{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T22:15:23Z","timestamp":1792880123578,"version":"4.5.0"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T00:00:00Z","timestamp":1301616000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Bacteriological diagnosis of brucellosis is performed by culturing animal samples directly on both Farrell medium (FM) and modified Thayer-Martin medium (mTM). However, despite inhibiting most contaminating microorganisms, FM also inhibits the growth of\n            <jats:named-content content-type=\"genus-species\">Brucella ovis<\/jats:named-content>\n            and some\n            <jats:named-content content-type=\"genus-species\">B. melitensis<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">B. abortus<\/jats:named-content>\n            strains. In contrast, mTM is adequate for growth of all\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            species but only partially inhibitory for contaminants. Moreover, the performance of both culture media for isolating\n            <jats:named-content content-type=\"genus-species\">B. suis<\/jats:named-content>\n            has never been established properly. We first determined the performance of both media for\n            <jats:named-content content-type=\"genus-species\">B. suis<\/jats:named-content>\n            isolation, proving that FM significantly inhibits\n            <jats:named-content content-type=\"genus-species\">B. suis<\/jats:named-content>\n            growth. We also determined the susceptibility of\n            <jats:named-content content-type=\"genus-species\">B. suis<\/jats:named-content>\n            to the antibiotics contained in both selective media, proving that nalidixic acid and bacitracin are highly inhibitory, thus explaining the reduced performance of FM for\n            <jats:named-content content-type=\"genus-species\">B. suis<\/jats:named-content>\n            isolation. Based on these results, a new selective medium (CITA) containing vancomycin, colistin, nystatin, nitrofurantoin, and amphotericin B was tested for isolation of the main\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            species, including\n            <jats:named-content content-type=\"genus-species\">B. suis<\/jats:named-content>\n            . CITA's performance was evaluated using reference contaminant strains but also field samples taken from brucella-infected animals or animals suspected of infection. CITA inhibited most contaminant microorganisms but allowed the growth of all\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            species, to levels similar to those for both the control medium without antibiotics and mTM. Moreover, CITA medium was more sensitive than both mTM and FM for isolating all\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            species from field samples. Altogether, these results demonstrate the adequate performance of CITA medium for the primary isolation of the main\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            species, including\n            <jats:named-content content-type=\"genus-species\">B. suis<\/jats:named-content>\n            .\n          <\/jats:p>","DOI":"10.1128\/jcm.02301-10","type":"journal-article","created":{"date-parts":[[2011,1,27]],"date-time":"2011-01-27T02:44:01Z","timestamp":1296096241000},"page":"1458-1463","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":84,"title":["Development of a Selective Culture Medium for Primary Isolation of the Main Brucella Species"],"prefix":"10.1128","volume":"49","author":[{"given":"M. J.","family":"De Miguel","sequence":"first","affiliation":[{"name":"Unidad de Sanidad Animal, Centro de Investigaci\u00f3n y Tecnolog\u00eda Agroalimentaria (CITA), Gobierno de Arag\u00f3n, Carretera de Monta\u00f1ana 930, 50059 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. M.","family":"Mar\u00edn","sequence":"additional","affiliation":[{"name":"Unidad de Sanidad Animal, Centro de Investigaci\u00f3n y Tecnolog\u00eda Agroalimentaria (CITA), Gobierno de Arag\u00f3n, Carretera de Monta\u00f1ana 930, 50059 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P. M.","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Unidad de Sanidad Animal, Centro de Investigaci\u00f3n y Tecnolog\u00eda Agroalimentaria (CITA), Gobierno de Arag\u00f3n, Carretera de Monta\u00f1ana 930, 50059 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Dieste","sequence":"additional","affiliation":[{"name":"Unidad de Sanidad Animal, Centro de Investigaci\u00f3n y Tecnolog\u00eda Agroalimentaria (CITA), Gobierno de Arag\u00f3n, Carretera de Monta\u00f1ana 930, 50059 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. J.","family":"Grill\u00f3","sequence":"additional","affiliation":[{"name":"Instituto de Agrobiotecnolog\u00eda, CSIC-UPNA-Gobierno de Navarra, Carretera de Mutilva, s\/n 31192, Mutilva Baja, Navarra, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. M.","family":"Blasco","sequence":"additional","affiliation":[{"name":"Unidad de Sanidad Animal, Centro de Investigaci\u00f3n y Tecnolog\u00eda Agroalimentaria (CITA), Gobierno de Arag\u00f3n, Carretera de Monta\u00f1ana 930, 50059 Zaragoza, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","unstructured":"AltonG. G.\nJonesL. M.\nAngusR. D.\nVergerJ. M.. 1988. Techniques for the brucellosis laboratory. INRA Paris France."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2009.01.025"},{"key":"e_1_3_2_4_2","first-page":"265","article-title":"Selective media for the isolation of Brucella ovis","volume":"61","author":"Brown G. M.","year":"1971","unstructured":"BrownG. M. RangerC. R. KelleyD. J.. 1971. Selective media for the isolation of Brucella ovis. 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