{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,12]],"date-time":"2026-02-12T07:44:11Z","timestamp":1770882251767,"version":"3.50.1"},"reference-count":63,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2007,5,1]],"date-time":"2007-05-01T00:00:00Z","timestamp":1177977600000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2007,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            There is an urgent need for improved diagnosis of leptospirosis, an emerging infectious disease which imparts a large disease burden in developing countries. We evaluated the use of\n            <jats:italic>Leptospira<\/jats:italic>\n            immunoglobulin (Ig)-like (Lig) proteins as a serodiagnostic marker for leptospirosis. Lig proteins have bacterial immunoglobulin-like (Big) tandem repeat domains, a moiety found in virulence factors in other pathogens. Sera from patients identified during urban outbreaks in Brazil reacted strongly with immunoblots of a recombinant fragment comprised of the second to sixth Big domains of LigB from\n            <jats:italic>L. interrogans<\/jats:italic>\n            serovar Copenhageni, the principal agent for transmission in this setting. Furthermore, the sera recognized an analogous LigB fragment derived from\n            <jats:italic>L. kirschneri<\/jats:italic>\n            serovar Grippotyphosa, a pathogenic serovar which is not endemic to the study area. The immunoblot assay detected anti-LigB IgM antibodies in sera from 92% (95% confidence interval, 85 to 96%) of patients during acute-phase leptospirosis. The assay had a sensitivity of 81% for sera from patients with less than 7 days of illness. Anti-LigB antibodies were found in sera from 57% of the patients who did not have detectable anti-whole-\n            <jats:italic>Leptospira<\/jats:italic>\n            responses as detected by IgM enzyme-linked immunosorbent assay and microagglutination test. The specificities of the assay were 93 to 100% and 90 to 97% among sera from healthy individuals and patients with diseases that have clinical presentations that overlap with those of leptospirosis, respectively. These findings indicate that the antibody response to this putative virulence determinant is a sensitive and specific marker for acute infection. The use of this marker may aid the prompt and timely diagnosis required to reduce the high mortality associated with severe forms of the disease.\n          <\/jats:p>","DOI":"10.1128\/jcm.02344-06","type":"journal-article","created":{"date-parts":[[2007,3,15]],"date-time":"2007-03-15T00:31:30Z","timestamp":1173918690000},"page":"1528-1534","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":80,"title":["<i>Leptospira<\/i>\n            Immunoglobulin-Like Proteins as a Serodiagnostic Marker for Acute Leptospirosis"],"prefix":"10.1128","volume":"45","author":[{"given":"Julio","family":"Croda","sequence":"first","affiliation":[{"name":"Gonc\u0327alo Moniz Research Center, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Salvador, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joa\u0303o G. R.","family":"Ramos","sequence":"additional","affiliation":[{"name":"Gonc\u0327alo Moniz Research Center, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Salvador, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James","family":"Matsunaga","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, California"},{"name":"Department of Medicine, UCLA School of Medicine, Los Angeles, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adriano","family":"Queiroz","sequence":"additional","affiliation":[{"name":"Gonc\u0327alo Moniz Research Center, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Salvador, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akira","family":"Homma","sequence":"additional","affiliation":[{"name":"Biomanguinhos, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Rio de Janeiro, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lee W.","family":"Riley","sequence":"additional","affiliation":[{"name":"School of Public Health, University of California, Berkeley, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A.","family":"Haake","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, Veterans Affairs Greater Los Angeles Healthcare System, Los Angeles, California"},{"name":"Department of Medicine, UCLA School of Medicine, Los Angeles, California"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitermayer G.","family":"Reis","sequence":"additional","affiliation":[{"name":"Gonc\u0327alo Moniz Research Center, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Salvador, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Albert I.","family":"Ko","sequence":"additional","affiliation":[{"name":"Gonc\u0327alo Moniz Research Center, Oswaldo Cruz Foundation, Brazilian Ministry of Health, Salvador, Brazil"},{"name":"Division of International Medicine and Infectious Disease, Weill Medical College of Cornell University, Ithaca, New York"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46652002000200005"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-11-4-387"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.2.803-809.2003"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(03)00830-2"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/CVI.00219-06"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2005.05.002"},{"key":"e_1_3_2_8_2","first-page":"133","volume":"23","year":"2005","unstructured":"Boonyod, D., Y. Poovorawan, P. Bhattarakosol, and C. Chirathaworn. 