{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T02:10:58Z","timestamp":1790734258499,"version":"4.1.0"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2009,4,1]],"date-time":"2009-04-01T00:00:00Z","timestamp":1238544000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Methicillin-resistant\n            <jats:italic>Staphylococcus aureus<\/jats:italic>\n            (MRSA) has emerged as an important cause of skin and soft-tissue infections (SSTI). The understanding of the molecular epidemiology and virulence of MRSA continues to expand. From January 2005 to December 2005, we screened soldiers for MRSA nasal colonization, administered a demographic questionnaire, and monitored them prospectively for SSTI. All MRSA isolates underwent molecular analysis, which included pulsed-filed gel electrophoresis (PFGE) and PCR for Panton-Valentine leukocidin (PVL), the arginine catabolic mobile element (ACME), and the staphylococcal cassette chromosome\n            <jats:italic>mec<\/jats:italic>\n            (SCC\n            <jats:italic>mec<\/jats:italic>\n            ). Of the 3,447 soldiers screened, 134 (3.9%) had MRSA colonization. Of the 3,066 (89%) who completed the study, 39 developed culture-confirmed MRSA abscesses. Clone USA300 represented 53% of colonizing isolates but was responsible for 97% of the abscesses (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001). Unlike colonizing isolates, isolates positive for USA300, PVL, ACME, and type IV SCC\n            <jats:italic>mec<\/jats:italic>\n            were significantly associated with MRSA abscess isolates. As determined by multivariate analysis, risk factors for MRSA colonization were a history of SSTI and a history of hospitalization. Although various MRSA strains may colonize soldiers, USA300 is the most virulent when evaluated prospectively, and PVL, ACME, and type IV SCC\n            <jats:italic>mec<\/jats:italic>\n            are associated with these abscesses.\n          <\/jats:p>","DOI":"10.1128\/jcm.02352-08","type":"journal-article","created":{"date-parts":[[2009,2,12]],"date-time":"2009-02-12T03:04:59Z","timestamp":1234407899000},"page":"940-945","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":40,"title":["Presence and Molecular Epidemiology of Virulence Factors in Methicillin-Resistant\n            <i>Staphylococcus aureus<\/i>\n            Strains Colonizing and Infecting Soldiers"],"prefix":"10.1128","volume":"47","author":[{"given":"Michael W.","family":"Ellis","sequence":"first","affiliation":[{"name":"Department of Medicine (Infectious Diseases), Brooke Army Medical Center, Fort Sam Houston, San Antonio, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew E.","family":"Griffith","sequence":"additional","affiliation":[{"name":"Department of Medicine (Infectious Diseases), Brooke Army Medical Center, Fort Sam Houston, San Antonio, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"James H.","family":"Jorgensen","sequence":"additional","affiliation":[{"name":"Departments of Pathology"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duane R.","family":"Hospenthal","sequence":"additional","affiliation":[{"name":"Department of Medicine (Infectious Diseases), Brooke Army Medical Center, Fort Sam Houston, San Antonio, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katrin","family":"Mende","sequence":"additional","affiliation":[{"name":"Infectious Disease Clinical Research Program, Uniformed Services University of the Health Sciences, Bethesda, Maryland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan E.","family":"Patterson","sequence":"additional","affiliation":[{"name":"Departments of Pathology"},{"name":"Medicine (Infectious Diseases), University of Texas Health Science Center, San Antonio, Texas"},{"name":"South Texas Veterans Health Care System, San Antonio, Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3700501"},{"key":"e_1_3_2_3_2","unstructured":"Performance standards for antimicrobial disk susceptibility tests M2-A9 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