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Based on a review of the literature, we focused on three major inconsistencies between protocols: base medium, cell coculture, and temperature. Our data showed that\n            <jats:named-content content-type=\"genus-species\">Bartonella tamiae<\/jats:named-content>\n            demonstrated temperature-dependent growth limitations between common culturing conditions only 2\u00b0C apart. Additionally, growth of\n            <jats:named-content content-type=\"genus-species\">B. quintana<\/jats:named-content>\n            was significantly enhanced by the presence of mammalian cell coculture under mammalian cell culture conditions; however, when the medium was modified to incorporate insect cell culture-based medium, coculturing with mammalian cells was no longer needed. In this study, we were able to overcome these temperature- and cell-dependent limitations and accommodate all of the strains tested by combining mammalian cell culture-based medium with insect cell culture-based medium.\n          <\/jats:p>","DOI":"10.1128\/jcm.02403-10","type":"journal-article","created":{"date-parts":[[2011,2,3]],"date-time":"2011-02-03T02:02:34Z","timestamp":1296698554000},"page":"1363-1368","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":29,"title":["Combining Culture Techniques for Bartonella: the Best of Both Worlds"],"prefix":"10.1128","volume":"49","author":[{"given":"Tarah","family":"Lynch","sequence":"first","affiliation":[{"name":"Centers for Disease Control and Prevention, Division of Vector Borne Diseases, Fort Collins, Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Iverson","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Division of Vector Borne Diseases, Fort Collins, Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Kosoy","sequence":"additional","affiliation":[{"name":"Centers for Disease Control and Prevention, Division of Vector Borne Diseases, Fort Collins, Colorado"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetmic.2010.05.017"},{"key":"e_1_3_2_3_2","doi-asserted-by":"publisher","DOI":"10.1128\/iai.63.11.4553-4556.1995"},{"key":"e_1_3_2_4_2","first-page":"1-1","volume-title":"Current protocols in microbiology, unit 3C","author":"Battisti J. 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