{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T22:46:10Z","timestamp":1784241970575,"version":"3.55.0"},"reference-count":37,"publisher":"American Society for Microbiology","issue":"4","license":[{"start":{"date-parts":[[2017,4,1]],"date-time":"2017-04-01T00:00:00Z","timestamp":1491004800000},"content-version":"tdm","delay-in-days":0,"URL":"https:\/\/journals.asm.org\/non-commercial-tdm-license"}],"funder":[{"name":"CSIR, New Delhi","award":["F. No. 09\/174(0068)\/2014-EMR-I"],"award-info":[{"award-number":["F. No. 09\/174(0068)\/2014-EMR-I"]}]},{"name":"ICMR, New Delhi","award":["ICMR F. No. 80\/929\/2015-ECD-I"],"award-info":[{"award-number":["ICMR F. 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Of these, 72 (10%) isolates had clinical significance and were identified primarily by sequencing the internal transcribed spacer and large subunit (LSU) regions. MF represented 21 genera comprising 29 species, the majority of them belonging to the orders Pleosporales (50%) and Chaetothyriales (22%). Among the 29 fungal species identified in this study, only 6 (20%) species were identified by the MALDI-TOF MS due to the limited commercial database of Bruker Daltonics for MF. However, a 100% identification rate of 20 additional species identified in this study was obtained by constructing an in-house database using 24- to 96-h-old liquid cultures. Further, the CLSI broth microdilution method revealed low MICs for posaconazole (\u22641 \u03bcg\/ml) and voriconazole (\u22642 \u03bcg\/ml) in 96% and 95% of isolates, respectively. Skin\/subcutaneous and sino-nasal and pulmonary phaeohyphomycosis due to MF were diagnosed in 21.4% (\n            <jats:italic>n<\/jats:italic>\n            = 15) and 28.5% (\n            <jats:italic>n<\/jats:italic>\n            = 20) of cases. Also, 10% of patients had central nervous system involvement (\n            <jats:italic>n<\/jats:italic>\n            = 7), and 3 cases of fungal osteomyelitis due to\n            <jats:named-content content-type=\"genus-species\">Cladophialophora bantiana<\/jats:named-content>\n            and\n            <jats:named-content content-type=\"genus-species\">Corynespora<\/jats:named-content>\n            spp. were observed.\n          <\/jats:p>","DOI":"10.1128\/jcm.02413-16","type":"journal-article","created":{"date-parts":[[2017,1,19]],"date-time":"2017-01-19T02:20:35Z","timestamp":1484792435000},"page":"1090-1103","update-policy":"https:\/\/doi.org\/10.1128\/asmj-crossmark-policy-page","source":"Crossref","is-referenced-by-count":43,"title":["Molecular and Matrix-Assisted Laser Desorption Ionization\u2013Time of Flight Mass Spectrometry-Based Characterization of Clinically Significant Melanized Fungi in India"],"prefix":"10.1128","volume":"55","author":[{"given":"Ashutosh","family":"Singh","sequence":"first","affiliation":[{"name":"Department of Medical Mycology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pradeep Kumar","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Medical Mycology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anil","family":"Kumar","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Amrita Institute of Medical Sciences, Kochi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jagdish","family":"Chander","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Government Medical College Hospital, Chandigarh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Geetika","family":"Khanna","sequence":"additional","affiliation":[{"name":"Department of Pathology, Vardhman Mahavir Medical College and Safdarjung Hospital, Guru Gobind Singh Indraprastha University, New Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pradip","family":"Roy","sequence":"additional","affiliation":[{"name":"Department of Medical Mycology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3253-6080","authenticated-orcid":false,"given":"Jacques F.","family":"Meis","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology and Infectious Diseases, Canisius-Wilhelmina Hospital, Nijmegen, The Netherlands"},{"name":"Centre of Expertise in Mycology Radboudumc\/CWZ, Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2028-7462","authenticated-orcid":false,"given":"Anuradha","family":"Chowdhary","sequence":"additional","affiliation":[{"name":"Department of Medical Mycology, Vallabhbhai Patel Chest Institute, University of Delhi, Delhi, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"235","reference":[{"key":"e_1_3_2_2_2","doi-asserted-by":"crossref","first-page":"a019570","DOI":"10.1101\/cshperspect.a019570","article-title":"Black molds and melanized yeasts pathogenic to humans","volume":"5","author":"Chowdhary A","year":"2014","unstructured":"Chowdhary A Perfect J de Hoog GS . 2014. 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