{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,22]],"date-time":"2025-07-22T11:18:41Z","timestamp":1753183121843},"reference-count":29,"publisher":"American Society for Microbiology","issue":"8","license":[{"start":{"date-parts":[[2007,8,1]],"date-time":"2007-08-01T00:00:00Z","timestamp":1185926400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2007,8]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>A uracil-to-cytosine mutation at nucleotide position 472 of oral poliovirus vaccine type 3 (OPV3) contributes to the development of vaccine-associated paralytic poliomyelitis (VAPP). To analyze OPV3 shedding patterns, we previously used the multistep method of mutant analysis by PCR and enzyme cleavage (MAPREC). This involves conventional reverse transcription-PCR to detect OPV3, followed by a restriction digest to quantify position 472 reversion. Real-time PCR detects and quantifies nucleic acid as PCR occurs and avoids postreaction processing. The goal of this study was to compare a real-time PCR method to MAPREC. Seventy-three stool samples from Mexican OPV recipients underwent the reverse transcription-PCR step of MAPREC and real-time PCR. Real-time PCR identified 23% more OPV3-positive samples than conventional reverse transcription-PCR. When reversion was compared, the revertant proportion (RP), defined as the percentage of revertants in a sample, differed by \u226410% in 21\/25 (84%) samples. The four samples differing by &gt;10% were obtained within 5 days of OPV administration. The real-time PCR assay identified samples with an RP of \u226585% with 94% sensitivity and 86% specificity compared to MAPREC. The mean difference in RP between the two methods was 3.6% (95% confidence interval, \u22120.3 to 7.5%). Real-time PCR methods reliably detect OPV3, and reversion estimates correlate more consistently with MAPREC when OPV3 reversion rates are high. Detecting VAPP-related mutations by real-time PCR is rapid and efficient and can be useful in monitoring ongoing global polio eradication efforts.<\/jats:p>","DOI":"10.1128\/jcm.02268-06","type":"journal-article","created":{"date-parts":[[2007,6,21]],"date-time":"2007-06-21T01:44:31Z","timestamp":1182390271000},"page":"2419-2425","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Shedding and Reversion of Oral Polio Vaccine Type 3 in Mexican Vaccinees: Comparison of Mutant Analysis by PCR and Enzyme Cleavage to a Real-Time PCR Assay"],"prefix":"10.1128","volume":"45","author":[{"given":"Devasena","family":"Gnanashanmugam","sequence":"first","affiliation":[{"name":"Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Meira S.","family":"Falkovitz-Halpern","sequence":"additional","affiliation":[{"name":"Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anthony","family":"Dodge","sequence":"additional","affiliation":[{"name":"Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melanie","family":"Fang","sequence":"additional","affiliation":[{"name":"Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lisa J.","family":"Wong","sequence":"additional","affiliation":[{"name":"Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Melissa","family":"Esparza","sequence":"additional","affiliation":[{"name":"Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rebecca","family":"Hammon","sequence":"additional","affiliation":[{"name":"Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Enrique E.","family":"Rivas-Merelles","sequence":"additional","affiliation":[{"name":"Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jose I.","family":"Santos","sequence":"additional","affiliation":[{"name":"Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yvonne","family":"Maldonado","sequence":"additional","affiliation":[{"name":"Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"33","volume":"73","year":"1995","unstructured":"Andrus, J. 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