{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,7]],"date-time":"2026-05-07T19:06:02Z","timestamp":1778180762014,"version":"3.51.4"},"reference-count":46,"publisher":"American Society for Microbiology","issue":"1","license":[{"start":{"date-parts":[[2013,1,1]],"date-time":"2013-01-01T00:00:00Z","timestamp":1356998400000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2013,1]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            In most areas where typhoid is endemic, laboratory diagnosis is not possible due to the lack of appropriate facilities. We investigated whether the combination of blood culture amplification of\n            <jats:named-content content-type=\"genus-species\">Salmonella enterica<\/jats:named-content>\n            serovar Typhi with an\n            <jats:named-content content-type=\"genus-species\">S. Typhi<\/jats:named-content>\n            antigen rapid diagnostic test (RDT) could be an accurate and inexpensive tool for the accelerated diagnosis of patients with acute typhoid in Laos. For a panel of 23 Gram-negative reference pathogens, the Standard Diagnostics (catalog no. 15FK20; Kyonggi-do, South Korea) RDT gave positive results for\n            <jats:named-content content-type=\"genus-species\">S. Typhi<\/jats:named-content>\n            NCTC 8385,\n            <jats:named-content content-type=\"genus-species\">S. Typhi<\/jats:named-content>\n            NCTC 786 (Vi negative),\n            <jats:named-content content-type=\"genus-species\">Salmonella enterica<\/jats:named-content>\n            serovar Enteritidis (ATCC 13076), and\n            <jats:named-content content-type=\"genus-species\">Salmonella enterica<\/jats:named-content>\n            serovar Ndolo NCTC 8700 (all group D). In a prospective study of 6,456 blood culture bottles from 3,028 patients over 15 months, 392 blood culture bottles (6.1%) from 221 (7.3%) patients had Gram-negative rods (GNRs) seen in the blood culture fluid. The sensitivity, negative predictive value, specificity, and positive predictive value were 96.7%, 99.5%, 97.9%, and 87.9%, respectively, for patients with proven\n            <jats:named-content content-type=\"genus-species\">S. Typhi<\/jats:named-content>\n            bacteremia and 91.2%, 98.4%, 98.9%, and 93.9% for patients with group D\n            <jats:named-content content-type=\"genus-species\">Salmonella<\/jats:named-content>\n            . The median (range) number of days between diagnosis by RDT and reference assays was 1 (\u22121 to +2) day for those with confirmed\n            <jats:named-content content-type=\"genus-species\">S. Typhi<\/jats:named-content>\n            . The use of antigen-based pathogen detection in blood culture fluid may be a useful, relatively rapid, inexpensive, and accurate technique for the identification of important causes of bacteremia in the tropics.\n          <\/jats:p>","DOI":"10.1128\/jcm.02360-12","type":"journal-article","created":{"date-parts":[[2012,10,25]],"date-time":"2012-10-25T04:29:53Z","timestamp":1351139393000},"page":"142-148","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":19,"title":["Evaluation of a Simple Blood Culture Amplification and Antigen Detection Method for Diagnosis of Salmonella enterica Serovar Typhi Bacteremia"],"prefix":"10.1128","volume":"51","author":[{"given":"Jos\u00e9e","family":"Castonguay-Vanier","sequence":"first","affiliation":[{"name":"Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic"},{"name":"Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Medicine, Churchill Hospital, University of Oxford, Oxford, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Viengmon","family":"Davong","sequence":"additional","affiliation":[{"name":"Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Latsanyphone","family":"Bouthasavong","sequence":"additional","affiliation":[{"name":"Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Davanh","family":"Sengdetka","sequence":"additional","affiliation":[{"name":"Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manivone","family":"Simmalavong","sequence":"additional","affiliation":[{"name":"Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amphayvanh","family":"Seupsavith","sequence":"additional","affiliation":[{"name":"Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David A. B.","family":"Dance","sequence":"additional","affiliation":[{"name":"Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic"},{"name":"Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Medicine, Churchill Hospital, University of Oxford, Oxford, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen","family":"Baker","sequence":"additional","affiliation":[{"name":"Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Medicine, Churchill Hospital, University of Oxford, Oxford, United Kingdom"},{"name":"Oxford University Clinical Research Unit, Hospital for Tropical Diseases, Ben Ham Tu, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tu","family":"Le Thi Phuong","sequence":"additional","affiliation":[{"name":"Oxford University Clinical Research Unit, Hospital for Tropical Diseases, Ben Ham Tu, Ho Chi Minh City, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Manivanh","family":"Vongsouvath","sequence":"additional","affiliation":[{"name":"Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul N.","family":"Newton","sequence":"additional","affiliation":[{"name":"Lao-Oxford-Mahosot Hospital-Wellcome Trust Research Unit, Microbiology Laboratory, Mahosot Hospital, Vientiane, Lao People's Democratic Republic"},{"name":"Centre for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Medicine, Churchill Hospital, University of Oxford, Oxford, United Kingdom"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"346","article-title":"The global burden of typhoid fever","volume":"82","author":"Crump JA","year":"2004","unstructured":"CrumpJA LubySP MintzED . 2004. The global burden of typhoid fever. Bull. World Health Organ. 