{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T23:18:48Z","timestamp":1781306328057,"version":"3.54.1"},"reference-count":17,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2010,5,1]],"date-time":"2010-05-01T00:00:00Z","timestamp":1272672000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2010,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Diagnosis of strongyloidiasis using stool examination remains unsatisfactory due to the lack of sensitivity and fastidious techniques. In this work, we investigated the value of an anti-\n            <jats:italic>Strongyloides<\/jats:italic>\n            IgG enzyme immunoassay (EIA), using a panel of 207 sera retrospectively collected from patients with definitive diagnoses of strongyloidiasis (\n            <jats:italic>n<\/jats:italic>\n            = 57), other helminthic infections (\n            <jats:italic>n<\/jats:italic>\n            = 46), eosinophilia without parasitic infection diagnosis (\n            <jats:italic>n<\/jats:italic>\n            = 54), and digestive disturbances following a tropical journey (\n            <jats:italic>n<\/jats:italic>\n            = 30) and from 20 negative controls. By following a receiver operating characteristic (ROC) curve analysis, it was possible to optimize the test to reach a sensitivity of 91.2% and a specificity of 93.3%, with 92.8% of patients correctly classified. Considering the incidence of strongyloidiasis diagnosed in our own laboratory, the negative predictive value was calculated at 99.9%. In conclusion, this test is very rapid and easy to perform and may be valuable for diagnosis of strongyloidiasis both in cases where the infection is unrevealed by a parasitological stool examination and in patients at risk for severe clinical forms, such as patients receiving immunosuppressive therapy.\n          <\/jats:p>","DOI":"10.1128\/jcm.02364-09","type":"journal-article","created":{"date-parts":[[2010,3,25]],"date-time":"2010-03-25T03:25:10Z","timestamp":1269487510000},"page":"1716-1719","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":37,"title":["Evaluation of a Rapid Enzyme-Linked Immunosorbent Assay for Diagnosis of Strongyloidiasis"],"prefix":"10.1128","volume":"48","author":[{"given":"B.","family":"Bon","sequence":"first","affiliation":[{"name":"INSERM U945, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"S.","family":"Houze","sequence":"additional","affiliation":[{"name":"Assistance Publique-Ho\u0302pitaux de Paris, Ho\u0302pital Bichat, Service Parasitologie-Mycologie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H.","family":"Talabani","sequence":"additional","affiliation":[{"name":"Assistance Publique-Ho\u0302pitaux de Paris, Ho\u0302pital Cochin, Service Parasitologie-Mycologie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D.","family":"Magne","sequence":"additional","affiliation":[{"name":"INSERM U945, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"G.","family":"Belkadi","sequence":"additional","affiliation":[{"name":"INSERM U945, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Develoux","sequence":"additional","affiliation":[{"name":"INSERM U945, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y.","family":"Senghor","sequence":"additional","affiliation":[{"name":"Assistance Publique-Ho\u0302pitaux de Paris, Ho\u0302pital St. Antoine, Service Parasitologie-Mycologie, Paris, France"},{"name":"Assistance Publique-Ho\u0302pitaux de Paris, Ho\u0302pital Bichat, Service Parasitologie-Mycologie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Chandenier","sequence":"additional","affiliation":[{"name":"Service Parasitologie-Mycologie-Me\u0301decine Tropicale, CHRU, Tours, France, and INSERM U618, Tours, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Ancelle","sequence":"additional","affiliation":[{"name":"Assistance Publique-Ho\u0302pitaux de Paris, Ho\u0302pital Cochin, Service Parasitologie-Mycologie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Hennequin","sequence":"additional","affiliation":[{"name":"INSERM U945, Paris, France"},{"name":"Universite\u0301 Pierre et Marie Curie-Paris 6, UMR S945, Paris, France"},{"name":"Assistance Publique-Ho\u0302pitaux de Paris, Ho\u0302pital St. Antoine, Service Parasitologie-Mycologie, Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","first-page":"875","volume":"37","year":"2006","unstructured":"Asdamongkol, N., P. Pornsuriyasak, and S. Sungkanuparph. 2006. Risk factors for strongyloidiasis hyperinfection and clinical outcomes. Southeast Asian J. Trop. Med. Public Health37:875-884.","journal-title":"Southeast Asian J. Trop. Med. Public Health"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1469-0691.2009.02894.x"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1128\/jcm.34.10.2569-2571.1996"},{"key":"e_1_3_2_5_2","unstructured":"Diagnostic medical parasitology 2007"},{"key":"e_1_3_2_6_2","first-page":"87","volume":"15","year":"1991","unstructured":"Hennequin, C., G. Pialoux, C. Taillet-Bellemere, B. Caujolle, and J. P. Petite. 1991. Anguilluliasis hyperinfection in a patient receiving corticosteroids: hepatic and biliary involvement. Gastroenterol. Clin. Biol.15:87-88. (In French.)","journal-title":"Gastroenterol. Clin. Biol."},{"key":"e_1_3_2_7_2","first-page":"116","volume":"59","year":"1966","unstructured":"Junod, C. 1966. Description of a polyvalent method of parasitic enrichment of stools especially suitable for the diagnosis of tropical helminthiasis. Application to a series of people from the Antilles and of Laotians living in Paris. Bull. Soc. Pathol. Exot. Filiales59:116-123. (In French.)","journal-title":"Bull. Soc. Pathol. Exot. 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