{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:04:29Z","timestamp":1790665469313,"version":"4.1.0"},"reference-count":25,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2017,5,23]],"date-time":"2017-05-23T00:00:00Z","timestamp":1495497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2017,6,1]],"date-time":"2017-06-01T00:00:00Z","timestamp":1496275200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"Speedx","award":["2016 Speedx"],"award-info":[{"award-number":["2016 Speedx"]}]}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    <jats:named-content content-type=\"genus-species\">Mycoplasma genitalium<\/jats:named-content>\n                    is a significant pathogen for which first-line treatment is becoming less effective due to increased resistance to macrolides. As conventional culture and antimicrobial susceptibility testing is not feasible for routine detection of this pathogen, molecular markers such as detection of mutations in the 23S rRNA gene have been described to predict resistance. Recently, a novel multiplex quantitative PCR (qPCR) assay, ResistancePlus MG, has been described for the simultaneous detection of\n                    <jats:named-content content-type=\"genus-species\">Mycoplasma genitalium<\/jats:named-content>\n                    and macrolide resistance. In the current study, the clinical performance of the assay was evaluated on 1,089 consecutive urine and anogenital swab samples in symptomatic and asymptomatic male and female patients. Overall, 6.0% were positive for\n                    <jats:named-content content-type=\"genus-species\">M. genitalium<\/jats:named-content>\n                    , with 63.1% having macrolide resistance-associated mutations. Compared to the laboratory-validated qPCR method targeting the 16S rRNA gene and Sanger sequencing to determine 23S rRNA mutations, the sensitivity and specificity of\n                    <jats:named-content content-type=\"genus-species\">M. genitalium<\/jats:named-content>\n                    detection were 98.5% and 100% and for detection of macrolide resistance mutations were 100.0% and 96.2%, respectively. This assay offers a considerable advantage in clinical settings for\n                    <jats:named-content content-type=\"genus-species\">M. genitalium<\/jats:named-content>\n                    testing by making the results of macrolide resistance and mutation analyses simultaneously available, which is increasingly important with escalating macrolide resistance.\n                  <\/jats:p>","DOI":"10.1128\/jcm.02312-16","type":"journal-article","created":{"date-parts":[[2017,4,5]],"date-time":"2017-04-05T20:50:24Z","timestamp":1491425424000},"page":"1915-1919","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":75,"title":["Prospective Evaluation of ResistancePlus MG, a New Multiplex Quantitative PCR Assay for Detection of Mycoplasma genitalium and Macrolide Resistance"],"prefix":"10.1128","volume":"55","author":[{"given":"S. N.","family":"Tabrizi","sequence":"first","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Parkville, Victoria, Australia"},{"name":"Department of Obstetrics and Gynaecology, University of Melbourne, Victoria, Australia"},{"name":"Murdoch Childrens Research Institute, Parkville, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Parkville, Victoria, Australia"},{"name":"Department of Obstetrics and Gynaecology, University of Melbourne, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. S.","family":"Bradshaw","sequence":"additional","affiliation":[{"name":"Melbourne Sexual Health Centre, Carlton, Victoria, Australia"},{"name":"Central Clinical School, Monash University, Frankston, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C. K.","family":"Fairley","sequence":"additional","affiliation":[{"name":"Melbourne Sexual Health Centre, Carlton, Victoria, Australia"},{"name":"Central Clinical School, Monash University, Frankston, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Walker","sequence":"additional","affiliation":[{"name":"SpeeDx Pty Ltd., Sydney, NSW, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L. 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M.","family":"Garland","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Infectious Diseases, The Royal Women's Hospital, Parkville, Victoria, Australia"},{"name":"Department of Obstetrics and Gynaecology, University of Melbourne, Victoria, Australia"},{"name":"Murdoch Childrens Research Institute, Parkville, Victoria, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1136\/sti.2010.044487"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(81)92461-2"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)07848-0"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)65268-8"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2004.00923.x"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1258\/0956462021924776"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1258\/0956462021924695"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1258\/ijsa.2009.009143"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.15585\/mmwr.rr6404a1"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciu1162"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1207.051558"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/cis1022"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0003618"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1086\/593188"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.01233-16"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035593"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00012-16"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/jdv.12963"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.03318-13"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0170087"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0156740"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02328-10"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.4.1451-1455.2002"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1093\/cid\/ciw719"},{"key":"e_1_3_2_26_2","article-title":"Increasing macrolide and fluoroquinolone resistance in Mycoplasma genitalium","author":"Murray GL","unstructured":"Murray GL Bradshaw CS Bissessor M Danielewski J Garland SM Jensen JS Fairley CK Tabrizi SN . Increasing macrolide and fluoroquinolone resistance in Mycoplasma genitalium. 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