{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,6]],"date-time":"2026-02-06T03:58:38Z","timestamp":1770350318863,"version":"3.49.0"},"reference-count":66,"publisher":"American Society for Microbiology","issue":"2","license":[{"start":{"date-parts":[[2015,2,1]],"date-time":"2015-02-01T00:00:00Z","timestamp":1422748800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2015,2]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Human papillomavirus (HPV) is causally associated with anal cancer, as HPV DNA is detected in up to 90% of anal intraepithelial neoplasias and anal cancers. With the gradual increase of anal cancer rates, there is a growing need to establish reliable and clinically relevant methods to detect anal cancer precursors. In resource-limited settings, HPV DNA detection is a potentially relevant tool for anal cancer screening. Here, we evaluated the performance of the Hybribio GenoArray (GA) for genotyping HPV in anal samples, against the reference standard Roche Linear Array (LA). Anal swab samples were obtained from sexually active men who have sex with men. Following DNA extraction, each sample was genotyped using GA and LA. The overall interassay agreement, type-specific, and single and multiple genotype agreements were evaluated by kappa statistics and McNemar's \u03c7\n            <jats:sup>2<\/jats:sup>\n            tests. Using GA and LA, 68% and 76% of samples were HPV DNA positive, respectively. There was substantial interassay agreements for the detection of all HPV genotypes (\u03ba = 0.70, 86% agreement). Although LA was able to detect more genotypes per sample, the interassay agreement was acceptable (\u03ba = 0.53, 63% agreement). GA had poorer specific detection of HPV genotypes 35, 42, and 51 (\u03ba &lt; 0.60). In conclusion, GA and LA showed good interassay agreement for the detection of most HPV genotypes in anal samples. However, the detection of HPV DNA in up to 76% of anal samples warrants further evaluation of its clinical significance.\n          <\/jats:p>","DOI":"10.1128\/jcm.02274-14","type":"journal-article","created":{"date-parts":[[2014,12,11]],"date-time":"2014-12-11T02:35:28Z","timestamp":1418265328000},"page":"550-556","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":17,"title":["Comparison of Hybribio GenoArray and Roche Human Papillomavirus (HPV) Linear Array for HPV Genotyping in Anal Swab Samples"],"prefix":"10.1128","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1341-4467","authenticated-orcid":false,"given":"Huey Chi","family":"Low","sequence":"first","affiliation":[{"name":"Centre of Excellence for Research in AIDS, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michelle I.","family":"Silver","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brandon J.","family":"Brown","sequence":"additional","affiliation":[{"name":"Department of Population Health and Disease Prevention, University of California Irvine, Irvine, California, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chan Yoon","family":"Leng","sequence":"additional","affiliation":[{"name":"Centre of Excellence for Research in AIDS, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Magaly M.","family":"Blas","sequence":"additional","affiliation":[{"name":"Epidemiology, STD and HIV Unit, School of Public Health and Administration, Universidad Peruana Cayetano Heredia, Lima, Peru"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patti E.","family":"Gravitt","sequence":"additional","affiliation":[{"name":"Department of Epidemiology, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland, USA"},{"name":"Perdana University Graduate School of Medicine, Serdang, Selangor, Malaysia"},{"name":"Department of Pathology, University of New Mexico Health Sciences Center, Albuquerque, New Mexico, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yin Ling","family":"Woo","sequence":"additional","affiliation":[{"name":"Centre of Excellence for Research in AIDS, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia"},{"name":"Department of Obstetrics and Gynecology, University of Malaya, Kuala Lumpur, Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(04)01410-X"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.20365"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199711063371904"},{"key":"e_1_3_2_5_2","first-page":"1934","article-title":"Human papillomavirus 16 and 18 L1 serology compared across anogenital cancer sites","volume":"61","author":"Carter JJ","year":"2001","unstructured":"Carter JJ, Madeleine MM, Shera K, Schwartz SM, Cushing-Haugen KL, Wipf GC, Porter P, Daling JR, McDougall JK, Galloway DA. 2001. 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FDA: Gardasil approved to prevent anal cancer. 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