{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T13:21:53Z","timestamp":1790688113694,"version":"4.1.0"},"reference-count":35,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2009,5,1]],"date-time":"2009-05-01T00:00:00Z","timestamp":1241136000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2009,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:p>A novel method for the collection and transportation of dried-blood-plasma samples, SampleTanker (ST), was developed and compared to standard shipping protocols for frozen-plasma specimens containing human immunodeficiency virus type 1 (HIV-1) and\/or hepatitis C virus (HCV). Matched frozen and dried 1-ml EDTA-containing plasma samples were collected and analyzed by several molecular-based virologic assays. After addition of 1.175 ml of reconstitution buffer, 1.035 ml of dried plasma was recovered. Mean intra-assay variances were 0.05, 0.05, and 0.06 log<jats:sub>10<\/jats:sub>copies\/ml for the Versant, Amplicor, and NucliSens QT HIV-1 load assays, respectively (<jats:italic>P<\/jats:italic>, not significant). However, mean HIV-1 viral load was consistently reduced in dried samples by 0.32 to 0.51 log<jats:sub>10<\/jats:sub>copies\/ml, depending on assay type (<jats:italic>P<\/jats:italic>&lt; 0.05). Infectious HIV-1 was not recovered from dried ST plasma. There was no significant difference in HIV-1 viral load results obtained using ST after 8 weeks of storage at ambient temperature. Compared to frozen plasma, HIV-1 genotypic results were &gt;99% concordant at the nucleotide and amino acid levels, as well as for resistance-associated mutations. We further demonstrated successful detection of multiple analytes, including HIV-1 viral load, HIV-1 antiretroviral resistance genotype, and HCV genotype, from a single ST unit. Dried plasma collected with ST yielded comparable results to frozen samples for multiple-analyte clinical testing. As such, ST could be a useful alternative for virologic tests and clinical trials worldwide by significantly diminishing transportation cost and the sample volume restrictions associated with dried-blood-spot technology.<\/jats:p>","DOI":"10.1128\/jcm.02354-08","type":"journal-article","created":{"date-parts":[[2009,3,26]],"date-time":"2009-03-26T01:50:05Z","timestamp":1238032205000},"page":"1491-1496","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":24,"title":["Dried-Plasma Transport Using a Novel Matrix and Collection System for Human Immunodeficiency Virus and Hepatitis C Virus Virologic Testing"],"prefix":"10.1128","volume":"47","author":[{"suffix":"Jr.","given":"R. M.","family":"Lloyd","sequence":"first","affiliation":[{"name":"Research Think Tank, Inc., Buford, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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A.","family":"Winters","sequence":"additional","affiliation":[{"name":"VA Palo Alto Healthcare System, Palo Alto, California"},{"name":"Division of Infectious Diseases and Geographic Medicine, Stanford University, Palo Alto, California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Tanner","sequence":"additional","affiliation":[{"name":"Family HealthCare of Atlanta, Atlanta, Georgia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"De La Rosa","sequence":"additional","affiliation":[{"name":"Pharmasset, Inc., Princeton, New Jersey"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"B.","family":"Yen-Lieberman","sequence":"additional","affiliation":[{"name":"Cleveland Clinic Foundation, Cleveland, Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W.","family":"Armstrong","sequence":"additional","affiliation":[{"name":"Cleveland Clinic Foundation, Cleveland, Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"A.","family":"Taege","sequence":"additional","affiliation":[{"name":"Cleveland Clinic Foundation, Cleveland, Ohio"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. 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