.254-260.2003"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.7.2392-2397.2002"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.8.3942-3944.2003"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1001\/archinte.158.2.182"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.5.1875-1884.2004"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.118.1.146"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0163-4453(97)92395-1"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1080\/00365540210147994"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.7.2516-2519.2000"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268800051682"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/clind\/16.4.567"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1086\/375057"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.8.2786-2790.2002"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.4.1514-1517.2002"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01690549"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.128.4.2732"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.6.1363"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.2.889-891.2003"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0732-8893(02)00542-4"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1309\/6EA3U9V8NCLLGKQN"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.1994.03510440058033"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.2307\/30146486"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajic.2005.03.004"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/22.2.276"},{"key":"e_1_3_2_36_2","first-page":"275","volume":"70","year":"2004","unstructured":"Shlaes, D. M., S. J. Projan, and J. E. Edwards, Jr. 2004. Antibiotic discovery: state of the state. ASM News70:275-281.","journal-title":"ASM News"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.7.2659-2661.2002"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.4.1109-1112.1998"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.4.1032-1034.1998"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00552-07"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1086\/510386"},{"key":"e_1_3_2_42_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.12.4529-4531.2001"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2006.09.003"},{"key":"e_1_3_2_45_2","first-page":"8","year":"2005","unstructured":"Turnidge, J. D., and J. M. Bell. 2005. Antimicrobial susceptibility on solid media, p. 8-60. In V. Lorian (ed.), Antibiotics in laboratory medicine. Lippincott Williams and Willkins, Baltimore, MD.","journal-title":"Antibiotics in laboratory medicine."},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.30.7.1685-1691.1992"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijantimicag.2003.07.003"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1378\/chest.123.5.1615"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(02)00438-3"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.38.5.1023"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02250-07","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02250-07","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T06:24:32Z","timestamp":1646461472000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02250-07"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,6]]},"references-count":49,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2008,6]]}},"alternative-id":["10.1128\/JCM.02250-07"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02250-07","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,6]]},"assertion":[{"value":"2007-11-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-04-09","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-06-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}