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A set of 287 strains representative of 133<jats:named-content content-type=\"genus-species\">Salmonella<\/jats:named-content>serovars was assembled to validate the array and to test the array probes for accuracy, specificity, and reproducibility. Initially, 76 known serovars were utilized to validate the specificity and repeatability of the array probes and their expected probe patterns. The SGSA generated the correct serovar designations for 100% of the known subspecies I serovars tested in the validation panel and an antigenic formula consistent with that of the White-Kauffmann-Le Minor scheme for 97% of all known serovars tested. Once validated, the SGSA was assessed against a blind panel of 100<jats:named-content content-type=\"genus-species\">Salmonella enterica<\/jats:named-content>subsp. I samples serotyped using traditional methods. In summary, the SGSA correctly identified all of the blind samples as representing<jats:named-content content-type=\"genus-species\">Salmonella<\/jats:named-content>and successfully identified 92% of the antigens found within the unknown samples. Antigen- and serovar-specific probes, in combination with a<jats:italic>pepT<\/jats:italic>PCR for confirmation of<jats:named-content content-type=\"genus-species\">S. enterica<\/jats:named-content>subsp. Enteritidis determinations, generated an antigenic formula and\/or a serovar designation consistent with the White-Kauffmann-Le Minor scheme for 87% of unknown samples tested with the SGSA. Future experiments are planned to test the specificity of the array probes with other<jats:named-content content-type=\"genus-species\">Salmonella<\/jats:named-content>serovars to demonstrate the versatility and utility of this array as a public health tool in the identification of<jats:named-content content-type=\"genus-species\">Salmonella<\/jats:named-content>.<\/jats:p>","DOI":"10.1128\/jcm.02347-10","type":"journal-article","created":{"date-parts":[[2011,6,23]],"date-time":"2011-06-23T00:16:27Z","timestamp":1308788187000},"page":"2954-2965","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":49,"title":["Rapid Genoserotyping Tool for Classification of Salmonella Serovars"],"prefix":"10.1128","volume":"49","author":[{"given":"Kristyn","family":"Franklin","sequence":"first","affiliation":[{"name":"Public Health Agency of Canada, Laboratory for Foodborne Zoonoses, 110 Stone Road West, Guelph, ON, Canada N1G 3W4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika J.","family":"Lingohr","sequence":"additional","affiliation":[{"name":"Public Health Agency of Canada, Laboratory for Foodborne Zoonoses, 110 Stone Road West, Guelph, ON, Canada N1G 3W4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Catherine","family":"Yoshida","sequence":"additional","affiliation":[{"name":"Public Health Agency of Canada, Laboratory for Foodborne Zoonoses, 110 Stone Road West, Guelph, ON, Canada N1G 3W4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muna","family":"Anjum","sequence":"additional","affiliation":[{"name":"Animal Health and Veterinary Laboratories Agency, New Haw, Addlestone, Surrey, United Kingdom"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Levente","family":"Bodrossy","sequence":"additional","affiliation":[{"name":"Department of Bioresources, Austrian Institute of Technology, A-2444 Seibersdorf, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clifford G.","family":"Clark","sequence":"additional","affiliation":[{"name":"Enteric Diseases Program, Bacteriology and Enteric Diseases Program, National Microbiology Laboratory, Public Health Agency of Canada, Winnipeg, MB, Canada R3E 3R2"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew M.","family":"Kropinski","sequence":"additional","affiliation":[{"name":"Public Health Agency of Canada, Laboratory for Foodborne Zoonoses, 110 Stone Road West, Guelph, ON, Canada N1G 3W4"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohamed A.","family":"Karmali","sequence":"additional","affiliation":[{"name":"Public Health Agency of Canada, Laboratory for Foodborne Zoonoses, 110 Stone Road West, Guelph, ON, Canada N1G 3W4"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_3_2_2","first-page":"WMC001299","article-title":"Incidences of endophytic human pathogens in fresh produce","volume":"1","author":"Ansingkar V.","year":"2010","unstructured":"AnsingkarV. 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