{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T04:57:04Z","timestamp":1790485024191,"version":"4.1.0"},"reference-count":52,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2016,1,1]],"date-time":"2016-01-01T00:00:00Z","timestamp":1451606400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"CDC","award":["200-2013-M-57203"],"award-info":[{"award-number":["200-2013-M-57203"]}]},{"name":"NIH","award":["K24AI102972"],"award-info":[{"award-number":["K24AI102972"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2016,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    Acute febrile illness (AFI) is associated with substantial morbidity and mortality worldwide, yet an etiologic agent is often not identified. Convalescent-phase serology is impractical, blood culture is slow, and many pathogens are fastidious or impossible to cultivate. We developed a real-time PCR-based TaqMan array card (TAC) that can test six to eight samples within 2.5 h from sample to results and can simultaneously detect 26 AFI-associated organisms, including 15 viruses (chikungunya, Crimean-Congo hemorrhagic fever [CCHF] virus, dengue, Ebola virus, Bundibugyo virus, Sudan virus, hantaviruses [Hantaan and Seoul], hepatitis E, Marburg, Nipah virus, o'nyong-nyong virus, Rift Valley fever virus, West Nile virus, and yellow fever virus), 8 bacteria (\n                    <jats:named-content content-type=\"genus-species\">Bartonella<\/jats:named-content>\n                    spp.,\n                    <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n                    spp.,\n                    <jats:named-content content-type=\"genus-species\">Coxiella burnetii<\/jats:named-content>\n                    ,\n                    <jats:named-content content-type=\"genus-species\">Leptospira<\/jats:named-content>\n                    spp.,\n                    <jats:named-content content-type=\"genus-species\">Rickettsia<\/jats:named-content>\n                    spp.,\n                    <jats:named-content content-type=\"genus-species\">Salmonella enterica<\/jats:named-content>\n                    and\n                    <jats:named-content content-type=\"genus-species\">Salmonella enterica<\/jats:named-content>\n                    serovar Typhi, and\n                    <jats:named-content content-type=\"genus-species\">Yersinia pestis<\/jats:named-content>\n                    ), and 3 protozoa (\n                    <jats:named-content content-type=\"genus-species\">Leishmania<\/jats:named-content>\n                    spp.,\n                    <jats:named-content content-type=\"genus-species\">Plasmodium<\/jats:named-content>\n                    spp., and\n                    <jats:named-content content-type=\"genus-species\">Trypanosoma brucei<\/jats:named-content>\n                    ). Two extrinsic controls (phocine herpesvirus 1 and bacteriophage MS2) were included to ensure extraction and amplification efficiency. Analytical validation was performed on spiked specimens for linearity, intra-assay precision, interassay precision, limit of detection, and specificity. The performance of the card on clinical specimens was evaluated with 1,050 blood samples by comparison to the individual real-time PCR assays, and the TAC exhibited an overall 88% (278\/315; 95% confidence interval [CI], 84% to 92%) sensitivity and a 99% (5,261\/5,326, 98% to 99%) specificity. This TaqMan array card can be used in field settings as a rapid screen for outbreak investigation or for the surveillance of pathogens, including Ebola virus.\n                  <\/jats:p>","DOI":"10.1128\/jcm.02257-15","type":"journal-article","created":{"date-parts":[[2015,10,22]],"date-time":"2015-10-22T11:02:03Z","timestamp":1445511723000},"page":"49-58","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":126,"title":["Development of a TaqMan Array Card for Acute-Febrile-Illness Outbreak Investigation and Surveillance of Emerging Pathogens, Including Ebola Virus"],"prefix":"10.1128","volume":"54","author":[{"given":"Jie","family":"Liu","sequence":"first","affiliation":[{"name":"Division of Infectious Diseases and International Health, University of Virginia, Charlottesville, Virginia, 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