{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,27]],"date-time":"2026-02-27T03:29:29Z","timestamp":1772162969260,"version":"3.50.1"},"reference-count":27,"publisher":"American Society for Microbiology","issue":"6","license":[{"start":{"date-parts":[[2010,6,1]],"date-time":"2010-06-01T00:00:00Z","timestamp":1275350400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:p>\n                    The present study was performed to assess the interlaboratory reproducibility of the molecular detection and identification of species of\n                    <jats:italic>Zygomycetes<\/jats:italic>\n                    from formalin-fixed paraffin-embedded kidney and brain tissues obtained from experimentally infected mice. Animals were infected with one of five species (\n                    <jats:italic>Rhizopus oryzae<\/jats:italic>\n                    ,\n                    <jats:italic>Rhizopus microsporus<\/jats:italic>\n                    ,\n                    <jats:italic>Lichtheimia corymbifera<\/jats:italic>\n                    ,\n                    <jats:italic>Rhizomucor pusillus<\/jats:italic>\n                    , and\n                    <jats:italic>Mucor circinelloides<\/jats:italic>\n                    ). Samples with 1, 10, or 30 slide cuts of the tissues were prepared from each paraffin block, the sample identities were blinded for analysis, and the samples were mailed to each of seven laboratories for the assessment of sensitivity. A protocol describing the extraction method and the PCR amplification procedure was provided. The internal transcribed spacer 1 (ITS1) region was amplified by PCR with the fungal universal primers ITS1 and ITS2 and sequenced. As negative results were obtained for 93% of the tissue specimens infected by\n                    <jats:italic>M. circinelloides<\/jats:italic>\n                    , the data for this species were excluded from the analysis. Positive PCR results were obtained for 93% (52\/56), 89% (50\/56), and 27% (15\/56) of the samples with 30, 10, and 1 slide cuts, respectively. There were minor differences, depending on the organ tissue, fungal species, and laboratory. Correct species identification was possible for 100% (30 cuts), 98% (10 cuts), and 93% (1 cut) of the cases. With the protocol used in the present study, the interlaboratory reproducibility of ITS sequencing for the identification of major\n                    <jats:italic>Zygomycetes<\/jats:italic>\n                    species from formalin-fixed paraffin-embedded tissues can reach 100%, when enough material is available.\n                  <\/jats:p>","DOI":"10.1128\/jcm.02319-09","type":"journal-article","created":{"date-parts":[[2010,4,7]],"date-time":"2010-04-07T22:20:41Z","timestamp":1270678841000},"page":"2043-2046","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":83,"title":["Molecular Detection and Identification of\n                    <i>Zygomycetes<\/i>\n                    Species from Paraffin-Embedded Tissues in a Murine Model of Disseminated Zygomycosis: a Collaborative European Society of Clinical Microbiology and Infectious Diseases (ESCMID) Fungal Infection Study Group (EFISG) Evaluation"],"prefix":"10.1128","volume":"48","author":[{"given":"Eric","family":"Dannaoui","sequence":"first","affiliation":[{"name":"Institut Pasteur, Unite\u0301 de Mycologie Mole\u0301culaire, Centre National de Re\u0301fe\u0301rence Mycologie et Antifongiques, CNRS URA3012, Paris, France"},{"name":"Universite\u0301 Paris Descartes, Faculte\u0301 de Me\u0301decine, AP-HP, Ho\u0302pital Europe\u0301en Georges Pompidou, Unite\u0301 de Parasitologie-Mycologie, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick","family":"Schwarz","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unite\u0301 de Mycologie Mole\u0301culaire, Centre National de Re\u0301fe\u0301rence Mycologie et Antifongiques, CNRS URA3012, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michal","family":"Slany","sequence":"additional","affiliation":[{"name":"Centre for Cardiovascular Surgery and Transplantation, Molecular Genetics 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Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Raghdaa","family":"Shrief","sequence":"additional","affiliation":[{"name":"National Aspergillosis Centre, Manchester Academic Health Science Centre, University of Manchester, Wythenshawe Hospital, Manchester, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michel","family":"Huerre","sequence":"additional","affiliation":[{"name":"Institut Pasteur, Unite\u0301 d'Histotechnologie et Pathologie, Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomas","family":"Freiberger","sequence":"additional","affiliation":[{"name":"Centre for Cardiovascular Surgery and Transplantation, Molecular Genetics Laboratory, Brno, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peter","family":"Gaustad","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Rikshospitalet University Hospital, Oslo, 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