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Infect. Dis.<jats:bold>24:<\/jats:bold>388-398, 2005). The majority of them harbored the readily transmissible<jats:italic>mec<\/jats:italic>cassette type IV. Thus, theoretically, methicillin-susceptible<jats:italic>Staphylococcus aureus<\/jats:italic>(MSSA) might capture the<jats:italic>mecA<\/jats:italic>gene from circulating MRSA, or MRSA strains might catch mobile toxin genes from MSSA. Therefore, we characterized MSSA strains circulating at the University Hospital in Magdeburg. Among a total of 84 MSSA strains under study, about 40% possessed the<jats:italic>tst<\/jats:italic>(toxic shock syndrome toxin) gene and up to four additional enterotoxin genes.<jats:italic>tst<\/jats:italic>-positive MSSA strains belonged to all known<jats:italic>agr<\/jats:italic>groups (I to IV) and to 14 different<jats:italic>spa<\/jats:italic>types (t008, t012, t015, t019, t024, t056, t065, t127, t133, t162, t271, t287, t399, and t400), and they were classified by multilocus sequence typing (MLST) as ST1, ST8, ST30, ST39, ST45, ST101, ST121, ST395, and ST426. In contrast, simultaneously circulating MRSA strains (<jats:italic>n<\/jats:italic>= 24) harbored in general two or three genes of the enterotoxin gene cluster, and the<jats:italic>tst<\/jats:italic>-positive MRSA isolates belonged to the well-known epidemic types ST22, ST45, and ST228 and were classified as<jats:italic>spa<\/jats:italic>types t001, t028, and t032. From our results, one may conclude that the pool of circulating MSSA strains is an important parameter with regard to the epidemiology of hospital- and community-acquired MRSA clones and their potential virulence.<\/jats:p>","DOI":"10.1128\/jcm.02321-05","type":"journal-article","created":{"date-parts":[[2006,6,7]],"date-time":"2006-06-07T10:58:07Z","timestamp":1149677887000},"page":"2179-2185","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":44,"title":["Heterogeneity of Methicillin-Susceptible<i>Staphylococcus aureus<\/i>Strains at a German University Hospital Implicates the Circulating-Strain Pool as a Potential Source of Emerging Methicillin-Resistant<i>S. aureus<\/i>Clones"],"prefix":"10.1128","volume":"44","author":[{"given":"F.","family":"Layer","sequence":"first","affiliation":[{"name":"Institute of Medical Microbiology, Otto-von-Guericke University, Leipziger Str. 44, D-39120 Magdeburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Ghebremedhin","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Otto-von-Guericke University, Leipziger Str. 44, D-39120 Magdeburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Ko\u0308nig","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Otto-von-Guericke University, Leipziger Str. 44, D-39120 Magdeburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"B.","family":"Ko\u0308nig","sequence":"additional","affiliation":[{"name":"Institute of Medical Microbiology, Otto-von-Guericke University, Leipziger Str. 44, D-39120 Magdeburg, Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_1_2_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.10.5150-5157.2005"},{"key":"e_1_3_1_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/0966-842X(94)90608-4"},{"key":"e_1_3_1_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jb.165.2.373-378.1986"},{"key":"e_1_3_1_5_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.4.1434-1439.2003"},{"key":"e_1_3_1_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.9.2548-2553.1998"},{"key":"e_1_3_1_7_2","doi-asserted-by":"publisher","DOI":"10.3109\/10408419009105728"},{"key":"e_1_3_1_8_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.10.4735-4743.2004"},{"key":"e_1_3_1_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.42.5.2080-2084.2004"},{"key":"e_1_3_1_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0966-842X(99)01609-1"},{"key":"e_1_3_1_11_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.3.1008-1015.2000"},{"key":"e_1_3_1_12_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.161098098"},{"key":"e_1_3_1_13_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-005-1339-1"},{"key":"e_1_3_1_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0929-693X(01)80190-1"},{"key":"e_1_3_1_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.48.1.285-296.2004"},{"key":"e_1_3_1_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.12.5442-5448.2003"},{"key":"e_1_3_1_17_2","first-page":"221","year":"1999","unstructured":"Hiramatsu, K., T. 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