2005. LipL32, an outer membrane protein of Leptospira, as an antigen in a dipstick assay for diagnosis of leptospirosis. Asian Pac. J. Allergy Immunol.23:133-141.","journal-title":"Asian Pac. J. Allergy Immunol."},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.actatropica.2005.07.001"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0603979103"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1099\/00222615-25-4-269"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268800048779"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1999.61.731"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1015509105668"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3156.2006.01727.x"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.40.4.1464-1469.2002"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.33.2.419-427.1995"},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00155547"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(85)90246-9"},{"key":"e_1_3_2_20_2","unstructured":"Leptospira and leptospirosis 1999"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.9.3303-3310.2001"},{"key":"e_1_3_2_22_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2001.65.657"},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.69.8.4958-4968.2001"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.68.4.2276-2285.2000"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5438.291"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1006.030660"},{"key":"e_1_3_2_27_2","first-page":"249","volume":"51","year":"2003","unstructured":"Kaur, I. R., R. Sachdeva, V. Arora, and V. Talwar. 2003. Preliminary survey of leptospirosis amongst febrile patients from urban slums of East Delhi. J. Assoc. Physicians India51:249-251.","journal-title":"J. Assoc. Physicians India"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(99)80012-9"},{"key":"e_1_3_2_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2003.10.007"},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.3201\/eid1105.041212"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.14.2.296-326.2001"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2000.62.112"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.8.2.349-351.2001"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.1.11-14.1998"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1038\/35016618"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.29.4.805-809.1991"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.5.1735-1739.2000"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cimid.2006.06.007"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1086\/313501"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2958.2003.03619.x"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6215(00)00143-9"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.qco.0000178824.05715.2c"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/172.1.281"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1128\/JB.186.7.2164-2172.2004"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2005.07.015"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mcp.2004.10.002"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.45568-0"},{"key":"e_1_3_2_48_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.70.11.5924-5930.2002"},{"key":"e_1_3_2_49_2","doi-asserted-by":"publisher","DOI":"10.1089\/104454999314764"},{"key":"e_1_3_2_50_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.1.450-452.2000"},{"key":"e_1_3_2_51_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46652003000500002"},{"key":"e_1_3_2_52_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46651998000300009"},{"key":"e_1_3_2_53_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2002.66.605"},{"key":"e_1_3_2_54_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.3.1279-1282.2000"},{"key":"e_1_3_2_55_2","doi-asserted-by":"publisher","DOI":"10.1086\/427110"},{"key":"e_1_3_2_56_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268899002435"},{"key":"e_1_3_2_57_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.37.9.2904-2909.1999"},{"key":"e_1_3_2_58_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-3156.2001.00675.x"},{"key":"e_1_3_2_59_2","doi-asserted-by":"publisher","DOI":"10.1128\/CDLI.8.1.166-169.2001"},{"key":"e_1_3_2_60_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.38.3.1272-1275.2000"},{"key":"e_1_3_2_61_2","doi-asserted-by":"publisher","DOI":"10.1086\/314424"},{"key":"e_1_3_2_62_2","doi-asserted-by":"publisher","DOI":"10.1590\/S0036-46652002000600008"},{"key":"e_1_3_2_63_2","doi-asserted-by":"publisher","DOI":"10.1179\/000349804X3207"},{"key":"e_1_3_2_64_2","unstructured":"Human leptospirosis: guidance for diagnosis surveillance and control 2003"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02344-06","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02344-06","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T05:47:22Z","timestamp":1646459242000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02344-06"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,5]]},"references-count":63,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2007,5]]}},"alternative-id":["10.1128\/JCM.02344-06"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02344-06","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,5]]},"assertion":[{"value":"2006-11-19","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-03-06","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2007-05-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}