{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:05:31Z","timestamp":1791299131873,"version":"4.1.0"},"reference-count":10,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2011,6,1]],"date-time":"2011-06-01T00:00:00Z","timestamp":1306886400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            A multicenter evaluation was undertaken to evaluate the performance of a new three-color peptide nucleic acid fluorescence\n            <jats:named-content content-type=\"genus-species\">in situ<\/jats:named-content>\n            hybridization assay that identifies isolates directly from blood cultures positive for Gram-negative bacilli (GNB). The assay correctly identified 100% (186\/186) of the\n            <jats:named-content content-type=\"genus-species\">Escherichia coli<\/jats:named-content>\n            isolates, 99.1% (109\/110) of the\n            <jats:named-content content-type=\"genus-species\">Klebsiella pneumoniae<\/jats:named-content>\n            isolates, and 95.8% (46\/48) of the\n            <jats:named-content content-type=\"genus-species\">Pseudomonas aeruginosa<\/jats:named-content>\n            isolates in this study. Negative assay results were correctly obtained for 162 of 165 other GNB (specificity, 98.2%).\n          <\/jats:p>","DOI":"10.1128\/jcm.02250-10","type":"journal-article","created":{"date-parts":[[2011,4,14]],"date-time":"2011-04-14T02:45:55Z","timestamp":1302749155000},"page":"2259-2261","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":23,"title":["Identification of Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa in Blood Cultures: a Multicenter Performance Evaluation of a Three-Color Peptide Nucleic Acid Fluorescence In Situ Hybridization Assay"],"prefix":"10.1128","volume":"49","author":[{"given":"Phyllis","family":"Della-Latta","sequence":"first","affiliation":[{"name":"Columbia University Medical Center, NewYork-Presbyterian Hospital, New York, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hossein","family":"Salimnia","sequence":"additional","affiliation":[{"name":"Detroit Medical Center and Wayne State University School of Medicine, Detroit, Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Theresa","family":"Painter","sequence":"additional","affiliation":[{"name":"Detroit Medical Center, Detroit, Michigan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fann","family":"Wu","sequence":"additional","affiliation":[{"name":"Columbia University Medical Center, NewYork-Presbyterian Hospital, New York, New York"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gary W.","family":"Procop","sequence":"additional","affiliation":[{"name":"Cleveland Clinic, Cleveland, Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deborah A.","family":"Wilson","sequence":"additional","affiliation":[{"name":"Cleveland Clinic, Cleveland, Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wendy","family":"Gillespie","sequence":"additional","affiliation":[{"name":"Lahey Clinic, Burlington, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alayna","family":"Mender","sequence":"additional","affiliation":[{"name":"AdvanDx Inc., Woburn, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin S.","family":"Crystal","sequence":"additional","affiliation":[{"name":"AdvanDx Inc., Woburn, Massachusetts"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2005.10.011"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00751-06"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkl146"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/432803"},{"key":"e_1_3_2_6_2","volume-title":"Abstr. 20th ESCMID","author":"Hansen D. 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