{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T12:47:28Z","timestamp":1762865248431},"reference-count":8,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2007,4,1]],"date-time":"2007-04-01T00:00:00Z","timestamp":1175385600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            We evaluated highly sensitive methods using boronic acid (BA) to detect extended-spectrum \u03b2-lactamase (ESBL) production. A total of 182 clinical isolates of\n            <jats:italic>Klebsiella<\/jats:italic>\n            spp. (\n            <jats:italic>n<\/jats:italic>\n            = 118) and\n            <jats:italic>Escherichia coli<\/jats:italic>\n            (\n            <jats:italic>n<\/jats:italic>\n            = 64) were analyzed: 62 harbored only ESBLs, 80 harbored both ESBLs and plasmid-mediated AmpC \u03b2-lactamases (pAmpCs), and 40 harbored only pAmpCs. The CLSI confirmatory test detected all isolates that produce only ESBLs but detected 85% of isolates that produce both enzymes. When a \u22655-mm increase in the zone diameter of either the cefotaxime (CTX) or the ceftazidime (CAZ) disk in the presence of both clavulanic acid (CA) and BA was considered to be a positive result, the test detected all isolates that harbor ESBLs (\u00b1 pAmpCs) but showed frequent false-positive results (50%) for isolates that produce only pAmpCs. Meanwhile, when a \u22653-mm increase in the zone diameter of either the CTX\/BA or the CAZ\/BA disk in the presence of CA was considered to be a positive result, the test also detected all isolates that harbor ESBLs (\u00b1 pAmpCs) and showed less frequent false-positive results (5%) in isolates that produce only pAmpCs. The latter new interpretive guideline has enhanced detection of ESBLs in clinical isolates of\n            <jats:italic>Klebsiella<\/jats:italic>\n            spp. and\n            <jats:italic>Escherichia coli<\/jats:italic>\n            and allowed detection of an ESBL even when potentially masked by a pAmpC.\n          <\/jats:p>","DOI":"10.1128\/jcm.02322-06","type":"journal-article","created":{"date-parts":[[2007,2,15]],"date-time":"2007-02-15T01:41:59Z","timestamp":1171503719000},"page":"1180-1184","update-policy":"http:\/\/dx.doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":56,"title":["Detection of Extended-Spectrum \u03b2-Lactamases by Using Boronic Acid as an AmpC \u03b2-Lactamase Inhibitor in Clinical Isolates of\n            <i>Klebsiella<\/i>\n            spp. and\n            <i>Escherichia coli<\/i>"],"prefix":"10.1128","volume":"45","author":[{"given":"Wonkeun","family":"Song","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine, Hallym University College of Medicine, Seoul, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Il Kwon","family":"Bae","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Research Institute for Antimicrobial Resistance, Kosin University College of Medicine, Busan, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"You-Nae","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Research Institute for Antimicrobial Resistance, Kosin University College of Medicine, Busan, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chae-Hoon","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine, Yeungnam University College of Medicine, Daegu, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang Hee","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences, School of Biotechnology and Environmental Engineering, Myongji University, Yongin, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seok Hoon","family":"Jeong","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine and Research Institute for Antimicrobial Resistance, Kosin University College of Medicine, Busan, Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2090229"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.4.933-951.2001"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki278"},{"key":"e_1_3_2_5_2","unstructured":"Clinical and Laboratory Standards Institute. 2006. Performance standards for antimicrobial disk susceptibility tests 9th ed. Approved Standard M2-A9. Clinical and Laboratory Standards Institute Wayne PA."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.8.4163-4167.2005"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2005.10.001"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dki324"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.diagmicrobio.2006.01.012"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02322-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02322-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T05:50:27Z","timestamp":1646459427000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02322-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,4]]},"references-count":8,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2007,4]]}},"alternative-id":["10.1128\/JCM.02322-06"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02322-06","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,4]]},"assertion":[{"value":"2006-11-15","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-02-05","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-04-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}