{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T00:24:52Z","timestamp":1791246292820,"version":"4.1.0"},"reference-count":41,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2011,4,1]],"date-time":"2011-04-01T00:00:00Z","timestamp":1301616000000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"content-domain":{"domain":["journals.asm.org"],"crossmark-restriction":true},"short-container-title":["J Clin Microbiol"],"published-print":{"date-parts":[[2011,4]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n          <jats:p>\n            Alternative antischistosomal drugs are required since praziquantel is virtually the only drug available for treatment and morbidity control of schistosomiasis. Manual microscopic reading is the current \u201cgold standard\u201d to assess the\n            <jats:named-content content-type=\"genus-species\">in vitro<\/jats:named-content>\n            antischistosomal properties of test drugs; however, it is labor-intensive, subjective, and difficult to standardize. Hence, there is a need to develop novel tools for antischistosomal drug discovery. The\n            <jats:named-content content-type=\"genus-species\">in vitro<\/jats:named-content>\n            effects of praziquantel, oxamniquine, artesunate, and mefloquine on metabolic activity and parasite motility of\n            <jats:named-content content-type=\"genus-species\">Schistosoma mansoni<\/jats:named-content>\n            (newly transformed schistosomula [NTS] and 49-day-old adult worms) were studied using isothermal microcalorimetry (IMC). Results were compared to morphological readouts of viability. Results obtained for the four drugs tested with phenotypic evaluation by microscopy and IMC showed a good correlation, but IMC also identified drug effects that were not visible by microscopic evaluation, and IMC precisely determined the onset of action of the test drugs. Similar sensitivities on NTS and adult schistosomes were observed for praziquantel and mefloquine, while slight differences in the drug susceptibilities of the two developmental stages were noted with oxamniquine and artesunate. IMC is a useful tool for antischistosomal drug discovery that should be further validated. In addition, our data support the use of NTS in\n            <jats:named-content content-type=\"genus-species\">in vitro<\/jats:named-content>\n            antischistosomal drug assays.\n          <\/jats:p>","DOI":"10.1128\/jcm.02382-10","type":"journal-article","created":{"date-parts":[[2011,1,27]],"date-time":"2011-01-27T02:44:01Z","timestamp":1296096241000},"page":"1217-1225","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":61,"title":["Isothermal Microcalorimetry To Study Drugs against Schistosoma mansoni"],"prefix":"10.1128","volume":"49","author":[{"given":"Theresia","family":"Manneck","sequence":"first","affiliation":[{"name":"Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, P.O. Box, CH-4002 Basel, Switzerland"},{"name":"University of Basel, Petersplatz 1, CH-4003 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olivier","family":"Braissant","sequence":"additional","affiliation":[{"name":"Laboratory of Biomechanics and Biocalorimetry, c\/o Biozentrum\/Pharmazentrum, University of Basel, Klingelbergstrasse 50-70, 4056 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yolanda","family":"Haggenm\u00fcller","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, P.O. Box, CH-4002 Basel, Switzerland"},{"name":"University of Basel, Petersplatz 1, CH-4003 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Keiser","sequence":"additional","affiliation":[{"name":"Department of Medical Parasitology and Infection Biology, Swiss Tropical and Public Health Institute, P.O. Box, CH-4002 Basel, Switzerland"},{"name":"University of Basel, Petersplatz 1, CH-4003 Basel, Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000478"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/AAC.00640-09"},{"key":"e_1_3_2_4_2","doi-asserted-by":"publisher","DOI":"10.1517\/17460441.2.S1.S35"},{"key":"e_1_3_2_5_2","doi-asserted-by":"publisher","DOI":"10.3390\/s101009369"},{"key":"e_1_3_2_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.tube.2009.11.001"},{"key":"e_1_3_2_7_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.2009.01819.x"},{"key":"e_1_3_2_8_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182000047545"},{"key":"e_1_3_2_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2007.05.031"},{"key":"e_1_3_2_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-002-0751-z"},{"key":"e_1_3_2_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/0163-7258(95)00026-7"},{"key":"e_1_3_2_12_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182009000493"},{"key":"e_1_3_2_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(02)90405-0"},{"key":"e_1_3_2_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2010.08.008"},{"key":"e_1_3_2_15_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182010000405"},{"key":"e_1_3_2_16_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182009991739"},{"key":"e_1_3_2_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-308X(10)73008-6"},{"key":"e_1_3_2_18_2","unstructured":"Reference deleted."