{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T07:33:10Z","timestamp":1790839990266,"version":"4.1.0"},"reference-count":14,"publisher":"American Society for Microbiology","issue":"3","license":[{"start":{"date-parts":[[2017,3,1]],"date-time":"2017-03-01T00:00:00Z","timestamp":1488326400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            The performance of the Bactec FX blood culture system for detecting\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            bacteremia within the routine 1-week incubation period was assessed in a prospective study conducted in an area in southern Israel in which\n            <jats:named-content content-type=\"genus-species\">Brucella melitensis<\/jats:named-content>\n            is endemic. Aerobic vials (BD Bactec Plus Aerobic\/F medium) inoculated with blood specimens obtained from adult patients with positive Rose-Bengal screening test results were monitored for 4 consecutive weeks, and blind subcultures of negative vials were performed on solid media on days 7 and 28. During a 16-month period, a total of 31 (35.2%) of 88 cultures, obtained from 19 (38.0%) of 50 patients, were positive for\n            <jats:named-content content-type=\"genus-species\">Brucella melitensis<\/jats:named-content>\n            . The blood culture instrument identified 30 (96.8%) of 31 positive vials within 7 days of incubation; the single positive vial that was missed by the automated readings was detected only by the blind subculture performed on day 28. It is concluded that the Bactec FX system is able to detect the vast majority of episodes of\n            <jats:named-content content-type=\"genus-species\">Brucella<\/jats:named-content>\n            bacteremia within the 1-week incubation protocol instituted in most clinical microbiology laboratories and without the need to perform blind subcultures of negative vials, enabling early diagnosis and saving labor and incubation time and space.\n          <\/jats:p>","DOI":"10.1128\/jcm.02320-16","type":"journal-article","created":{"date-parts":[[2017,1,5]],"date-time":"2017-01-05T02:40:28Z","timestamp":1483584028000},"page":"942-946","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":29,"title":["The Bactec FX Blood Culture System Detects Brucella melitensis Bacteremia in Adult Patients within the Routine 1-Week Incubation Period"],"prefix":"10.1128","volume":"55","author":[{"given":"Moshe","family":"Sagi","sequence":"first","affiliation":[{"name":"Infectious Diseases Unit, Soroka University Medical Center, Ben-Gurion University of the Negev, Beer-Sheva, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1230-0428","authenticated-orcid":false,"given":"Lior","family":"Nesher","sequence":"additional","affiliation":[{"name":"Infectious Diseases Unit, Soroka University Medical Center, Ben-Gurion University of the Negev, Beer-Sheva, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pablo","family":"Yagupsky","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory, Soroka University Medical Center, Ben-Gurion University of the Negev, Beer-Sheva, Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra050570"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/21.2.283"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.2174\/1574891X11308010006"},{"key":"e_1_3_1_5_2","first-page":"1","article-title":"Laboratory techniques in brucellosis","volume":"55","author":"Alton GG","year":"1967","unstructured":"Alton GG Jones LM . 1967. Laboratory techniques in brucellosis. Monogr Ser World Health Organ55:1\u201392.","journal-title":"Monogr Ser World Health Organ"},{"key":"e_1_3_1_6_2","first-page":"382","volume-title":"Manual of clinical microbiology","author":"Hausker WJ","year":"1985","unstructured":"Hausker WJ Jr Moyer NP Holcomb LA . 1985. Brucella, p 382\u2013386. InLennetteEHBallowsAHauslerWJ JrShadomyEH (ed), Manual of clinical microbiology, 4th ed. 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Air Water Borne Dis2:112.","journal-title":"Air Water Borne Dis"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1089\/vbz.2011.0704"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00140-07"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-011-1325-8"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.28.5.1057-1059.1990"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02320-16","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02320-16","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T10:07:16Z","timestamp":1646474836000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02320-16"}},"subtitle":[],"editor":[{"given":"Karen C.","family":"Carroll","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"editor"}]}],"short-title":[],"issued":{"date-parts":[[2017,3]]},"references-count":14,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2017,3]]}},"alternative-id":["10.1128\/JCM.02320-16"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02320-16","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,3]]},"assertion":[{"value":"2016-11-21","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2016-12-28","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2017-02-22","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}