{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,30]],"date-time":"2026-10-30T22:13:33Z","timestamp":1793398413620,"version":"4.5.0"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2010,8,1]],"date-time":"2010-08-01T00:00:00Z","timestamp":1280620800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>In a previous paper, we reported on a large number of cheetah blood specimens that gave positive signals only for<jats:italic>Babesia<\/jats:italic>and\/or<jats:italic>Theileria<\/jats:italic>genus-specific probes on the reverse line blot (RLB) assay, indicating the presence of a novel species or variant of an existing species. Some of these specimens were investigated further by microscopic, serological, sequencing, and phylogenetic analyses. The near-full-length 18S rRNA genes of 13 samples, as well as the second internal transcribed spacer (ITS2) region, were amplified, cloned, and sequenced. A species-specific RLB probe, designed to target the hypervariable V4 region of the 18S rRNA gene for detection of the novel<jats:italic>Babesia<\/jats:italic>sp., was used to screen an additional 137 cheetah blood specimens for the presence of the species. The prevalence of infection was 28.5%. Here we describe the morphology and phylogenetic relationships of the novel species, which we have named<jats:italic>Babesia lengau<\/jats:italic>sp. nov.<\/jats:p>","DOI":"10.1128\/jcm.02266-09","type":"journal-article","created":{"date-parts":[[2010,6,3]],"date-time":"2010-06-03T02:51:58Z","timestamp":1275533518000},"page":"2703-2708","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":52,"title":["<i>Babesia lengau<\/i>sp. nov., a Novel<i>Babesia<\/i>Species in Cheetah (<i>Acinonyx jubatus<\/i>, Schreber, 1775) Populations in South Africa"],"prefix":"10.1128","volume":"48","author":[{"given":"Anna-Mari","family":"Bosman","sequence":"first","affiliation":[{"name":"Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, 0110 Onderstepoort, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marinda C.","family":"Oosthuizen","sequence":"additional","affiliation":[{"name":"Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, 0110 Onderstepoort, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Peirce","sequence":"additional","affiliation":[{"name":"MP International Consultancy, 6 Normandale House, Normandale, Bexhill-on-Sea, East Sussex TN39 3NZ, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estelle H.","family":"Venter","sequence":"additional","affiliation":[{"name":"Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, 0110 Onderstepoort, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barend L.","family":"Penzhorn","sequence":"additional","affiliation":[{"name":"Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, 0110 Onderstepoort, South Africa"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"Living free. 1962"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.7589\/0090-3558-26.3.392"},{"key":"e_1_3_2_4_2","first-page":"159","volume":"24","year":"1968","unstructured":"Barnett, S. 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