{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,27]],"date-time":"2026-09-27T02:11:08Z","timestamp":1790475068299,"version":"4.1.0"},"reference-count":19,"publisher":"American Society for Microbiology","issue":"5","license":[{"start":{"date-parts":[[2017,5,1]],"date-time":"2017-05-01T00:00:00Z","timestamp":1493596800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Molecular diagnosis of congenital toxoplasmosis or disseminated toxoplasmosis is based mainly on PCR. The repeated DNA element\n            <jats:italic>rep529<\/jats:italic>\n            has become the main DNA target used in most PCR methods, whether laboratory developed or commercial. In this multicenter study, we evaluated the\n            <jats:named-content content-type=\"genus-species\">Toxoplasma<\/jats:named-content>\n            ELITe MGB (Elitech) commercial kit by comparison with three reference quantitative PCR assays (RAs) used routinely in three proficient laboratories of the French National Reference Center for Toxoplasmosis network, using\n            <jats:named-content content-type=\"genus-species\">Toxoplasma<\/jats:named-content>\n            calibrated suspensions diluted to obtain a range of concentrations from 0.1 to 10,000 parasites\/ml. These suspensions were extracted with either the DNA extraction kit (EXTRAblood; Elitech) recommended by the manufacturer or the QIAamp DNA minikit (Qiagen). The\n            <jats:named-content content-type=\"genus-species\">Toxoplasma<\/jats:named-content>\n            ELITe MGB assay was also evaluated on a panel of 128 clinical samples, including 56 amniotic fluid samples, 55 placenta samples, and various other samples, of which 95 originated from patients with proven toxoplasmosis. The ELITe MGB assay amplified low-concentration replicates (&lt;10 parasites\/ml) of calibrated suspensions less frequently than the RAs of 2\/3 laboratories. Additionally, the combination of EXTRAblood and\n            <jats:named-content content-type=\"genus-species\">Toxoplasma<\/jats:named-content>\n            ELITe MGB yielded poorer sensitivity than the combination of QIAamp DNA minikit and ELITe MGB for low parasite concentrations (\n            <jats:italic>P<\/jats:italic>\n            &lt; 0.001 for 1 parasite\/ml). On clinical samples, the sensitivity and the specificity of the commercial assay were 89% and 100%, respectively. The sensitivity ranged from 79% (placenta samples) to 100% (amniotic fluid samples). Overall, this study shows that the\n            <jats:named-content content-type=\"genus-species\">Toxoplasma<\/jats:named-content>\n            ELITe MGB assay is suitable for the diagnosis of toxoplasmosis from non-cell-rich or non-hemoglobin-rich samples and that the EXTRAblood kit is not optimal.\n          <\/jats:p>","DOI":"10.1128\/jcm.02379-16","type":"journal-article","created":{"date-parts":[[2017,2,16]],"date-time":"2017-02-16T01:50:18Z","timestamp":1487209818000},"page":"1369-1376","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":35,"title":["Evaluation of Toxoplasma ELITe MGB Real-Time PCR Assay for Diagnosis of Toxoplasmosis"],"prefix":"10.1128","volume":"55","author":[{"given":"Florence","family":"Robert-Gangneux","sequence":"first","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire de Rennes, Rennes, France"},{"name":"IRSET, INSERM U1085, Universit\u00e9 Rennes 1, Rennes, France"},{"name":"Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, P\u00f4le Biologie Mol\u00e9culaire, Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marie-Pierre","family":"Brenier-Pinchart","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Institut de Biologie et Pathologie, Centre Hospitalier Universitaire de Grenoble, Grenoble, France"},{"name":"Institute for Advanced Biosciences, INSERM U1209-CNRS UMR 5309, Universit\u00e9 Grenoble Alpes, Grenoble, France"},{"name":"Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, P\u00f4le Biologie Mol\u00e9culaire, Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"H\u00e9l\u00e8ne","family":"Yera","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, H\u00f4pital Cochin, H\u00f4pitaux Universitaires Paris Centre, AP-HP, Facult\u00e9 de M\u00e9decine, Universit\u00e9 Paris Descartes, Paris, France"},{"name":"Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, P\u00f4le Biologie Mol\u00e9culaire, Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sorya","family":"Belaz","sequence":"additional","affiliation":[{"name":"Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire de Rennes, Rennes, France"},{"name":"IRSET, INSERM U1085, Universit\u00e9 Rennes 1, Rennes, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emmanuelle","family":"Varlet-Marie","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Parasitologie-Mycologie, Centre Hospitalier Universitaire de Montpellier, and UMR CNRS 5290-IRD 224, Universit\u00e9 de Montpellier, Montpellier, France"},{"name":"Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, P\u00f4le Biologie Mol\u00e9culaire, Montpellier, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Bastien","sequence":"additional","affiliation":[{"name":"D\u00e9partement de Parasitologie-Mycologie, Centre Hospitalier Universitaire de Montpellier, and UMR CNRS 5290-IRD 224, Universit\u00e9 de Montpellier, Montpellier, France"},{"name":"Centre National de R\u00e9f\u00e9rence de la Toxoplasmose, P\u00f4le Biologie Mol\u00e9culaire, Montpellier, 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