{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T04:59:33Z","timestamp":1782190773531,"version":"3.54.5"},"reference-count":20,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2008,5,1]],"date-time":"2008-05-01T00:00:00Z","timestamp":1209600000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2008,5]]},"abstract":"ABSTRACT<\/jats:title>\n \n The present study was conducted to compare two stool antigen detection kits with PCR for the diagnosis of\n Entamoeba histolytica<\/jats:italic>\n infections by using fecal specimens submitted to the Department of Microbiology at St. Vincent's Hospital, Sydney, and the Institute of Medical and Veterinary Science, Adelaide, Australia. A total of 279 stool samples containing the E complex (\n E. histolytica<\/jats:italic>\n ,\n Entamoeba dispar<\/jats:italic>\n , and\n Entamoeba moshkovskii<\/jats:italic>\n ) were included in this study. The stool specimens were tested by using two commercially produced enzyme immunoassays (the Entamoeba CELISA PATH and TechLab\n E. histolytica<\/jats:italic>\n II kits) to detect antigens of\n E. histolytica<\/jats:italic>\n . DNA was extracted from all of the samples with a Qiagen DNA stool mini kit (Qiagen, Hilden, Germany), and a PCR targeting the small-subunit ribosomal DNA was performed on all of the samples. When PCR was used as a reference standard, the CELISA PATH kit showed 28% sensitivity and 100% specificity. The TechLab ELISA (enzyme-linked immunosorbent assay) kit did not prove to be useful in detecting\n E. histolytica<\/jats:italic>\n , as it failed to identify any of the\n E. histolytica<\/jats:italic>\n samples which were positive by PCR. With the TechLab kit, cross-reactivity was observed for three specimens, one of which was positive for both\n E. dispar<\/jats:italic>\n and\n E. moshkovskii<\/jats:italic>\n while the other two samples contained\n E. moshkovskii<\/jats:italic>\n . Quantitative assessment of the PCR and ELISA results obtained showed that the ELISA kits were 1,000 to 10,000 times less sensitive, and our results show that the CELISA PATH kit and the TechLab ELISA are not useful for the detection of\n E. histolytica<\/jats:italic>\n in stool samples from patients in geographical regions where this parasite is not endemic.\n <\/jats:p>","DOI":"10.1128\/jcm.02261-07","type":"journal-article","created":{"date-parts":[[2008,3,26]],"date-time":"2008-03-26T21:00:42Z","timestamp":1206565242000},"page":"1678-1681","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":65,"title":["Comparison of Stool Antigen Detection Kits to PCR for Diagnosis of Amebiasis"],"prefix":"10.1128","volume":"46","author":[{"given":"D.","family":"Stark","sequence":"first","affiliation":[{"name":"St. Vincent's Hospital, Department of Microbiology, Sydney, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"van Hal","sequence":"additional","affiliation":[{"name":"St. Vincent's Hospital, Department of Microbiology, Sydney, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"R.","family":"Fotedar","sequence":"additional","affiliation":[{"name":"St. Vincent's Hospital, Department of Microbiology, Sydney, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Butcher","sequence":"additional","affiliation":[{"name":"Institute of Medical and Veterinary Science, The Queen Elizabeth Hospital, Woodville, South Australia, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Marriott","sequence":"additional","affiliation":[{"name":"St. Vincent's Hospital, Department of Microbiology, Sydney, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Ellis","sequence":"additional","affiliation":[{"name":"University of Technology Sydney, Department of Medical and Molecular Biosciences, Broadway, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Harkness","sequence":"additional","affiliation":[{"name":"St. Vincent's Hospital, Department of Microbiology, Sydney, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid0905.020548"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.15.3.329-341.2002"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1550-7408.1993.tb04926.x"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02144-06"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00004-07"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2002.67.123"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.1.237-241.2003"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.47.3.236"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/175.3.734"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.2.449-452.1998"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.10.2558-2561.1995"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.9.2405-2407.1997"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.5.2168-2172.2005"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00530-06"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2007.76.549"},{"key":"e_1_3_1_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.16.4.713-729.2003"},{"key":"e_1_3_1_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.35.7.1701-1705.1997"},{"key":"e_1_3_1_19_2","doi-asserted-by":"publisher","DOI":"10.5694\/j.1326-5377.2007.tb00975.x"},{"key":"e_1_3_1_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijmm.2006.03.001"},{"key":"e_1_3_1_21_2","first-page":"97","volume":"72","year":"1997","unstructured":"World Health Organization. 1997. 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Rec."}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02261-07","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02261-07","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T01:22:42Z","timestamp":1646443362000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02261-07"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2008,5]]},"references-count":20,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2008,5]]}},"alternative-id":["10.1128\/JCM.02261-07"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02261-07","relation":{"has-review":[{"id-type":"doi","id":"10.3410\/f.1108581.564659","asserted-by":"object"}]},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2008,5]]},"assertion":[{"value":"2007-11-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-03-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2008-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}