},{"key":"e_1_3_2_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkq233"},{"key":"e_1_3_2_20_2","doi-asserted-by":"publisher","DOI":"10.4269\/ajtmh.2010.10-0413"},{"key":"e_1_3_2_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0040-6031(88)87053-9"},{"key":"e_1_3_2_22_2","first-page":"405","volume-title":"Handbook of thermal analysis and calorimetry, vol. 4. From macromolecules to man","author":"Lamprecht I.","year":"1999","unstructured":"LamprechtI. SchmolzE.. 1999. Calorimetry of small animals, p. 405\u2013458. In KempR. B. (ed.), Handbook of thermal analysis and calorimetry, vol. 4. From macromolecules to man. Elsevier, Amsterdam, Netherlands."},{"key":"e_1_3_2_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.mimet.2008.04.004"},{"key":"e_1_3_2_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2010.08.011"},{"key":"e_1_3_2_25_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0031182009990965"},{"key":"e_1_3_2_26_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000795"},{"key":"e_1_3_2_27_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000504"},{"key":"e_1_3_2_28_2","doi-asserted-by":"publisher","DOI":"10.1017\/S0022149X08070235"},{"key":"e_1_3_2_29_2","first-page":"1132","article-title":"In vitro effects of hypothalamic-pituitary-adrenal axis (HPA) hormones on Schistosoma mansoni","volume":"87","author":"Morales-Montor J.","year":"2001","unstructured":"Morales-MontorJ.. 2001. In vitro effects of hypothalamic-pituitary-adrenal axis (HPA) hormones on Schistosoma mansoni. J. Parasitol. 87:1132\u20131139.","journal-title":"J. Parasitol."},{"key":"e_1_3_2_30_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0000759"},{"key":"e_1_3_2_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-4894(74)90076-9"},{"key":"e_1_3_2_32_2","doi-asserted-by":"publisher","DOI":"10.1517\/17460441.2.S1.S53"},{"key":"e_1_3_2_33_2","unstructured":"R Development Core Team. 2008. R: a language and environment for statistical computing. R Foundation for Statistical Computing Vienna Austria."},{"key":"e_1_3_2_34_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00436-006-0175-2"},{"key":"e_1_3_2_35_2","doi-asserted-by":"publisher","DOI":"10.1021\/jf981163y"},{"key":"e_1_3_2_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0020-7519(00)00031-X"},{"key":"e_1_3_2_37_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1473-3099(06)70521-7"},{"key":"e_1_3_2_38_2","doi-asserted-by":"publisher","DOI":"10.1517\/14656566.5.2.263"},{"key":"e_1_3_2_39_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2180-9-106"},{"key":"e_1_3_2_40_2","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00611-08"},{"key":"e_1_3_2_41_2","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/151.6.1130"},{"key":"e_1_3_2_42_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0035-9203(01)90336-0"}],"container-title":["Journal of Clinical Microbiology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02382-10","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/journals.asm.org\/doi\/pdf\/10.1128\/JCM.02382-10","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,3,5]],"date-time":"2022-03-05T12:59:00Z","timestamp":1646485140000},"score":1,"resource":{"primary":{"URL":"https:\/\/journals.asm.org\/doi\/10.1128\/JCM.02382-10"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,4]]},"references-count":41,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2011,4]]}},"alternative-id":["10.1128\/JCM.02382-10"],"URL":"https:\/\/doi.org\/10.1128\/jcm.02382-10","relation":{},"ISSN":["0095-1137","1098-660X"],"issn-type":[{"value":"0095-1137","type":"print"},{"value":"1098-660X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,4]]},"assertion":[{"value":"2010-11-24","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-01-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2011-04-04","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}