{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T12:12:57Z","timestamp":1777551177571,"version":"3.51.4"},"reference-count":31,"publisher":"American Society for Microbiology","issue":"10","license":[{"start":{"date-parts":[[2008,10,1]],"date-time":"2008-10-01T00:00:00Z","timestamp":1222819200000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2008,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>In a 2-month period in 2003, we encountered an outbreak of epidemic keratoconjunctivitis (EKC) in Japan. We detected 67 human adenoviruses (HAdVs) by PCR from eye swabs of patients with EKC at five eye clinics in different parts of Japan. Forty-one of the 67 HAdV DNAs from the swabs were identified as HAdV-37 by phylogenetic analysis using a partial hexon gene sequence. When the restriction patterns of these viral genomes were compared with that of the HAdV-37 prototype strain, one isolate showed a never-before-seen restriction pattern. Within 1 year, we encountered three more EKC cases caused by a genetically identical virus: two nosocomial infections at two different university hospitals and a sporadic infection at an eye clinic. We determined the nucleotide sequences of the full-length hexon and fiber genes of these isolates and compared them to those of the 51 prototype strains. Surprisingly, the sequence of the hexon (\u03b5 determinant) loop-1 and -2 regions showed the highest nucleotide identity with HAdV-22, a rare EKC isolate. However, the nucleotide sequence of the fiber gene was identical to that of the HAdV-8 prototype strain. 22 We propose that this virus is a new hexon-chimeric intermediate HAdV-22,37\/H8, and may be an etiological agent of EKC.<\/jats:p>","DOI":"10.1128\/jcm.02354-07","type":"journal-article","created":{"date-parts":[[2008,8,14]],"date-time":"2008-08-14T01:30:12Z","timestamp":1218677412000},"page":"3259-3269","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":45,"title":["Epidemic Keratoconjunctivitis Due to the Novel Hexon-Chimeric-Intermediate 22,37\/H8 Human Adenovirus"],"prefix":"10.1128","volume":"46","author":[{"given":"Koki","family":"Aoki","sequence":"first","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroaki","family":"Ishiko","sequence":"additional","affiliation":[{"name":"Host Defense Laboratory, Mitsubishi Chemical Medience Corporation, Tokyo 174-8535, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tsunetada","family":"Konno","sequence":"additional","affiliation":[{"name":"Host Defense Laboratory, Mitsubishi Chemical Medience Corporation, Tokyo 174-8535, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yasushi","family":"Shimada","sequence":"additional","affiliation":[{"name":"Host Defense Laboratory, Mitsubishi Chemical Medience Corporation, Tokyo 174-8535, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akio","family":"Hayashi","sequence":"additional","affiliation":[{"name":"Host Defense Laboratory, Mitsubishi Chemical Medience Corporation, Tokyo 174-8535, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisatoshi","family":"Kaneko","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Fukushima Medical University School of Medicine, Fukushima 960-1295, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Ohguchi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshitsugu","family":"Tagawa","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shigeaki","family":"Ohno","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology and Visual Sciences, Hokkaido University Graduate School of Medicine, Sapporo 060-8638, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shudo","family":"Yamazaki","sequence":"additional","affiliation":[{"name":"AIDS Vaccine Development Association, Tokyo 169-0075, Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF01314287"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1136\/bjo.66.12.776"},{"key":"e_1_3_2_4_2","first-page":"158","volume":"30","year":"1986","unstructured":"Aoki, K., R. 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