82: 346\u2013 353.","journal-title":"Bull. World Health Organ."},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1586\/eri.11.47"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.333.7558.78"},{"key":"e_1_3_2_5_2","first-page":"78","article-title":"Latex agglutination test for rapid diagnosis of typhoid fever","volume":"8","author":"Kaur I","year":"1990","unstructured":"KaurI ThalwarV GuptaHC . 1990. Latex agglutination test for rapid diagnosis of typhoid fever. Indian J. Med. Microbiol. 8: 78\u2013 83.","journal-title":"Indian J. Med. Microbiol."},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.39.3.1002-1007.2001"},{"key":"e_1_3_2_7_2","first-page":"507","article-title":"Diagnostic value of dot-enzyme-immunoassay test to detect outer membrane protein antigen in sera of patients with typhoid fever","volume":"32","author":"Purwaningsih S","year":"2001","unstructured":"PurwaningsihS HandojoI PrihatiniS ProbohoesodoY . 2001. Diagnostic value of dot-enzyme-immunoassay test to detect outer membrane protein antigen in sera of patients with typhoid fever. Southeast Asian J. Trop. Med. Public Health 32: 507\u2013 512.","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.2471\/BLT.11.087627"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.36.6.1683-1687.1998"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-10-45"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1186\/1476-0711-9-14"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.31.6.1439-1443.1993"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2007.76.139"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0950268808000654"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00327-09"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2334-10-125"},{"key":"e_1_3_2_17_2","unstructured":"World Health Organization.\n2010.\nMalaria rapid diagnostic test performance: results of WHO product testing of malaria RDTs: round 2 (2009).\nWorld Health Organization Geneva Switzerland."},{"key":"e_1_3_2_18_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.65.2.452-456.1997"},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/0035-9203(80)90133-9"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.17.3.564-565.1983"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0732-8893(94)90068-X"},{"key":"e_1_3_2_22_2","first-page":"65","article-title":"Latex agglutination test (LAT) for the diagnosis of typhoid fever","volume":"36","author":"Kalhan R","year":"1999","unstructured":"KalhanR KaurI SinghRP GuptaHC . 1999. Latex agglutination test (LAT) for the diagnosis of typhoid fever. Indian Pediatr. 36: 65\u2013 68.","journal-title":"Indian Pediatr."},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1099\/jmm.0.47365-0"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2006.75.978"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2004.08.007"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2002.66.759"},{"key":"e_1_3_2_27_2","first-page":"166","volume-title":"Antigenic formulae of the Salmonella serovars","author":"Grimont PAD","year":"2007","unstructured":"GrimontPAD WeillF-X . 2007. Antigenic formulae of the Salmonella serovars, 9th ed, p 166. WHO Collaborating Centre for Reference and Research on Salmonella, Institut Pasteur, Paris, France.","edition":"9"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1002776"},{"key":"e_1_3_2_29_2","unstructured":"Labcare Diagnostics.\nAccessed 8 January 2012.\nLabcare Accucare S.Typhi-S.Paratyphi direct antigen detection.\nhttp:\/\/www.labcarediagnostics.com\/pdf\/Rapid%20Tests\/S.Typhi.pdf."},{"key":"e_1_3_2_30_2","unstructured":"Science With A Mission (SMI).\nAccessed 8 January 2012.\nScience with a Mission Salmonella Typhi antigen strip.\nhttp:\/\/www.sciencewithamission.org\/pdf_instructions\/SMI_typhoid_instructions.pdf."},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.326.7379.41"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhin.2012.01.006"},{"key":"e_1_3_2_33_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-9-31"},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2006.74.941"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-10-67"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1348-0421.2006.tb03843.x"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.25.7.1165-1168.1987"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.3.1158-1165.2005"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.62.9.3984-3993.1994"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1708-8305.2006.00094.x"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1086\/499363"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.trstmh.2007.06.004"},{"key":"e_1_3_2_43_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.43.5.2510-2512.2005"},{"key":"e_1_3_2_44_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10096-011-1370-3"},{"key":"e_1_3_2_45_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.16.6.1048-1051.1982"},{"key":"e_1_3_2_46_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.1999.61.658"},{"key":"e_1_3_2_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0272-2712(18)30403-7"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02360-12","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02360-12","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T11:32:08Z","timestamp":1646479928000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02360-12"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,1]]},"references-count":46,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2013,1]]}},"alternative-id":["10.1128\/JCM.02360-12"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02360-12","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,1]]},"assertion":[{"value":"2012-09-06","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-10-16","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2013-12-26","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}